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Rb2 p130 (RBL2) (NM_005611) Human Tagged ORF Clone
CAT＃: RC205380
RBL2 (Myc-DDK-tagged)-Human retinoblastoma-like 2 (p130) (RBL2)
ORF Plasmid: tGFP
"NM_005611" in other vectors (6)
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CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P130; Rb2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205380 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCGGGAGGTGACCAGTCGCCACCGCCCCCGCCTCCCCCTCCGGCGGCGGCAGCCTCGGATGAGG AGGAGGAGGACGACGGCGAGGCGGAAGACGCCGCGCCGCCTGCCGAGTCGCCCACCCCTCAGATCCAGCA GCGGTTCGACGAGCTGTGCAGCCGCCTCAACATGGACGAGGCGGCGCGGGCCGAGGCCTGGGACAGCTAC CGCAGCATGAGCGAAAGCTACACGCTGGAGGGAAATGATCTTCATTGGTTAGCATGTGCCTTATATGTGG CTTGCAGAAAATCTGTTCCAACTGTAAGCAAAGGGACAGTGGAAGGAAACTATGTATCTTTAACTAGAAT CCTGAAATGTTCAGAGCAGAGCTTAATCGAATTTTTTAATAAGATGAAGAAGTGGGAAGACATGGCAAAT CTACCCCCACATTTCAGAGAACGTACTGAGAGATTAGAAAGAAACTTCACTGTTTCTGCTGTAATTTTTA AGAAATATGAACCCATTTTTCAGGACATCTTTAAATACCCTCAAGAGGAGCAACCTCGTCAGCAGCGAGG AAGGAAACAGCGGCGACAGCCCTGTACTGTGTCTGAAATTTTCCATTTTTGTTGGGTGCTTTTTATATAT GCAAAAGGTAATTTCCCCATGATTAGTGATGATTTGGTCAATTCTTATCACCTGCTGCTGTGTGCTTTGG ACTTAGTTTATGGAAATGCACTTCAGTGTTCTAATCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCTT ATCTGAAGATTTTCATGCTAAAGATTCTAAACCTTCCTCTGACCCCCCTTGTATCATTGAGAAACTGTGT TCCTTACATGATGGCCTAGTTTTGGAAGCAAAGGGGATAAAGGAACATTTCTGGAAACCCTATATTAGGA AACTTTATGAAAAAAAGCTCCTTAAGGGAAAAGAAGAAAATCTCACTGGGTTTCTAGAACCTGGGAACTT TGGAGAGAGTTTTAAAGCCATCAATAAGGCCTATGAGGAGTATGTTTTATCTGTTGGGAATTTAGATGAG CGGATATTTCTTGGAGAGGATGCTGAGGAGGAAATTGGGACTCTCTCAAGGTGTCTGAACGCTGGTTCAG GAACAGAGACTGCTGAAAGGGTGCAGATGAAAAACATCTTACAGCAGCATTTTGACAAGTCCAAAGCACT TAGAATCTCCACACCACTAACTGGTGTTAGGTACATTAAGGAGAATAGCCCTTGTGTGACTCCAGTTTCT ACAGCTACGCATAGCTTGAGTCGTCTTCACACCATGCTGACAGGCCTCAGGAATGCACCAAGTGAGAAAC TGGAACAGATTCTCAGGACATGTTCCAGAGATCCAACCCAGGCTATTGCTAACAGACTGAAAGAAATGTT TGAAATATATTCTCAGCATTTCCAGCCAGACGAGGATTTCAGTAATTGTGCTAAAGAAATTGCCAGCAAA CATTTTCGTTTTGCGGAGATGCTTTACTATAAAGTATTAGAATCTGTTATTGAGCAGGAACAAAAAAGAC TAGGAGACATGGATTTATCTGGTATTCTGGAACAAGATGCGTTCCACAGATCTCTCTTGGCCTGCTGCCT TGAGGTCGTCACTTTTTCTTATAAGCCTCCTGGGAATTTTCCATTTATTACTGAAATATTTGATGTGCCT CTTTATCATTTTTATAAGGTGATAGAAGTATTCATTAGAGCAGAAGATGGCCTTTGTAGAGAGGTGGTAA AACACCTTAATCAGATTGAAGAACAGATCTTAGATCATTTGGCATGGAAACCAGAGTCTCCACTCTGGGA AAAAATTAGAGACAATGAAAACAGAGTTCCTACATGTGAAGAGGTCATGCCACCTCAGAACCTGGAAAGG GCAGATGAAATTTGCATTGCTGGCTCCCCTTTGACTCCCAGAAGGGTGACTGAAGTTCGTGCTGATACTG GAGGACTTGGAAGGAGCATAACATCTCCAACCACATTATACGATAGGTACAGCTCCCCACCAGCCAGCAC TACCAGAAGGCGGCTATTTGTTGAGAATGATAGCCCCTCTGATGGAGGGACGCCTGGGCGCATGCCCCCA CAGCCCCTAGTCAATGCTGTCCCTGTGCAGAATGTATCTGGGGAGACTGTTTCTGTCACACCAGTTCCTG GACAGACTTTGGTCACCATGGCAACCGCCACTGTCACAGCCAACAATGGGCAAACGGTAACCATTCCTGT GCAAGGTATTGCCAATGAAAATGGAGGGATAACATTCTTCCCTGTCCAAGTCAATGTTGGGGGGCAGGCA CAAGCTGTGACAGGCTCCATCCAGCCCCTCAGTGCTCAGGCCCTGGCTGGAAGTCTGAGCTCTCAACAGG TGACAGGAACAACTTTGCAAGTCCCTGGTCAAGTGGCCATTCAACAGATTTCCCCAGGTGGCCAACAGCA GAAGCAAGGCCAGTCTGTAACCAGCAGTAGTAATAGACCCAGGAAGACCAGCTCTTTATCGCTTTTCTTT AGAAAGGTATACCATTTAGCAGCTGTCCGCCTTCGGGATCTCTGTGCCAAACTAGATATTTCAGATGAAT TGAGGAAAAAAATCTGGACCTGCTTTGAATTCTCCATAATTCAGTGTCCTGAACTTATGATGGACAGACA TCTGGACCAGTTATTAATGTGTGCCATTTATGTGATGGCAAAGGTCACAAAAGAAGATAAGTCCTTCCAG AACATTATGCGTTGTTATAGGACTCAGCCGCAGGCCCGGAGCCAGGTGTATAGAAGTGTTTTGATAAAAG GAAAAAGAAAAAGAAGAAATTCTGGCAGCAGTGATAGCAGAAGCCATCAGAATTCTCCAACAGAACTAAA CAAAGATAGAACCAGTAGAGACTCCAGTCCAGTTATGAGGTCAAGCAGCACCTTGCCAGTTCCACAGCCC AGCAGTGCTCCTCCCACACCTACTCGCCTCACAGGTGCCAACAGTGACATGGAAGAAGAGGAGAGGGGAG ACCTCATTCAGTTCTACAACAACATCTACATCAAACAGATTAAGACATTTGCCATGAAGTACTCACAGGC AAATATGGATGCTCCTCCACTCTCTCCCTATCCATTTGTAAGAACAGGCTCCCCTCGCCGAATACAGTTG TCTCAAAATCATCCTGTCTACATTTCCCCACATAAAAATGAAACAATGCTTTCTCCTCGAGAAAAGATTT TCTATTACTTCAGCAACAGTCCTTCAAAGAGACTGAGAGAAATTAATAGTATGATACACACAGGAGAAAC TCCTACTAAAAAGAGAGGAATTCTTTTGGAAGATGGAAGTGAATCACCTGCAAAAAGAATTTGCCCAGAA AATCATTCTGCCTTATTACGCCGTCTCCAAGATGTAGCTAATGACCGTGGTTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205380 protein sequence
Red=Cloning site Green=Tags(s) MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSY RSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMAN LPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIY AKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLC SLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDE RIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVS TATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASK HFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVP LYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLER ADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPP QPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQA QAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFF RKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQ NIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQP SSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQL SQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIHTGETPTKKRGILLEDGSESPAKRICPE NHSALLRRLQDVANDRGSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005611 |
| ORF Size | 3417 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005611.4 |
| RefSeq Size | 4903 bp |
| RefSeq ORF | 3420 bp |
| Locus ID | 5934 |
| UniProt ID | Q08999 |
| Domains | RB_B, RB_A, CYCLIN |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cell cycle, TGF-beta signaling pathway |
| MW | 128.3 kDa |
| Gene Summary | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205380L1 | Lenti ORF clone of Human retinoblastoma-like 2 (p130) (RBL2), Myc-DDK-tagged |
CNY 10,328.00 |
|
| RC205380L2 | Lenti ORF clone of Human retinoblastoma-like 2 (p130) (RBL2), mGFP tagged |
CNY 11,690.00 |
|
| RC205380L3 | Lenti ORF clone of Human retinoblastoma-like 2 (p130) (RBL2), Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC205380L4 | Lenti ORF clone of Human retinoblastoma-like 2 (p130) (RBL2), mGFP tagged |
CNY 11,690.00 |
|
| RG205380 | RBL2 (tGFP-tagged) - Human retinoblastoma-like 2 (p130) (RBL2) |
CNY 9,528.00 |
|
| SC317004 | RBL2 (untagged)-Human retinoblastoma-like 2 (p130) (RBL2) |
CNY 7,936.00 |
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