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GIGYF1 (NM_022574) Human Tagged ORF Clone
CAT＃: RC213578
GIGYF1 (Myc-DDK-tagged)-Human GRB10 interacting GYF protein 1 (GIGYF1)
ORF Plasmid: tGFP
"NM_022574" in other vectors (5)
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CNY 7,208.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GYF1; PERQ1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213578 representing NM_022574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGAGACACTCAACTTTGGGCCTGAGTGGCTCAGGGCCCTGTCCGGGGGCGGCAGCGTGGCCT CCCCACCCCCGTCCCCTGCCATGCCCAAATACAAGCTGGCTGACTACCGTTATGGGCGAGAGGAAATGCT GGCTCTCTACGTCAAGGAGAACAAGGTCCCGGAAGAGCTGCAGGACAAGGAGTTCGCCGCGGTGCTGCAG GACGAGCCACTGCAGCCCCTGGCTCTGGAGCCGCTGACTGAGGAGGAACAGAGAAACTTCTCCCTGTCAG TGAACAGCGTGGCTGTGCTGAGGCTGATGGGGAAAGGGGCTGGCCCCCCCCTGGCTGGCACCTCCCGAGG CAGGGGCAGCACGCGGAGCCGAGGCCGCGGCCGTGGTGACAGCTGCTTTTACCAAAGAAGCATCGAAGAA GGCGATGGGGCCTTTGGACGAAGCCCCCGGGAAATCCAGCGCAGCCAGAGCTGGGATGACAGAGGCGAGA GGCGGTTTGAGAAGTCAGCAAGGCGGGATGGAGCACGATGTGGCTTTGAGGAGGGAGGGGCTGGCCCAAG GAAGGAGCACGCCCGCTCAGACAGCGAGAACTGGCGCTCCCTACGGGAGGAACAGGAGGAGGAGGAGGAG GGCAGCTGGAGGCTCGGAGCAGGGCCCCGGCGAGACGGCGACCGCTGGCGCTCCGCCAGCCCTGATGGTG GTCCCCGCTCTGCTGGCTGGCGGGAACATGGGGAACGGCGGCGCAAGTTTGAATTTGATTTGCGAGGGGA TCGAGGAGGGTGTGGTGAAGAGGAGGGGCGGGGAGGGGGAGGCAGCTCTCACCTGCGGCGGTGCCGAGCG CCTGAAGGCTTTGAGGAGGACAAGGATGGGCTCCCAGAGTGGTGCCTGGACGATGAGGATGAAGAAATGG GCACCTTTGATGCCTCTGGGGCCTTCTTGCCTCTCAAGAAGGGCCCCAAGGAGCCCATTCCTGAGGAGCA GGAGCTGGACTTCCAAGGGTTGGAGGAGGAGGAGGAACCTTCCGAAGGGCTAGAGGAGGAAGGGCCTGAG GCAGGTGGGAAAGAGCTGACCCCACTGCCTCCTCAGGAGGAGAAGTCCAGCTCCCCATCCCCACTGCCCA CCCTGGGCCCACTCTGGGGGACAAACGGGGATGGGGACGAAACTGCAGAGAAAGAGCCCCCAGCGGCCGA AGATGATATTCGGGGGATCCAGCTGAGTCCCGGGGTGGGCTCCTCTGCTGGCCCACCCGGAGATCTGGAG GATGATGAAGGCTTGAAGCACCTGCAGCAGGAGGCGGAGAAGCTGGTGGCCTCCCTGCAGGACAGCTCCT TGGAGGAGGAGCAGTTCACGGCTGCCATGCAGACCCAGGGCCTGCGCCACTCTGCAGCCGCCACTGCCCT CCCGCTCAGCCATGGGGCTGCCCGGAAGTGGTTCTACAAGGACCCACAGGGCGAGATCCAAGGCCCCTTC ACGACACAGGAGATGGCAGAGTGGTTCCAGGCCGGCTACTTTTCCATGTCACTGCTGGTGAAGCGGGGCT GCGATGAGGGCTTCCAGCCGCTGGGCGAGGTGATCAAGATGTGGGGCCGCGTGCCCTTTGCCCCAGGGCC CTCACCTCCCCCACTGCTGGGAAACATGGACCAGGAGCGGCTGAAGAAGCAACAGGAGCTGGCCGCGGCG GCCTTGTACCAGCAGCTGCAGCACCAGCAGTTTCTCCAGCTGGTCAGCAGCCGCCAGCTCCCGCAGTGCG CGCTCCGAGAAAAGGCAGCTCTGGGGGACCTGACACCGCCACCACCGCCGCCGCCACAGCAGCAGCAGCA GCAGCTCACGGCATTCCTGCAGCAGCTCCAGGCGCTCAAACCCCCCAGAGGCGGGGACCAGAACCTGCTC CCGACGATGAGCCGGTCCTTGTCGGTGCCAGATTCGGGCCGCCTCTGGGACGTACATACCTCAGCCTCAT CACAGTCAGGTGGTGAGGCCAGTCTTTGGGACATACCAATTAACTCTTCGACTCAGGGTCCAATTCTAGA ACAACTCCAGCTGCAACATAAATTCCAGGAGCGCAGAGAAGTGGAGCTCAGGGCGAAGCGGGAGGAAGAG GAACGCAAGCGTCGAGAGGAGAAGCGCCGCCAGCAGCAGCAGGAGGAGCAGAAGCGGCGGCAGGAGGAGG AAGAGCTGTTTCGGCGCAAGCACGTGCGGCAGCAGGAGCTATTGCTGAAGTTGCTACAGCAGCAGCAGGC GGTCCCTGTGCCCCCCGCACCCAGCTCCCCGCCCCCACTCTGGGCTGGCCTGGCCAAGCAGGGGCTGTCC ATGAAGACGCTCCTGGAGTTGCAGCTGGAGGGCGAGCGGCAGCTGCACAAACAGCCCCCACCTCGGGAGC CAGCTCGGGCCCAGGCCCCCAACCACCGAGTGCAGCTTGGGGGCCTGGGCACTGCCCCCCTGAACCAGTG GGTGTCTGAGGCTGGGCCACTGTGGGGCGGGCCAGACAAGAGTGGGGGCGGCAGCAGCGGCCTGGGGCTC TGGGAGGACACCCCCAAGAGCGGCGGGAGCCTGGTCCGTGGCCTCGGCCTGAAGAACAGCCGGAGCAGCC CATCTCTCAGTGACTCATACAGCCACCTATCGGGTCGGCCCATTCGCAAAAAGACGGAGGAAGAAGAGAA GCTGCTGAAGCTGCTGCAGGGCATTCCCAGGCCCCAGGACGGCTTCACCCAGTGGTGCGAGCAGATGCTG CACACGCTGAGCGCCACGGGCAGCCTGGACGTGCCCATGGCTGTAGCGATCCTCAAGGAGGTGGAATCCC CCTATGATGTCCACGATTATATCCGTTCCTGCCTGGGGGACACGCTGGAAGCCAAAGAATTTGCCAAACA ATTCCTGGAGCGGAGGGCCAAGCAGAAAGCCAGCCAGCAGCGGCAGCAGCAGCAGGAGGCATGGCTGAGC AGCGCCTCGCTGCAGACGGCCTTCCAGGCCAACCACAGCACCAAACTCGGCCCCGGGGAGGGCAGCAAGG CCAAGAGGCGGGCACTGATGCTGCACTCAGACCCCAGCATCCTGGGGTACTCCCTGCACGGATCTTCTGG TGAGATCGAGAGCGTGGATGACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213578 representing NM_022574
Red=Cloning site Green=Tags(s) MAAETLNFGPEWLRALSGGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKENKVPEELQDKEFAAVLQ DEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLAGTSRGRGSTRSRGRGRGDSCFYQRSIEE GDGAFGRSPREIQRSQSWDDRGERRFEKSARRDGARCGFEEGGAGPRKEHARSDSENWRSLREEQEEEEE GSWRLGAGPRRDGDRWRSASPDGGPRSAGWREHGERRRKFEFDLRGDRGGCGEEEGRGGGGSSHLRRCRA PEGFEEDKDGLPEWCLDDEDEEMGTFDASGAFLPLKKGPKEPIPEEQELDFQGLEEEEEPSEGLEEEGPE AGGKELTPLPPQEEKSSSPSPLPTLGPLWGTNGDGDETAEKEPPAAEDDIRGIQLSPGVGSSAGPPGDLE DDEGLKHLQQEAEKLVASLQDSSLEEEQFTAAMQTQGLRHSAAATALPLSHGAARKWFYKDPQGEIQGPF TTQEMAEWFQAGYFSMSLLVKRGCDEGFQPLGEVIKMWGRVPFAPGPSPPPLLGNMDQERLKKQQELAAA ALYQQLQHQQFLQLVSSRQLPQCALREKAALGDLTPPPPPPPQQQQQQLTAFLQQLQALKPPRGGDQNLL PTMSRSLSVPDSGRLWDVHTSASSQSGGEASLWDIPINSSTQGPILEQLQLQHKFQERREVELRAKREEE ERKRREEKRRQQQQEEQKRRQEEEELFRRKHVRQQELLLKLLQQQQAVPVPPAPSSPPPLWAGLAKQGLS MKTLLELQLEGERQLHKQPPPREPARAQAPNHRVQLGGLGTAPLNQWVSEAGPLWGGPDKSGGGSSGLGL WEDTPKSGGSLVRGLGLKNSRSSPSLSDSYSHLSGRPIRKKTEEEEKLLKLLQGIPRPQDGFTQWCEQML HTLSATGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFLERRAKQKASQQRQQQQEAWLS SASLQTAFQANHSTKLGPGEGSKAKRRALMLHSDPSILGYSLHGSSGEIESVDDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022574 |
| ORF Size | 3105 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022574.3, NP_072096.2 |
| RefSeq Size | 6329 bp |
| RefSeq ORF | 3108 bp |
| Locus ID | 64599 |
| UniProt ID | O75420 |
| MW | 114.4 kDa |
| Gene Summary | This gene encodes a member of the gyf family of adaptor proteins. The encoded protein contains a gyf protein interaction domain. It binds growth factor receptor bound 10, another adaptor protein that binds activated insulin-like growth factor 1 and insulin receptors and regulates receptor signaling. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213578L3 | Lenti ORF clone of Human GRB10 interacting GYF protein 1 (GIGYF1), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC213578L4 | Lenti ORF clone of Human GRB10 interacting GYF protein 1 (GIGYF1), mGFP tagged |
CNY 10,260.00 |
|
| RG213578 | GIGYF1 (tGFP-tagged) - Human GRB10 interacting GYF protein 1 (GIGYF1) |
CNY 8,808.00 |
|
| SC305038 | GIGYF1 (untagged)-Human GRB10 interacting GYF protein 1 (GIGYF1) |
CNY 19,950.00 |
|
| SC318456 | GIGYF1 (untagged)-Human GRB10 interacting GYF protein 1 (GIGYF1) |
CNY 7,216.00 |
