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eIF4GII (EIF4G3) (NM_003760) Human Tagged ORF Clone
CAT＃: RC212591
- TrueORF®
EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3
ORF Plasmid: tGFP
"NM_003760" in other vectors (4)
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CNY 10,848.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | eIF-4G 3; eIF4G 3; eIF4GII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212591 representing NM_003760
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCTAGAGCTACCA TCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCAGTGTACCAGGCTAATCAGCA CATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGGCCTCAGTACTGTATACCACAG TACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTC CTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCC GGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCAGCCAAGAGAGAGAAAAAA ACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCTGGAGGTGGCA GCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCCTCCTCAGCAGCTGCCCAGCCA GGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTC ACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAG TCCTTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGT ATCCATAGCAGAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTTGCTCGAAGTACAATTGCA GCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGATGTGAACTCT CATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACAGAAACATCAGACCCTTTACC AACAAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGTGTAGCACCTAATGATATTCCACTGGTT TCTAGTACTAACCTAATTAATGAAATAAATGGAGTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGG AAATAGTAAAACAGGAAGTATTGCCATTGACTCTTGAATTGGAGATTCTCGAAAATCCCCCAGAAGAAAT GAAACTGGAGTGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACT CCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTTAGTTCTCCGAGTG CTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTGCCTTAGTGAAGATGCAAA AGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACAGAAGAGATTTTGGATTCTCAAAACTTA AATTCAAGAAGGAGCCCTGTCCCAGCTCAAATAGCTATAACTGTACCAAAGACATGGAAGAAACCAAAAG ATCGGACCCGAACCACTGAAGAGATGTTAGAGGCAGAATTGGAGCTTAAAGCTGAAGAGGAGCTTTCCAT TGACAAAGTACTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAAAGAGACCCCTCTGAC CTAAAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGTGCTGAGAGTG TTTCTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCTGGTGATGGGGTTACATTTCC ATTTAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGTAAGAAGCAGTATGACAGGGAGTTTCTGCTG GACTTCCAGTTCATGCCTGCCTGTATACAAAAACCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTG ACAAGATCAACCAACCCAAATTGCCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCAGACTT TACACCAGCCTTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTTTGTTGAATGTTGGG TCACGAAGATCTCAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATCACAGTTTCTGTAAAAGAAGATG TACACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGACAGCCAAGCCGATGATCCCGA AAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGTATCTTAAATAAATTGACACCACAGATGTTC AATCAACTGATGAAGCAAGTGTCAGGACTTACTGTTGACACAGAGGAGCGGCTGAAAGGAGTTATTGACC TGGTCTTTGAGAAGGCTATTGATGAACCCAGTTTCTCTGTGGCTTACGCAAACATGTGTCGATGTCTAGT AACGCTGAAAGTACCCATGGCAGACAAGCCTGGTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGT TGCCAGAAGGAGTTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGAAGCAGAAAGAACTTGAGG CTGCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAAGCCAAGGACAAAGCCCGGCG GAGATCCATTGGCAACATCAAGTTTATTGGAGAACTCTTTAAACTCAAAATGCTGACTGAAGCCATCATG CATGACTGTGTGGTGAAGCTGCTAAAGAACCATGATGAAGAATCCCTGGAGTGCCTGTGTCGCCTGCTCA CCACCATTGGCAAAGACTTGGACTTTGAAAAAGCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGA GAAAATTGTGAAAGAAAGAAAAACCTCATCTAGGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGG CTGTGCAATTGGGTATCTCGAAGAGCAGATCAAGGGCCTAAAACTATCGAACAGATTCACAAAGAGGCTA AAATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAAGAGAAGAGAAGACCAGGTGT CCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGGGCCAAGAACAGTCGGGTACTGGACCCCTCA AAATTCCTAAAAATCACTAAGCCTACAATTGATGAAAAAATTCAGCTGGTACCTAAAGCACAGCTAGGCA GCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAGGCAAGTGAGACTGATGCCTTACGGTCAAGTGCTTCCAG TTTAAACAGATTCTCTGCCCTGCAACCTCCAGCACCCTCAGGGTCCACGCCATCCACGCCTGTAGAGTTT GATTCCCGAAGGACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATGACAAGCCCCTTCCATCTG CAACAGCTCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTGCTAGACAATCAGTCTCAAGA AGAGCAGCGGAGAGAGATGCTGGAGACCGTGAAGCAGCTCACAGGAGGTGTGGATGTGGAGAGGAACAGC ACTGAGGCTGAGCGAAATAAAACAAGGGAGTCAGCAAAACCAGAAATTTCAGCAATGTCAGCTCATGACA AGGCTGCATTATCAGAAGAGGAACTGGAGAGGAAGTCGAAATCTATCATTGATGAATTTCTACACATTAA TGATTTTAAGGAAGCCATGCAGTGTGTGGAAGAGCTGAATGCCCAGGGCCTACTACATGTTTTTGTGAGA GTGGGAGTGGAGTCCACCCTGGAAAGGAGCCAGATCACCAGGGATCACATGGGCCAACTACTCTATCAGC TGGTACAGTCAGAAAAACTCAGCAAACAGGACTTTTTCAAAGGTTTTTCAGAAACTTTGGAATTGGCAGA TGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTTGCTGAACTGGTGACCCCCATGTTAAAAGAA GGTGGAATCTCCATGAGAGAACTTACCATAGAATTTAGCAAACCTTTACTTCCTGTTGGAAGAGCTGGGG TCTTGCTATCTGAAATATTGCACCTACTATGCAAACAAATGAGCCATAAGAAAGTGGGAGCCTTATGGAG GGAGGCTGACCTCAGCTGGAAGGACTTTTTACCAGAAGGAGAAGATGTACATAATTTTCTTTTGGAGCAG AAGTTGGACTTCATAGAGTCTGACAGTCCCTGTTCCTCTGAAGCACTTTCAAAGAAAGAACTGTCTGCCG AAGAGCTGTATAAGCGACTCGAGAAACTCATTATTGAGGACAAAGCGAATGATGAACAGATCTTTGACTG GGTAGAGGCTAATCTAGACGAAATCCAGATGAGTTCACCTACATTCCTTAGAGCTTTAATGACTGCTGTT TGTAAAGCAGCTATTATAGCCGACTCTTCTACCTTCAGAGTGGACACTGCTGTTATCAAGCAGAGAGTGC CGATCTTACTCAAGTACCTAGACTCAGATACAGAGAAGGAACTGCAAGCACTTTATGCACTACAAGCATC GATAGTAAAACTTGATCAACCTGCCAATTTGCTGCGGATGTTTTTTGATTGTCTATATGACGAGGAGGTG ATCTCCGAGGATGCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCAGAGCAGAATGGGAAGGGCGTGG CTCTGAAATCTGTCACGGCATTCTTCACGTGGCTGCGGGAAGCAGAAGAGGAGTCTGAGGATAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212591 representing NM_003760
Red=Cloning site Green=Tags(s) MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQ YRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKK TIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPV TAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIA APTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLV SSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPT PPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNL NSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVLESEQDKMSQGFHPERDPSD LKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSGDGVTFPFKPESWKPTDTEGKKQYDREFLL DFQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVG SRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQADDPENIKTQELFRKVRSILNKLTPQMF NQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNR CQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIM HDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLR LCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPS KFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSTPSTPVEF DSRRTLTSRGSMGREKNDKPLPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNS TEAERNKTRESAKPEISAMSAHDKAALSEEELERKSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVR VGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKE GGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQ KLDFIESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAV CKAAIIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEV ISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003760 |
| ORF Size | 4755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003760.2, NP_003751.2 |
| RefSeq Size | 5880 bp |
| RefSeq ORF | 4758 bp |
| Locus ID | 8672 |
| UniProt ID | O43432 |
| Domains | eIF5C, MIF4G, MA3 |
| Protein Families | Transcription Factors |
| Protein Pathways | Viral myocarditis |
| MW | 176.5 kDa |
| Gene Summary | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212591L3 | Lenti-ORF clone of EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 17,670.00 |
|
| RC212591L4 | Lenti-ORF clone of EIF4G3 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 17,670.00 |
|
| RG212591 | EIF4G3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 12,448.00 |
|
| SC117780 | EIF4G3 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 3 |
CNY 10,856.00 |
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