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Gemin 8 (GEMIN8) (NM_001042480) Human Tagged ORF Clone
CAT＃: RC204795
GEMIN8 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1
ORF Plasmid: tGFP
"NM_001042480" in other vectors (5)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FAM51A1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204795 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGTAAAGGCATCAACATCGAAAGCTACCAGGCCTTGGTATTCTCATCCGGTATATGCAAGAT ACTGGCAACATTATCATCAAGCAATGGCTTGGATGCAAAGCCATCACAATGCCTACAGGAAGGCCGTGGA ATCCTGTTTCAATCTTCCATGGTACTTACCTTCTGCGCTTCTTCCCCAAAGCTCTTACGATAATGAGGCT GCGTATCCTCAGTCCTTCTATGACCATCATGTGGCCTGGCAGGACTACCCCTGCAGTTCTTCACATTTCA GAAGATCTGGGCAGCATCCACGTTACAGCAGTAGGATCCAGGCATCCACAAAAGAAGACCAAGCTTTGTC CAAAGAGGAAGAGATGGAGACTGAGTCAGATGCAGAGGTAGAATGTGACCTGAGCAATATGGAAATCACT GAAGAGCTCCGCCAGTACTTTGCAGAGACCGAGAGGCATAGAGAAGAACGACGGCGGCAGCAGCAGCTGG ATGCAGAGCGCCTGGACAGCTATGTGAACGCTGACCACGACCTGTACTGCAACACCCGCCGGTCGGTAGA AGCCCCAACTGAGAGGCCTGGTGAGCGGCGCCAGGCCGAGATGAAGCGTTTGTACGGGGACAGTGCTGCC AAGATCCAAGCCATGGAGGCCGCGGTGCAGCTGAGCTTTGACAAGCACTGTGACCGAAAGCAGCCCAAGT ACTGGCCGGTCATCCCCCTGAAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204795 protein sequence
Red=Cloning site Green=Tags(s) MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEA AYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEIT EELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAA KIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042480 |
| ORF Size | 726 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042480.2 |
| RefSeq Size | 3089 bp |
| RefSeq ORF | 729 bp |
| Locus ID | 54960 |
| UniProt ID | Q9NWZ8 |
| MW | 28.6 kDa |
| Gene Summary | The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204795L3 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204795L4 | Lenti ORF clone of Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204795 | GEMIN8 (tGFP-tagged) - Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1 |
CNY 4,000.00 |
|
| SC311242 | GEMIN8 (untagged)-Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1 |
CNY 3,990.00 |
|
| SC320956 | GEMIN8 (untagged)-Human gem (nuclear organelle) associated protein 8 (GEMIN8), transcript variant 1 |
CNY 2,400.00 |
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