FGD3 (NM_001083536) Human Tagged ORF Clone

Name: FGD3 (NM_001083536) Human Tagged ORF Clone
SKU: RC212649
Price: 5312.00

CAT＃: RC212649

FGD3 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 3 (FGD3), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001083536" in other vectors (4)

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CNY 5,312.00

CNY 5,800.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA372565

FGD3 rabbit polyclonal antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZFYVE5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212649 representing NM_001083536 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGGCAGGGGGTCCTCAACCCCTCCAGGACCCATTGCTGCCCTAGGGATGCCAGACACTGGGC CTGGCAGTTCCTCCCTAGGGAAGCTTCAGGCGCTCCCTGTTGGGCCCAGAGCCCACTGTGGGGACCCTGT CAGCCTGGCTGCAGCAGGGGACGGCTCTCCAGACATAGGCCCCACGGGAGAGCTGAGTGGTAGCTTAAAG ATCCCCAACCGGGACAGCGGGATCGACAGTCCCTCCTCCAGTGTGGCTGGAGAGAACTTTCCCTGCGAGG AGGGCTTGGAGGCTGGCCCAAGCCCCACTGTACTGGGGGCGCACGCAGAGATGGCCCTGGACAGCCAGGT CCCGAAGGTCACCCCCCAGGAGGAGGCGGACAGCGACGTGGGTGAGGAACCTGACTCTGAGAACACCCCC CAGAAGGCTGACAAGGATGCCGGCCTGGCCCAGCACTCTGGCCCCCAGAAGCTTCTCCACATTGCCCAGG AGCTCCTGCACACCGAGGAGACCTATGTGAAGCGGCTGCACCTGCTGGACCAGGTTTTCTGCACCAGGCT GACGGATGCGGGGATCCCTCCAGAAGTCATCATGGGCATATTCTCTAACATCTCCTCCATCCACCGCTTC CACGGGCAGTTCCTGCTGCCGGAGCTGAAGACGCGGATCACGGAGGAGTGGGACACAAACCCACGGCTCG GGGACATCCTGCAGAAGCTGGCCCCATTCCTGAAGATGTACGGCGAGTATGTCAAGAACTTTGACCGAGC CGTAGGGCTGGTGAGCACGTGGACCCAGCGCTCCCCACTGTTTAAAGACGTCGTCCACAGCATCCAGAAG CAGGAGGTATGCGGGAACCTGACGCTGCAGCACCACATGCTGGAGCCCGTGCAGAGGGTCCCCCGGTACG AGCTGCTGCTCAAGGACTATCTGAAGAGGCTCCCGCAGGACGCCCCAGACCGGAAGGATGCGGAGAGGTC CTTGGAGCTCATCTCCACAGCCGCCAACCACTCCAATGCTGCCATTCGGAAAGTGGAGAAAATGCACAAG CTCTTGGAGGTGTACGAGCAGCTGGGTGGGGAAGAAGACATTGTCAACCCGGCCAATGAACTGATCAAGG AGGGCCAAATCCAGAAACTGTCAGCCAAGAACGGCACCCCCCAGGACCGCCACCTCTTCCTGTTCAACAG CATGATCCTTTACTGTGTGCCCAAGCTGCGGCTCATGGGCCAGAAGTTCAGCGTCCGGGAGAAGATGGAC ATCTCAGGCCTCCAGGTGCAGGATATCGTCAAGCCAAACACAGCACATACATTCATCATAACAGGAAGAA AAAGGTCCCTGGAGCTGCAGACGCGGACAGAGGAAGAGAAGAAAGAATGGATTCAGATCATCCAGGCCAC CATCGAGAAGCACAAACAGAACAGCGAAACCTTCAAGGCTTTTGGTGGCGCCTTCAGCCAGGATGAGGAC CCCAGCCTCTCTCCAGACATGCCTATCACGAGCACCAGCCCTGTGGAGCCTGTGGTGACCACCGAAGGCA GTTCGGGTGCAGCAGGGCTCGAGCCCAGAAAACTATCCTCTAAGACCAGACGTGACAAGGAGAAGCAGAG CTGTAAGAGCTGTGGTGAGACCTTCAACTCCATCACCAAGAGGAGGCATCACTGCAAGCTGTGTGGGGCG GTCATCTGTGGGAAGTGCTCCGAGTTCAAGGCCGAGAACAGCCGGCAGAGCCGTGTCTGCAGAGATTGTT TCCTGACACAGCCAGTGGCCCCTGAGAGCACAGAGAAGACACCCACTGCAGACCCCCAGCCCAGCCTGCT CTGCGGCCCCCTGCGGCTGTCAGAGAGCGGTGAGACCTGGAGCGAGGTGTGGGCCGCCATCCCCATGTCA GATCCCCAGGTGCTGCACCTGCAGGGAGGCAGCCAGGACGGCCGGCTGCCCCGCACCATCCCTCTCCCCA GCTGCAAACTGAGTGTGCCGGACCCTGAGGAGAGGCTGGACTCGGGGCATGTGTGGAAGCTGCAGTGGGC CAAGCAGTCCTGGTACCTGAGCGCCTCCTCCGCAGAGCTGCAGCAGCAGTGGCTGGAAACCCTAAGCACT GCTGCCCATGGGGACACGGCCCAGGACAGCCCGGGGGCCCTGCAGCTTCAGGTCCCTATGGGCGCAGCTG CTCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212649 representing NM_001083536 Red=Cloning site Green=Tags(s) MESGRGSSTPPGPIAALGMPDTGPGSSSLGKLQALPVGPRAHCGDPVSLAAAGDGSPDIGPTGELSGSLK IPNRDSGIDSPSSSVAGENFPCEEGLEAGPSPTVLGAHAEMALDSQVPKVTPQEEADSDVGEEPDSENTP QKADKDAGLAQHSGPQKLLHIAQELLHTEETYVKRLHLLDQVFCTRLTDAGIPPEVIMGIFSNISSIHRF HGQFLLPELKTRITEEWDTNPRLGDILQKLAPFLKMYGEYVKNFDRAVGLVSTWTQRSPLFKDVVHSIQK QEVCGNLTLQHHMLEPVQRVPRYELLLKDYLKRLPQDAPDRKDAERSLELISTAANHSNAAIRKVEKMHK LLEVYEQLGGEEDIVNPANELIKEGQIQKLSAKNGTPQDRHLFLFNSMILYCVPKLRLMGQKFSVREKMD ISGLQVQDIVKPNTAHTFIITGRKRSLELQTRTEEEKKEWIQIIQATIEKHKQNSETFKAFGGAFSQDED PSLSPDMPITSTSPVEPVVTTEGSSGAAGLEPRKLSSKTRRDKEKQSCKSCGETFNSITKRRHHCKLCGA VICGKCSEFKAENSRQSRVCRDCFLTQPVAPESTEKTPTADPQPSLLCGPLRLSESGETWSEVWAAIPMS DPQVLHLQGGSQDGRLPRTIPLPSCKLSVPDPEERLDSGHVWKLQWAKQSWYLSASSAELQQQWLETLST AAHGDTAQDSPGALQLQVPMGAAAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001083536
ORF Size	2175 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001083536.2
RefSeq Size	3470 bp
RefSeq ORF	2178 bp
Locus ID	89846
UniProt ID	Q5JSP0
Protein Pathways	Regulation of actin cytoskeleton
MW	79.4 kDa
Gene Summary	Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC212649L3	Lenti-ORF clone of FGD3 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 3 (FGD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RC212649L4	Lenti-ORF clone of FGD3 (mGFP-tagged)-Human FYVE, RhoGEF and PH domain containing 3 (FGD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
RG212649	FGD3 (tGFP-tagged) - Human FYVE, RhoGEF and PH domain containing 3 (FGD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00
SC316067	FGD3 (untagged)-Human FYVE, RhoGEF and PH domain containing 3 (FGD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,220.00

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