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SMARCAL1 (NM_014140) Human Tagged ORF Clone
CAT＃: RC208096
- TrueORF®
SMARCAL1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1
ORF Plasmid: tGFP
"NM_014140" in other vectors (7)
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CNY 10,480.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HARP; HHARP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208096 representing NM_014140
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTGCCTCTTACAGAGGAGCAGAGGAAAAAGATTGAAGAGAATCGACAAAAGGCTCTGGCCCGCA GAGCTGAGAAGTTATTGGCAGAACAGCATCAGAGGACTAGCTCGGGCACCTCCATTGCTGGCAACCCATT CCAGGCCAAGCAAGGCCCATCCCAAAATTTCCCAAGGGAGTCTTGTAAGCCAGTGAGCCATGGTGTCATT TTCAAGCAACAGAATCTCAGTAGCTCATCTAATGCTGACCAAAGACCTCATGATTCCCACAGTTTTCAGG CAAAGGGAATATGGAAAAAGCCAGAAGAAATGCCCACAGCCTGCCCAGGCCACAGTCCACGTAGTCAAAT GGCTCTCACTGGAATCTCTCCTCCCTTGGCACAAAGTCCTCCAGAGGTCCCTAAACAACAGCTCTTGAGT TATGAGTTAGGTCAAGGTCATGCTCAGGCTTCACCTGAGATCAGGTTCACACCCTTTGCTAACCCAACTC ATAAGCCTCTGGCCAAACCAAAGAGTTCCCAAGAGACACCAGCTCATTCCTCTGGACAGCCTCCCAGGGA TGCTAAGTTAGAGGCCAAGACAGCAAAAGCCTCCCCTTCGGGGCAGAACATTTCTTACATCCATTCTAGC TCAGAGAGTGTAACGCCCAGGACAGAAGGAAGACTCCAGCAGAAGTCAGGGTCCTCAGTCCAAAAAGGAG TGAACTCTCAGAAGGGAAAGTGCGTAAGGAACGGCGATCGTTTCCAGGTGTTGATTGGGTACAATGCGGA ACTCATTGCAGTGTTTAAGACCCTGCCCAGCAAGAATTATGATCCTGACACCAAGACGTGGAACTTCAGC ATGAATGACTATAGTGCCCTGATGAAAGCAGCCCAGAGCCTCCCCACGGTCAACCTGCAGCCTCTGGAAT GGGCCTATGGCAGCAGCGAGTCACCCTCCACCAGCAGTGAGGGACAGGCCGGCCTTCCATCAGCTCCATC CCTTTCATTTGTCAAAGGGCGATGCATGCTCATCTCCAGGGCCTACTTCGAGGCAGACATCAGTTATTCA CAGGACCTTATTGCGCTTTTTAAACAGATGGATTCCAGAAGATATGATGTCAAGACCAGGAAGTGGAGCT TTCTCTTGGAAGAGCACAGTAAACTAATTGCAAAGGTGCGCTGCCTCCCACAAGTTCAGCTGGACCCTCT GCCCACGACTCTCACCCTGGCGTTTGCTTCTCAGCTCAAGAAGACATCTCTCAGTCTCACGCCAGATGTC CCAGAGGCAGACCTTTCTGAAGTGGACCCCAAGCTCGTGTCTAATCTGATGCCCTTTCAGAGAGCTGGAG TCAATTTTGCCATAGCCAAAGGAGGCCGCCTGCTGCTCGCTGACGACATGGGCCTGGGGAAGACCATCCA AGCCATCTGCATCGCAGCCTTTTACCGGAAGGAGTGGCCGCTCCTGGTGGTGGTGCCATCCTCCGTGCGC TTCACCTGGGAGCAGGCCTTCCTTCGGTGGCTGCCATCTCTGAGCCCAGATTGCATCAACGTCGTGGTGA CTGGGAAGGACCGCCTGACAGCTGGCCTGATCAACATTGTCAGCTTTGACCTTCTTAGCAAGTTGGAAAA ACAGCTAAAAACCCCTTTTAAAGTTGTCATCATTGATGAATCTCACTTCCTCAAAAACAGTAGGACTGCC CGCTGTCGAGCAGCTATGCCGGTCCTAAAGGTTGCCAAGAGGGTGATCCTGTTGTCGGGCACACCAGCCA TGTCCCGGCCCGCAGAGCTCTACACGCAGATCATCGCAGTCAAGCCAACTTTCTTCCCCCAGTTTCATGC CTTTGGACTTCGCTACTGTGATGCCAAACGGATGCCTTGGGGGTGGGACTACTCAGGTTCCTCCAACCTG GGAGAGCTGAAGCTCCTGCTGGAGGAAGCAGTCATGCTGCGGCGCCTCAAGTCCGACGTCCTTTCCCAGC TGCCTGCCAAGCAGCGCAAGATAGTGGTGATTGCCCCAGGACGGATCAATGCCAGGACCAGAGCTGCCCT GGATGCTGCAGCCAAGGAAATGACCACCAAGGACAAAACTAAACAGCAGCAGAAAGATGCCCTCATTCTC TTCTTCAACAGAACAGCTGAAGCTAAAATCCCATCTGTCATTGAATATATCTTGGACCTACTGGAAAGTG GAAGAGAGAAGTTTTTAGTATTTGCACACCATAAGGTGGTCCTGGACGCAATTACGCAAGAGCTTGAGAG AAAGCACGTGCAGCACATCCGCATCGATGGCTCCACCTCATCAGCTGAGCGGGAGGACCTGTGCCAGCAG TTCCAACTGTCGGAGAGGCATGCTGTGGCCGTGCTGTCCATCACCGCTGCCAATATGGGCCTCACCTTCT CCTCGGCTGACCTGGTGGTGTTTGCTGAGCTGTTTTGGAACCCAGGGGTGCTGATCCAGGCTGAGGACCG CGTGCACCGCATTGGACAGACCAGCTCCGTGGGCATTCACTACCTCGTGGCAAAGGGCACAGCTGATGAC TACCTTTGGCCCCTGATTCAAGAGAAGATTAAAGTTCTGGCAGAAGCCGGGCTTTCTGAGACCAATTTTT CAGAAATGACAGAATCCACTGATTACCTCTACAAGGACCCAAAGCAGCAGAAGATCTACGACCTATTCCA GAAGTCCTTTGAGAAAGAAGGAAGTGATATGGAGCTCCTGGAAGCAGCAGAGTCCTTTGACCCAGGAAGT GCTTCAGGAACATCTGGAAGTAGTTCCCAGAACATGGGAGACACCCTGGATGAAAGCTCATTGACAGCCA GTCCACAGAAGAAAAGGAGATTTGAATTTTTTGATAACTGGGACAGCTTTACGTCTCCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208096 representing NM_014140
Red=Cloning site Green=Tags(s) MSLPLTEEQRKKIEENRQKALARRAEKLLAEQHQRTSSGTSIAGNPFQAKQGPSQNFPRESCKPVSHGVI FKQQNLSSSSNADQRPHDSHSFQAKGIWKKPEEMPTACPGHSPRSQMALTGISPPLAQSPPEVPKQQLLS YELGQGHAQASPEIRFTPFANPTHKPLAKPKSSQETPAHSSGQPPRDAKLEAKTAKASPSGQNISYIHSS SESVTPRTEGRLQQKSGSSVQKGVNSQKGKCVRNGDRFQVLIGYNAELIAVFKTLPSKNYDPDTKTWNFS MNDYSALMKAAQSLPTVNLQPLEWAYGSSESPSTSSEGQAGLPSAPSLSFVKGRCMLISRAYFEADISYS QDLIALFKQMDSRRYDVKTRKWSFLLEEHSKLIAKVRCLPQVQLDPLPTTLTLAFASQLKKTSLSLTPDV PEADLSEVDPKLVSNLMPFQRAGVNFAIAKGGRLLLADDMGLGKTIQAICIAAFYRKEWPLLVVVPSSVR FTWEQAFLRWLPSLSPDCINVVVTGKDRLTAGLINIVSFDLLSKLEKQLKTPFKVVIIDESHFLKNSRTA RCRAAMPVLKVAKRVILLSGTPAMSRPAELYTQIIAVKPTFFPQFHAFGLRYCDAKRMPWGWDYSGSSNL GELKLLLEEAVMLRRLKSDVLSQLPAKQRKIVVIAPGRINARTRAALDAAAKEMTTKDKTKQQQKDALIL FFNRTAEAKIPSVIEYILDLLESGREKFLVFAHHKVVLDAITQELERKHVQHIRIDGSTSSAEREDLCQQ FQLSERHAVAVLSITAANMGLTFSSADLVVFAELFWNPGVLIQAEDRVHRIGQTSSVGIHYLVAKGTADD YLWPLIQEKIKVLAEAGLSETNFSEMTESTDYLYKDPKQQKIYDLFQKSFEKEGSDMELLEAAESFDPGS ASGTSGSSSQNMGDTLDESSLTASPQKKRRFEFFDNWDSFTSPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014140 |
| ORF Size | 2862 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014140.4 |
| RefSeq Size | 3281 bp |
| RefSeq ORF | 2865 bp |
| Locus ID | 50485 |
| UniProt ID | Q9NZC9 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome |
| MW | 106.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208096L1 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1, Myc-DDK-tagged |
CNY 12,880.00 |
|
| RC208096L2 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208096L3 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208096L4 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1, mGFP tagged |
CNY 12,880.00 |
|
| RG208096 | SMARCAL1 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1 |
CNY 4,370.00 |
|
| SC115166 | SMARCAL1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1 |
CNY 2,950.00 |
|
| SC322645 | SMARCAL1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (SMARCAL1), transcript variant 1 |
CNY 2,950.00 |
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