United States
Germany
Japan
United Kingdom
China
Myosin (MYL4) (NM_002476) Human Tagged ORF Clone
CAT＃: RC213170
MYL4 (Myc-DDK-tagged)-Human myosin, light chain 4, alkali, atrial, embryonic (MYL4), transcript variant 2
ORF Plasmid: tGFP
"NM_002476" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALC1; AMLC; GT1; PRO1957 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213170 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCAAGAAGCCTGAGCCTAAGAAGGAGGCAGCCAAGCCAGCTCCAGCTCCAGCTCCAGCCCCTG CACCAGCCCCTGCCCCAGCTCCTGAGGCTCCCAAGGAACCTGCCTTTGACCCCAAGAGTGTAAAGATAGA CTTCACTGCCGACCAGATTGAAGAGTTCAAAGAGGCCTTTTCATTGTTTGACCGGACCCCGACTGGAGAG ATGAAGATCACCTACGGCCAGTGCGGGGATGTACTGCGGGCCCTGGGCCAGAACCCTACCAATGCCGAGG TGCTGCGTGTGCTGGGCAAGCCCAAGCCTGAAGAGATGAATGTCAAGATGCTGGACTTTGAGACGTTCTT GCCCATCCTGCAGCACATTTCCCGCAACAAGGAGCAGGGCACCTATGAGGACTTCGTGGAGGGCCTGCGT GTCTTTGACAAGGAGAGCAATGGCACGGTCATGGGTGCTGAGCTTCGGCACGTCCTTGCCACCCTGGGAG AGAAGATGACTGAGGCTGAAGTGGAGCAGCTGTTAGCTGGGCAAGAGGATGCCAATGGCTGCATCAATTA TGAAGCCTTTGTCAAGCACATCATGTCAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213170 protein sequence
Red=Cloning site Green=Tags(s) MAPKKPEPKKEAAKPAPAPAPAPAPAPAPAPEAPKEPAFDPKSVKIDFTADQIEEFKEAFSLFDRTPTGE MKITYGQCGDVLRALGQNPTNAEVLRVLGKPKPEEMNVKMLDFETFLPILQHISRNKEQGTYEDFVEGLR VFDKESNGTVMGAELRHVLATLGEKMTEAEVEQLLAGQEDANGCINYEAFVKHIMSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002476 |
| ORF Size | 591 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002476.2, NP_002467.1 |
| RefSeq Size | 855 bp |
| RefSeq ORF | 594 bp |
| Locus ID | 4635 |
| UniProt ID | P12829 |
| MW | 21.6 kDa |
| Gene Summary | Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213170L1 | Lenti ORF clone of Human myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC213170L2 | Lenti ORF clone of Human myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC213170L3 | Lenti ORF clone of Human myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213170L4 | Lenti ORF clone of Human myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG213170 | MYL4 (tGFP-tagged) - Human myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant 2 |
CNY 4,000.00 |
|
| SC118608 | MYL4 (untagged)-Human myosin, light chain 4, alkali, atrial, embryonic (MYL4), transcript variant 2 |
CNY 2,400.00 |
