PPP2R2C (NM_181876) Human Tagged ORF Clone

CAT#: RC214086

PPP2R2C (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_181876" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-PPP2R2C Antibody - N-terminal region
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms B55-GAMMA; B55gamma; IMYPNO; IMYPNO1; PR52; PR55G
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214086 representing NM_181876
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTGTCTTTTTTCTCCAAACACTTGCCCATCCAGGAAGGCCAGCCCTGGGCATTGAAGACTCCAG
CTGACATCATCTCTACCGTTGAGTTCAACCACACGGGAGAGCTGCTGGCCACAGGTGACAAGGGCGGCCG
GGTCGTCATCTTCCAGCGGGAACCAGAGAGTAAAAATGCGCCCCACAGCCAGGGCGAATACGACGTGTAC
AGCACTTTCCAGAGCCACGAGCCGGAGTTTGACTATCTCAAGAGCCTGGAGATAGAGGAGAAGATCAACA
AGATCAAGTGGCTCCCACAGCAGAACGCCGCCCACTCACTCCTGTCCACCAACGATAAAACTATCAAATT
ATGGAAGATTACCGAACGAGATAAAAGGCCCGAAGGATACAACCTGAAGGATGAAGAGGGGAAACTTAAG
GACCTGTCCACGGTGACGTCACTGCAGGTGCCAGTGCTGAAGCCCATGGATCTGATGGTGGAGGTGAGCC
CTCGGAGGATCTTTGCCAATGGCCACACCTACCACATCAACTCCATCTCCGTCAACAGTGACTGCGAGAC
CTACATGTCGGCGGATGACCTGCGCATCAACCTCTGGCACCTGGCCATCACCGACAGGAGCTTCAACATC
GTGGACATCAAGCCGGCCAACATGGAGGACCTTACGGAGGTGATCACAGCATCTGAGTTCCATCCGCACC
ACTGCAACCTCTTCGTCTACAGCAGCAGCAAGGGCTCCCTGCGGCTCTGCGACATGCGGGCAGCTGCCCT
GTGTGACAAGCATTCCAAGCTCTTTGAAGAGCCTGAGGACCCCAGTAACCGCTCATTCTTCTCGGAAATC
ATCTCCTCCGTGTCCGACGTGAAGTTCAGCCACAGCGGCCGCTACATGCTCACCCGGGACTACCTTACAG
TCAAGGTCTGGGACCTGAACATGGAGGCAAGACCCATAGAGACCTACCAGGTCCATGACTACCTTCGGAG
CAAGCTCTGTTCCCTGTACGAGAACGACTGCATTTTCGACAAGTTTGAATGTGCCTGGAACGGGAGCGAC
AGCGTCATCATGACCGGGGCCTACAACAACTTCTTCCGCATGTTCGATCGGAACACCAAGCGGGACGTGA
CCCTGGAGGCCTCGAGGGAAAGCAGCAAGCCCCGGGCTGTGCTCAAGCCACGGCGCGTGTGCGTGGGGGG
CAAGCGCCGGCGTGATGACATCAGTGTGGACAGCTTGGACTTCACCAAGAAGATCCTGCACACGGCCTGG
CACCCGGCTGAGAACATCATTGCCATCGCCGCCACCAACAACCTGTACATCTTCCAGGACAAGGTAAACT
CTGACATGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214086 representing NM_181876
Red=Cloning site Green=Tags(s)

MGLSFFSKHLPIQEGQPWALKTPADIISTVEFNHTGELLATGDKGGRVVIFQREPESKNAPHSQGEYDVY
STFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAHSLLSTNDKTIKLWKITERDKRPEGYNLKDEEGKLK
DLSTVTSLQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNI
VDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEI
ISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSD
SVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAW
HPAENIIAIAATNNLYIFQDKVNSDMH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_181876
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_181876.3
RefSeq Size 4087 bp
RefSeq ORF 1344 bp
Locus ID 5522
UniProt ID Q9Y2T4
Protein Families Druggable Genome, Phosphatase
Protein Pathways Tight junction
MW 51.2 kDa
Gene Summary The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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