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Dynein intermediate chain 1 (DNAI1) (NM_012144) Human Tagged Lenti ORF Clone
CAT＃: RC205242L4
- LentiORF®
Lenti ORF clone of Human dynein, axonemal, intermediate chain 1 (DNAI1), mGFP tagged
"NM_012144" in other vectors (6)
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CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | CILD1; DIC1; ICS1; PCD |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC205242).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012144 |
| ORF Size | 2097 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012144.2 |
| RefSeq Size | 2593 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 27019 |
| UniProt ID | Q9UI46 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| Protein Pathways | Huntington's disease |
| MW | 79.3 kDa |
| Gene Summary | This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205242 | DNAI1 (Myc-DDK-tagged)-Human dynein, axonemal, intermediate chain 1 (DNAI1) |
CNY 5,800.00 |
|
| RC205242L1 | Lenti ORF clone of Human dynein, axonemal, intermediate chain 1 (DNAI1), Myc-DDK-tagged |
CNY 7,520.00 |
|
| RC205242L2 | Lenti ORF clone of Human dynein, axonemal, intermediate chain 1 (DNAI1), mGFP tagged |
CNY 7,700.00 |
|
| RC205242L3 | Lenti ORF clone of Human dynein, axonemal, intermediate chain 1 (DNAI1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RG205242 | DNAI1 (tGFP-tagged) - Human dynein, axonemal, intermediate chain 1 (DNAI1) |
CNY 6,370.00 |
|
| SC107912 | DNAI1 (untagged)-Human dynein, axonemal, intermediate chain 1 (DNAI1) |
CNY 2,950.00 |
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