ZNF33A (NM_006974) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KOX2; KOX5; KOX31; NF11A; ZNF11; ZNF11A; ZNF33; ZZAPK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC212054 representing NM_006974 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGGTAGAACAGAAGTCCCAGGAGTCAGTATCATTTAAAGATGTGACTGTGGGCTTCACCCAGG AGGAGTGGCAGCACCTGGACCCTAGTCAGAGGGCTCTGTATAGAGATGTGATGCTGGAGAACTACAGCAA CCTTGTCTCAGTGGGGTATTGTGTTCACAAACCAGAGGTGATCTTCAGGCTGCAACAAGGAGAAGAGCCA TGGAAACAGGAGGAAGAATTCCCAAGCCAAAGCTTTCCAGTCTGGACAGCTGATCACCTGAAAGAGAGGA GCCAAGAAAACCAATCTAAACATTTGTGGGAAGTTGTATTCATCAATAATGAAATGCTGACTAAGGAACA AGGTGATGTAATAGGAATACCATTTAATGTGGATGTAAGTTCTTTTCCTTCCAGAAAAATGTTCTGTCAG TGTGATTCATGTGGAATGAGTTTCAACACTGTTTCAGAATTGGTTATCAGTAAGATAAACTATTTAGGAA AAAAGTCTGATGAATTTAATGCCTGTGGGAAATTGTTACTCAATATTAAGCATGATGAAACTCATACTCA AGAGAAAAATGAAGTTTTGAAAAATAGGAACACACTGAGTCATCATGAGGAGACTTTGCAGCATGAGAAG ATTCAAACTTTAGAGCACAATTTTGAATACAGTATATGTCAGGAAACCCTCCTTGAAAAGGCAGTATTCA ATACACAGAAGAGAGAGAACGCAGAAGAGAATAACTGTGATTATAATGAATTTGGGAGAACTTTGTGTGA TAGTTCATCCCTCTTGTTCCATCAGATATCTCCGTCAAGGGACAATCACTATGAATTTAGTGATTGTGAG AAGTTCTTATGTGTGAAGTCCACCCTTTCTAAACCTCATGGGGTATCTATGAAACACTATGATTGTGGTG AAAGTGGGAATAATTTCAGGAGGAAATTGTGTCTGTCACACCTTCAGAAAGGTGATAAAGGAGAGAAACA CTTTGAATGTAATGAATGTGGGAAAGCTTTCTGGGAGAAGTCACATCTCACTCGACATCAGAGGGTGCAC ACAGGACAGAAACCCTTTCAATGTAATGAATGTGAAAAAGCTTTCTGGGATAAGTCAAACCTCACTAAAC ATCAAAGATCACACACAGGGGAGAAACCTTTTGAATGCAATGAATGTGGGAAAGCCTTTAGCCATAAGTC AGCCCTCACATTACACCAGAGAACACATACAGGGGAGAAACCCTATCAATGTAATGCGTGTGGGAAAACT TTTTGCCAGAAATCTGACCTCACTAAACATCAGAGAACACACACAGGGCTGAAACCCTATGAATGTTATG AATGTGGAAAATCCTTCCGTGTGACTTCGCACCTTAAAGTACACCAGAGAACTCACACAGGTGAGAAACC TTTTGAATGTCTTGAGTGTGGGAAATCCTTTAGTGAAAAGTCAAATCTTACACAGCATCAGAGAATTCAC ATAGGAGATAAATCTTATGAATGTAATGCATGTGGGAAAACTTTCTACCACAAGTCATTACTCACCAGGC ATCAGATAATTCATACAGGGTGGAAACCTTATGAATGTTATGAATGTGGGAAAACCTTCTGCTTGAAGTC AGACCTCACAGTACATCAGAGAACACACACAGGGCAGAAACCCTTTGCATGTCCCGAATGTGGGAAATTC TTTAGCCATAAGTCAACCCTCTCTCAACATTATAGAACACACACAGGGGAGAAACCCTACGAATGTCATG AATGTGGAAAAATCTTTTACAATAAATCATACCTAACTAAACATAATAGAACACATACAGGGGAGAAACC CTATGAATGTAATGAATGTGGAAAAGCCTTCTACCAGAAGTCACAACTCACTCAGCATCAGAGAATTCAC ATAGGGGAGAAACCCTATAAATGTAATGAGTGTGGAAAAGCTTTCTGCCATAAGTCAGCTCTAATTGTAC ATCAGAGAACCCATACACAAGAAAAGCCCTATAAATGTAATGAATGTGGAAAATCTTTCTGTGTAAAATC AGGACTTATTTTCCATGAGAGAAAGCACACGGGGGAGAAACCCTATGAATGCAATGAATGTGGGAAATTC TTCAGGCACAAATCATCACTCACAGTACATCACAGGGCTCACACAGGAGAGAAATCTTGTCAATGTAATG AATGTGGAAAAATCTTTTACCGTAAATCGGAACTTGCTCAACATCAGAGATCACATACAGGGGAAAAGCC CTATGAATGTAACACATGCAGGAAAACTTTCTCTCAAAAGTCAAATCTCATTGTACATCAGAGAAGACAT ATAGGAGAAAACCTTATGAATGAAATGGATATTAGAAATTTCCAGCCACAAGTCAGCCTCCATAATGCCT CAGAGTATTCACACTGTGGAGAAAGCCCTGATGACATCCTGAATGTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC212054 representing NM_006974 Red=Cloning site Green=Tags(s) MNKVEQKSQESVSFKDVTVGFTQEEWQHLDPSQRALYRDVMLENYSNLVSVGYCVHKPEVIFRLQQGEEP WKQEEEFPSQSFPVWTADHLKERSQENQSKHLWEVVFINNEMLTKEQGDVIGIPFNVDVSSFPSRKMFCQ CDSCGMSFNTVSELVISKINYLGKKSDEFNACGKLLLNIKHDETHTQEKNEVLKNRNTLSHHEETLQHEK IQTLEHNFEYSICQETLLEKAVFNTQKRENAEENNCDYNEFGRTLCDSSSLLFHQISPSRDNHYEFSDCE KFLCVKSTLSKPHGVSMKHYDCGESGNNFRRKLCLSHLQKGDKGEKHFECNECGKAFWEKSHLTRHQRVH TGQKPFQCNECEKAFWDKSNLTKHQRSHTGEKPFECNECGKAFSHKSALTLHQRTHTGEKPYQCNACGKT FCQKSDLTKHQRTHTGLKPYECYECGKSFRVTSHLKVHQRTHTGEKPFECLECGKSFSEKSNLTQHQRIH IGDKSYECNACGKTFYHKSLLTRHQIIHTGWKPYECYECGKTFCLKSDLTVHQRTHTGQKPFACPECGKF FSHKSTLSQHYRTHTGEKPYECHECGKIFYNKSYLTKHNRTHTGEKPYECNECGKAFYQKSQLTQHQRIH IGEKPYKCNECGKAFCHKSALIVHQRTHTQEKPYKCNECGKSFCVKSGLIFHERKHTGEKPYECNECGKF FRHKSSLTVHHRAHTGEKSCQCNECGKIFYRKSELAQHQRSHTGEKPYECNTCRKTFSQKSNLIVHQRRH IGENLMNEMDIRNFQPQVSLHNASEYSHCGESPDDILNVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_006974
ORF Size	2430 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006974.3
RefSeq Size	6127 bp
RefSeq ORF	2433 bp
Locus ID	7581
UniProt ID	Q06730
Protein Families	Transcription Factors, Transmembrane
MW	94.4 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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