ARHGAP27 (NM_174919) Human Tagged ORF Clone
CAT#: RC209629
ARHGAP27 (Myc-DDK-tagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 3
ORF Plasmid: tGFP
"NM_174919" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CAMGAP1; PP905; SH3D20; SH3P20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209629 representing NM_174919
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGACGTGGTGGGGGACGTGTACGTGCTGGTGGAGCACCCCTTCGAGTACACCGGCAAGGACG GGCGCCGCGTGGCCATCCGGCCGAATGAGCGCTACCGGCTGCTGCGGCGCAGCACCGAGCACTGGTGGCA CGTGCGGCGTGAGCCCGGCGGCCGCCCCTTCTACCTGCCTGCGCAGTACGTGCGCGAGCTGCCCGCGCTG GGCAACCCTGCCGCCGCCGCGCCGCCAGGTCCCCACCCGAGCCCCGCGGCCCCTGAGCCGCTCGCCTACG ACTACCGGTTTGTGAGCGCGGCGGCGACCGCGGGCCCCGACGGCGCCCCCGAGGAGTCCGGAGGCCGAGC CAGCTCCCTGTGCGGCCCTGCGCAACGCGGCGCCGCGACCCAGCGCAGCAGCCTGGCGCCCGGCCTGCCA GCCTGCCTGTACCTGCGGCCCGCGGCGCCCGTGCGGCCCGCGCAGTCCCTGAACGACCTGGCCTGCGCCG CCGTCTCGCCTCCCGCCGGCCTCCTAGGAAGCAGCGGCAGCTTCAAGGCCTGCAGCGTGGCGGGCTCCTG GGTGTGCCCGCGGCCTCTGGCGCGCAGCGACTCAGAGAACGTCTACGAGGTCATCCAGGACTTGCACGTC CCGCCGCCGGAGGAGAGCGCAGAGCAGGTACCTCCCCGGGCGCTGGGGCGCGGAGGCGGGTGGCGCGCTA GGGACCGCGCCCGCACGGAGCCGGGGCGCAAGGAGACCCGCTCCGCTCAGCGTCGGGCACGACGCCCACC TCTGTCCGAAGACTTCGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209629 representing NM_174919
Red=Cloning site Green=Tags(s) MAADVVGDVYVLVEHPFEYTGKDGRRVAIRPNERYRLLRRSTEHWWHVRREPGGRPFYLPAQYVRELPAL GNPAAAAPPGPHPSPAAPEPLAYDYRFVSAAATAGPDGAPEESGGRASSLCGPAQRGAATQRSSLAPGLP ACLYLRPAAPVRPAQSLNDLACAAVSPPAGLLGSSGSFKACSVAGSWVCPRPLARSDSENVYEVIQDLHV PPPEESAEQVPPRALGRGGGWRARDRARTEPGRKETRSAQRRARRPPLSEDFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_174919 |
ORF Size | 789 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_174919.3, NP_777579.2 |
RefSeq Size | 1394 bp |
RefSeq ORF | 792 bp |
Locus ID | 201176 |
UniProt ID | Q6ZUM4 |
MW | 28.2 kDa |
Gene Summary | This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may pay a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209629L3 | Lenti-ORF clone of ARHGAP27 (Myc-DDK-tagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 3 |
CNY 5,890.00 |
|
RC209629L4 | Lenti-ORF clone of ARHGAP27 (mGFP-tagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 3 |
CNY 5,890.00 |
|
RG209629 | ARHGAP27 (tGFP-tagged) - Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 3 |
CNY 4,370.00 |
|
SC306956 | ARHGAP27 (untagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 3 |
CNY 3,990.00 |
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