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HYAL4 (NM_012269) Human Tagged ORF Clone
CAT＃: RC211151
HYAL4 (Myc-DDK-tagged)-Human hyaluronoglucosaminidase 4 (HYAL4)
ORF Plasmid: tGFP
"NM_012269" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CSHY; HYAL-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211151 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACTTCATGGCTCC TTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTTCCAATTTATCAAAGGAAACC TTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTGATAAAATATAATTTAAGACTAAATTTGAAA ATGTTTCCTGTGATTGGAAGCCCACTGGCCAAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACA GATTGGGATACTATCCGTGGTATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAG TTTACAAGTACATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAAGATGTTTACA GACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCTACCGATATTGAATATTTAGC CAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAGGAAACCATCAAATTGGGAATTAAGAGCCGA CCCAAAGGCCTTTGGGGTTATTATTTATATCCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACT CTGGGTCATGCCCAGAAGACGAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGC TTTATATCCTTCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTATTTGTCTACA CAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGATCTAGTCAGCACCATAGGAGA AAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGAGACATGAATTTAACTTCATCCAAGGCCAAC TGTACAAAGGTGAAGCAGTTTGTGAGTTCTGATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTG AGGTATGCAGCCTTCACCTCTGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTA CCTTCACTTGAACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGATATGAAGGAG CTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCTTCTCCTGGTTCACTAATGAC ACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211151 protein sequence
Red=Cloning site Green=Tags(s) MKVLSEGQLKLCVVQPVHLTSWLLIFFILKSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLK MFPVIGSPLAKARGQNVTIFYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSG LAVIDWEYWRPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSR PKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILRFSKF RVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAAGIVIWGDMNLTSSKAN CTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMWNAPSYLHLNPASYHIEASEDGEFTVKGK ASDTDLAVMADTFSCHCYQGYEGADCREIKTADGCSGVSPSPGSLMTLCLLLLASYRSIQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012269 |
| ORF Size | 1443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012269.3 |
| RefSeq Size | 2411 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 23553 |
| UniProt ID | Q2M3T9 |
| Domains | Glyco_hydro_56 |
| Protein Pathways | Glycosaminoglycan degradation, Metabolic pathways |
| MW | 54.3 kDa |
| Gene Summary | This gene encodes a protein which is similar in structure to hyaluronidases but lacks hyaluronidase activity. The encoded protein acts as a chondroitin-sulfate-specific endo-beta-N-acetylgalactosaminidase; that is, it exhibits hydrolytic activity toward chondroitin sulfate chains and degrades them into oligosaccharides. Proteoglycans are formed by the covalent linkage of chondroitin sulfate chains to protein. Proteoglycans are ubiquitous components of the extracellular matrix of connective tissues and are also found at the surface of many cell types where they participate in a variety of cellular processes such as cell proliferation, differentiation, migration, cell-cell recognition, extracellular matrix deposition, and tissue morphogenesis. The expression of this gene is highest in testes and placenta. [provided by RefSeq, Apr 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211151L1 | Lenti ORF clone of Human hyaluronoglucosaminidase 4 (HYAL4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211151L2 | Lenti ORF clone of Human hyaluronoglucosaminidase 4 (HYAL4), mGFP tagged |
CNY 5,890.00 |
|
| RC211151L3 | Lenti ORF clone of Human hyaluronoglucosaminidase 4 (HYAL4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC211151L4 | Lenti ORF clone of Human hyaluronoglucosaminidase 4 (HYAL4), mGFP tagged |
CNY 5,890.00 |
|
| RG211151 | HYAL4 (tGFP-tagged) - Human hyaluronoglucosaminidase 4 (HYAL4) |
CNY 4,370.00 |
|
| SC115433 | HYAL4 (untagged)-Human hyaluronoglucosaminidase 4 (HYAL4) |
CNY 3,656.00 |
