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Troponin C (TNNC2) (NM_003279) Human Tagged ORF Clone
CAT＃: RC202754
TNNC2 (Myc-DDK-tagged)-Human troponin C type 2 (fast) (TNNC2)
ORF Plasmid: tGFP
"NM_003279" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CFAP85; FAP85 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202754 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACCAGCAGGCTGAGGCCAGGTCCTACCTCAGCGAAGAGATGATCGCTGAGTTCAAGGCTGCCT TTGACATGTTTGATGCTGATGGTGGTGGGGACATCAGCGTCAAGGAGTTGGGCACGGTGATGAGGATGCT GGGCCAGACACCCACCAAGGAGGAGCTGGACGCCATCATCGAGGAGGTGGATGAGGACGGCAGCGGCACC ATCGACTTCGAGGAGTTCTTGGTCATGATGGTGCGCCAGATGAAAGAGGACGCGAAAGGGAAGAGCGAGG AGGAGCTGGCCGAGTGCTTCCGCATCTTCGACAGGAATGCAGACGGCTACATCGACCCGGAGGAGCTGGC TGAGATTTTCAGGGCCTCCGGGGAGCACGTGACTGACGAGGAGATCGAATCTCTGATGAAAGACGGCGAC AAGAACAACGACGGCCGCATTGACTTCGACGAGTTCCTGAAGATGATGGAGGGCGTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202754 protein sequence
Red=Cloning site Green=Tags(s) MTDQQAEARSYLSEEMIAEFKAAFDMFDADGGGDISVKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGT IDFEEFLVMMVRQMKEDAKGKSEEELAECFRIFDRNADGYIDPEELAEIFRASGEHVTDEEIESLMKDGD KNNDGRIDFDEFLKMMEGVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003279 |
| ORF Size | 480 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003279.1 |
| RefSeq Size | 698 bp |
| RefSeq ORF | 483 bp |
| Locus ID | 7125 |
| UniProt ID | P02585 |
| Domains | EFh |
| Protein Pathways | Calcium signaling pathway |
| MW | 18.1 kDa |
| Gene Summary | Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202754L1 | Lenti ORF clone of Human troponin C type 2 (fast) (TNNC2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC202754L2 | Lenti ORF clone of Human troponin C type 2 (fast) (TNNC2), mGFP tagged |
CNY 5,890.00 |
|
| RC202754L3 | Lenti ORF clone of Human troponin C type 2 (fast) (TNNC2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202754L4 | Lenti ORF clone of Human troponin C type 2 (fast) (TNNC2), mGFP tagged |
CNY 5,890.00 |
|
| RG202754 | TNNC2 (tGFP-tagged) - Human troponin C type 2 (fast) (TNNC2) |
CNY 2,800.00 |
|
| SC118101 | TNNC2 (untagged)-Human troponin C type 2 (fast) (TNNC2) |
CNY 1,200.00 |
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