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STT3A (NM_152713) Human Tagged ORF Clone
CAT＃: RC201991
STT3A (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A)
ORF Plasmid: tGFP
"NM_152713" in other vectors (6)
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CNY 5,168.00
CNY 5,800.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ITM1; STT3-A; TMC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201991 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAAGTTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTTTGAAGCTTCTCATTCTGT CAATGGCTGCTGTATTATCCTTCTCCACTCGTCTGTTTGCTGTCCTGAGATTTGAAAGTGTTATCCATGA GTTTGATCCGTACTTTAATTATCGGACTACCAGGTTCCTGGCTGAGGAGGGGTTTTATAAATTCCATAAC TGGTTTGATGACCGAGCCTGGTACCCTTTGGGACGAATCATTGGAGGAACAATTTACCCAGGTTTAATGA TCACCTCTGCTGCAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCTGTGT GTTCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAAGGAT GCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGATCTGTGGCTG GCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTACTACATGTGGATCAAGGCAGT AAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTTGCTTATTTCTACATGGTCTCGTCATGGGGA GGTTATGTGTTCCTGATCAACTTAATTCCTCTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTCTCTC ACCGGATCTATGTGGCCTACTGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCTTTGT GGGTTTCCAGCCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGATCCAT GCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGGAGCGTCATCT CTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACAGGAAAAATATCTCCCTGGAC GGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATCCCCATCATTGCTTCTGTGTCT GAGCATCAGCCCACAACCTGGTCCTCATACTATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCAGTTG GCCTCTATTACTGCTTTAGCAACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCAGCAT GTACTTTTCAGCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGGCATT GGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAGAAGAGCAAGA AGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATGATACTGGTCATGGCTTTCTT TCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGTGAGGCCTACTCTTCTCCGTCCATTGTACTA TCTGCCCGTGGTGGGGATGGCAGTAGGATCATATTTGATGACTTCCGAGAAGCATATTATTGGCTTCGTC ATAATACTCCAGAGGATGCGAAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGGCAAA CCGAACAATTTTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCG TCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTCATTTTTGGAG GCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTCCGGATTGGAGGGAGCACAGA TACAGGCAAACATATCAAGGAGAATGACTATTATACTCCAACTGGGGAGTTCCGTGTGGACCGTGAAGGT TCTCCAGTGCTGCTCAACTGCCTCATGTACAAGATGTGTTACTATCGCTTTGGACAGGTTTACACAGAAG CCAAGCGTCCTCCAGGCTTTGACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTGATGT CCTGGAGGAAGCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA GGCTTGTCAAGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201991 protein sequence
Red=Cloning site Green=Tags(s) MTKFGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHN WFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKD AGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSICWAAKCALAYFYMVSSWG GYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIH AFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVS EHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGI GVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVL SARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMA STEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTDTGKHIKENDYYTPTGEFRVDREG SPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR GLSRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152713 |
| ORF Size | 2115 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152713.5 |
| RefSeq Size | 4244 bp |
| RefSeq ORF | 2118 bp |
| Locus ID | 3703 |
| UniProt ID | P46977 |
| Domains | STT3 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 80.5 kDa |
| Gene Summary | The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex
,null,
mBio
,PubMed ID 28720733
[STT3A]
|
|
Dengue virus hijacks a noncanonical oxidoreductase function of a cellular oligosaccharyltransferase complex
,Lin, DL;Cherepanova, NA;Bozzacco, L;Macdonald, MR;Gilmore, R;Tai, AW;,
bioRxiv
[STT3A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201991L1 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), Myc-DDK-tagged |
CNY 7,568.00 |
|
| RC201991L2 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), mGFP tagged |
CNY 7,700.00 |
|
| RC201991L3 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201991L4 | Lenti ORF clone of Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A), mGFP tagged |
CNY 7,700.00 |
|
| RG201991 | STT3A (tGFP-tagged) - Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A) |
CNY 6,370.00 |
|
| SC127610 | STT3A (untagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (STT3A) |
CNY 2,950.00 |
