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ASAP3 (NM_017707) Human Tagged ORF Clone
CAT＃: RC209195
ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1
ORF Plasmid: tGFP
"NM_017707" in other vectors (5)
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CNY 6,616.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACAP4; CENTB6; DDEFL1; UPLC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209195 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGCAGTTCAGCGTCGCCGAGTTCCTGGCCGTCACCGCGGAGGACCTCAGCTCCCCGGCTGGGG CCGCCGCCTTCGCCGCCAAGATGCCCCGGTACCGAGGGGCGGCGCTGGCGCGGGAGGAGATCTTGGAAGG AGACCAAGCCATCCTGCAGAGAATAAAGAAGGCTGTGCGGGCAATCCATAGCTCCGGCCTTGGCCATGTG GAGAATGAAGAGCAGTACCGAGAGGCCGTGGAATCCTTAGGCAACAGCCACCTGTCCCAGAACAGCCATG AGCTGTCCACAGGCTTCCTAAACTTGGCCGTGTTCACCCGCGAGGTTGCTGCGCTCTTCAAGAACCTGAT TCAGAACTTGAACAACATTGTCTCTTTCCCCCTGGACAGTCTGATGAAGGGGCAGCTGAGGGACGGTCGA CAGGATTCCAAAAAACAGCTGGAGAAGGCATGGAAGGACTATGAAGCCAAAATGGCCAAGCTGGAGAAGG AGCGCGATCGGGCCAGGGTGACAGGAGGGATCCCTGGGGAGGTGGCCCAGGACATGCAGAGAGAGCGGCG CATCTTCCAGCTGCACATGTGTGAGTATCTGCTCAAAGCCGGGGAGAGCCAGATGAAGCAAGGTCCTGAC TTCCTTCAGAGCCTCATCAAGTTCTTCCACGCCCAGCACAACTTTTTCCAAGATGGCTGGAAGGCTGCCC AGAGCCTGTTCCCCTTCATCGAGAAGCTGGCGGCCTCAGTACATGCACTCCATCAGGCCCAGGAGGACGA ACTACAGAAGCTGACCCAGCTCCGGGACTCCCTCCGAGGGACACTGCAGCTTGAGAGCAGAGAGGAACAC CTGAGCCGGAAGAACTCAGGATGTGGCTATAGCATCCACCAGCACCAAGGCAACAAGCAGTTTGGGACGG AGAAAGTGGGCTTTCTATACAAGAAAAGTGACGGAATTCGAAGAGCCTGGCAGAAAAGGAAGTGTGGAGT CAAGTATGGCTGCCTGACCATCTCACACAGCACGATAAACCGGCCCCCGGTGAAGCTGACCCTGCTGACG TGCCAAGTGAGGCCAAACCCTGAGGAGAAAAAGTGCTTCGACCTGGTGACCCACAACCGGACGTACCACT TTCAGGCAGAGGACGAGCACGAGTGTGAGGCGTGGGTGTCAGTGTTGCAGAACAGCAAGGACGAAGCCCT GAGCAGCGCCTTCCTCGGGGAGCCCAGCGCTGGCCCGGGGTCCTGGGGGTCCGCCGGCCATGATGGGGAG CCGCACGACCTCACAAAGCTGCTCATCGCGGAGGTGAAGAGCAGGCCTGGGAATAGCCAGTGCTGCGACT GCGGGGCTGCAGACCCCACGTGGCTCAGCACCAACCTGGGCGTGCTCACCTGCATCCAGTGCTCGGGCGT CCACCGCGAACTGGGCGTGCGCTTTTCGCGCATGCAGTCACTCACCTTGGACCTGCTGGGCCCCTCCGAG TTGTTGCTGGCCTTGAACATGGGAAACACGAGCTTCAATGAGGTCATGGAGGCCCAGCTACCCTCACACG GCGGCCCTAAACCCTCAGCTGAGAGTGACATGGGCACCCGCAGGGACTACATTATGGCCAAGTATGTGGA GCATAGGTTTGCACGCCGGTGCACACCTGAGCCTCAGCGACTCTGGACAGCCATTTGCAACAGGGACCTC CTGTCGGTACTGGAGGCCTTTGCCAATGGGCAGGACTTTGGACAGCCGCTGCCAGGGCCTGATGCACAGG CACCTGAAGAACTCGTCTTGCATTTGGCTGTCAAAGTCGCCAACCAGGCTTCCCTGCCTCTGGTGGATTT CATCATCCAGAACGGTGGTCACCTGGATGCCAAGGCTGCTGACGGGAACACGGCTCTGCACTACGCAGCA CTCTACAACCAGCCCGACTGCCTCAAGCTGCTGCTGAAGGGGAGAGCTTTGGTTGGCACAGTAAATGAAG CAGGCGAGACAGCTCTGGACATAGCCAGGAAGAAGCACCACAAGGAGTGTGAGGAGCTGCTGGAGCAGGC CCAGGCGGGGACCTTTGCCTTCCCTCTACATGTGGACTACTCCTGGGTAATTTCCACAGAGCCTGGCTCT GACAGTGAGGAGGATGAGGAAGAGAAGCGCTGCTTGCTGAAGCTCCCGGCCCAGGCTCACTGGGCCAGTG GGAGGCTGGACATCAGCAACAAGACCTATGAGACTGTCGCCAGCCTGGGAGCAGCCACCCCTCAGGGCGA GAGTGAGGACTGTCCCCCGCCCTTGCCAGTCAAAAACTCTTCTCGGACTTTGGTCCAAGGGTGTGCAAGA CATGCCAGTGGAGATCGTTCTGAAGTCTCCAGCCTGAGTTCAGAGGCCCCTGAGACCCCTGAGAGCCTGG GCAGTCCAGCCTCCTCCTCCAGTCTGATGAGCCCCTTGGAACCTGGGGATCCCAGCCAAGCCCCACCCAA CTCTGAAGAGGGCCTCCGAGAGCCCCCAGGCACCTCCAGACCCAGCCTGACATCCGGGACCACCCCTTCG GAGATGTACCTCCCCGTCAGATTCAGCTCCGAGAGCACTCGCTCCTATCGGCGGGGGGCGCGGAGCCCTG AAGATGGTCCCTCAGCCAGGCAGCCTCTGCCCAGAAGGAACGTGCCGGTTGGCATCACTGAAGGAGATGG CTCAAGGACTGGGAGTCTCCCAGCAAGTTCTGTGCAACTTTTGCAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209195 protein sequence
Red=Cloning site Green=Tags(s) MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVRAIHSSGLGHV ENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNLNNIVSFPLDSLMKGQLRDGR QDSKKQLEKAWKDYEAKMAKLEKERDRARVTGGIPGEVAQDMQRERRIFQLHMCEYLLKAGESQMKQGPD FLQSLIKFFHAQHNFFQDGWKAAQSLFPFIEKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEH LSRKNSGCGYSIHQHQGNKQFGTEKVGFLYKKSDGIRRAWQKRKCGVKYGCLTISHSTINRPPVKLTLLT CQVRPNPEEKKCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGE PHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSE LLLALNMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDL LSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAA LYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGS DSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCAR HASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPS EMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017707 |
| ORF Size | 2709 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017707.4 |
| RefSeq Size | 4199 bp |
| RefSeq ORF | 2712 bp |
| Locus ID | 55616 |
| UniProt ID | Q8TDY4 |
| Domains | ArfGap, PH, ANK |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
| MW | 99.1 kDa |
| Gene Summary | This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209195L3 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC209195L4 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RG209195 | ASAP3 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1 |
CNY 7,890.00 |
|
| SC113991 | ASAP3 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1 |
CNY 2,950.00 |
|
| SC318375 | ASAP3 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 1 |
CNY 7,220.00 |
