Dynein intermediate chain 1 (DNAI1) (NM_012144) Human Untagged Clone

CAT#: SC107912

DNAI1 (untagged)-Human dynein, axonemal, intermediate chain 1 (DNAI1)



  "NM_012144" in other vectors (6)

CNY 5,128.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
DNAI1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CILD1; DIC1; ICS1; PCD
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_012144, the custom clone sequence may differ by one or more nucleotides


ATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGAA
CCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAAGC
CACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATTTTG
ACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAGCCTA
TTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCAGATGA
AGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGGTTCTCAGGAGTCTGTCAAGGTGATTTCAGAA
ACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCTGGGAGTCAAACAGATGTGC
CTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCTAAGCAGCCCAAGGAGAGAAA
GCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTACAACAACCCTGTCCGGGATCGAGAA
TGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAATCAGTGGGAGATCTATGATGCCT
ATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAGGCAAAGACCCCAGTGGCTAAAAA
ATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAGACTGATGATCTCATCAAATTGTCC
CAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATATGATGACATTGCTCAAGATTTTAAGT
ACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACCCTGCTGCCGCTCTGGAAGTTCCAAAA
TGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAATCCAAAGTACAGGGATCTGTTTGCAGTG
GGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATGCTGCTGCTCTACAGCCTGAAGAACCCCA
GCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATGTGTCTCGACATCCACGTGGACCACCCCTA
CCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATTTACAACCTCAAGAAGCCCCACTCCCAGCCC
TCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGACCCTGTGTGGCAGGTCAAGTGGCAGAAGGATG
ACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCTGACGGCAGGATTGTGTCTTGGACTCTCGTGAA
GAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAGGTGGAAGGCAGCACCACGGAAGTTCCTGAGGGG
TTGCAGCTGCACCCAGTGGGTTGTGGCACTGCCTTTGACTTCCACAAAGAGATTGACTACATGTTCCTAG
TGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCTAAATCCTACTCCAGCCAATTCCTCGACACCTATGA
CGCCCACAACATGTCAGTGGACACTGTGTCCTGGAACCCATACCACACCAAGGTCTTCATGTCCTGCAGC
TCCGACTGGACAGTGAAGATCTGGGACCACACCATCAAGACCCCGATGTTCATCTATGACCTGAACTCAG
CCGTGGGTGATGTGGCCTGGGCGCCATACTCTTCTACTGTGTTCGCAGCAGTCACCACAGATGGGAAGGC
CCACATATTTGACTTAGCCATCAACAAGTATGAGGCCATCTGCAACCAGCCTGTGGCGGCCAAAAAGAAC
AGGCTCACCCACGTGCAGTTCAATCTCATCCACCCCATCATCATTGTGGGCGATGACCGTGGGCACATCA
TCAGCCTCAAGCTCTCACCCAATTTGCGCAAGATGCCAAAGGAAAAGAAGGGGCAGGAGGTGCAGAAGGG
TCCAGCTGTGGAGATTGCGAAACTGGACAAACTGCTGAACCTGGTGAGGGAAGTGAAAATCAAGACCTGA


>OriGene 5' read for NM_012144 unedited
TTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGACGGCTGTCCCTAAG
AACCGTTGCGACTGGTAACTGAAGTGGAAGAGAGTCCAGATTTCTTGTGTGTGGTCAAGG
AGACGGACAAACTTTTTGTCTTCAGACGAGGGAGCGTTTTGTAGGCTCTCCAGGGNGTTG
AGATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCA
TAGGCAGAGGAACCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAG
ATGAATGGGCCCAATCCAAAGCCACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATG
CGGAGTTAAAGGAGGAGTTCACTCGGATTTTGACAGCCAACAACCCACACGCACCCCANA
ACATTGTCAGGTACAGCTTCAAAAAGCAATGGAGTCTGGGTTTCATTCCTAAACTGAAGA
GGAAGATCCCAGTCNACTACTGGGGACAAGATGAAGGTGAAATCTCCACTGAAACTGTAA
GGGTAATTTATTCCTTGGGAATCTTCTTCTCAAAATATTTCCTCTTTTTTTTGGACCTTT
TTTTTGANCCTTTTTCTTGTCGCATCTCTGGGGTTACTTGGTCCTGTTTTTGTTTTCCTT
GCCTTTCTTGCCCTCCTTNNNCTCCCCCCCCCCCCGCCCTCCTGGCGTCTTTGTNTTTTT
CTTTTTTTCTTCTTGTTTCTCCCCCCCCCCCTTGTTGCCCCCCCTCTTTTCCGGCTGCCC
CCCGCCCGGTGTTNTTCCGCTCGCGTTCCCCCCCCCGCCCGCNTCTTTTCTGCCGCCGTC
TTTNGTGGTTTATTTGTTGTTNGGNNGTGTGTGGCGNGTCGACGGATCGAAAAAAAAAAA
>OriGene 3' read for NM_012144 unedited
CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGTTTCTACACAGGTTTATTTGT
GCAAAGTTAGAGGGGTAATGGTGGTTGAAGACCAAGTCCTGGGGTAAGGTGCTGGGACCT
TAAGGAACAGAACAAGAAGGGAAGATGCTGGGACCTGATCAGGAGTGGGGTCACATGCTG
GGTGCTAAGGCTGGGCTGGGTACCAGGGTCAAGCCCTAGGAGTACTGTATTCAAGCGATG
GGACAGAGACTGAGGCCAGCCCCTCAGGTCTTGATTTTCACTTCCCTCACCAGGTTCAGC
AGTTTGTCCAGTTTCGCAATCTCCACAGCTGGACCCTTCTGCACCTTCTGCCCCTTCTTT
TCCTTTGGCATCTTGCGCAAATTGGGTGAGAGCTTGAGGCTGATGATGTGCCCACGGTCA
TCGCCCACAATGATGATGGGGTGGATGAGATTGAACTGCACGTGGGTGAGCCTGTTCTTT
TTGCCCGCACAGGCTGGTTGCAGATGGGCTCATACTTGCTGATGGCTAAGTACATATGTG
GGCCTTCCCATTTGGGTGACTGCTGTGAACACAGTAAAGACATGGCGCCCAGCCACATTA
CCCAGGGTGAGTTACGCCATAGATAACCTCCGGTTTTGATGTTTCGGCCACATCTTATTG
TCCACTCGACCCGAGACTTGACACCTTGTGTGTTGGGTTTTAGAACCCGGCTCACTGACG
TTTGGGCCGTTCACGGGTCGCACCCCCCGTGCTTGATTTAAATCCCGCCCCCTGCGCCGC
CGTTGTAAAATTGGTTATTCCCTCAGCGCCCTTATACGCCCTCTCTCGTCCCTTTTCTTC
CTTCCCTTCCTTTTTTCCCCTTTCTACCCCCTTTTTCCTCCCCCCTCCCCCCTTTTCCCC
CTCTCCTTTTCCTTCCCCCCCTTCCCCTCCCCCCCACTTTCTTCATTTTCTCTCCCTCTC
CTCTCCCTCTTCCCCCACCTCTTCTCCC
Restriction Sites NotI-NotI     
ACCN NM_012144
Insert Size 3520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012144.2, NP_036276.1
RefSeq Size 2548 bp
RefSeq ORF 2100 bp
Locus ID 27019
UniProt ID Q9UI46
Domains WD40
Protein Families Druggable Genome
Protein Pathways Huntington's disease
Gene Summary This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcript Variant: This variant (1) encodes isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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