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S4 (PSMC1) (NM_002802) Human Tagged ORF Clone
CAT＃: RC201810
PSMC1 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1)
ORF Plasmid: tGFP
"NM_002802" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P26S4; p56; RPT2; S4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201810 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCAAAGTCAGAGTGGTGGTCATGGTCCTGGAGGTGGCAAGAAGGATGACAAGGACAAGAAAAAGA AATATGAACCTCCTGTACCAACTAGAGTGGGGAAAAAGAAGAAGAAAACAAAGGGACCAGATGCTGCCAG CAAACTGCCACTGGTGACACCTCACACTCAGTGCCGGTTAAAATTACTGAAGTTAGAGAGAATTAAAGAC TATCTTCTCATGGAGGAAGAATTCATTAGAAATCAGGAACAAATGAAACCATTAGAAGAAAAGCAAGAGG AGGAAAGATCAAAAGTGGATGATCTGAGGGGGACCCCGATGTCAGTAGGAACCTTGGAAGAGATTATTGA TGACAATCATGCCATCGTGTCTACATCTGTGGGCTCAGAACACTACGTCAGCATTCTTTCATTTGTAGAC AAGGATCTGCTGGAACCTGGCTGCTCGGTCCTGCTCAACCACAAGGTGCATGCCGTGATAGGGGTGCTGA TGGATGACACGGATCCCCTGGTCACAGTGATGAAGGTAGAAAAGGCCCCCCAGGAGACCTATGCAGATAT TGGGGGGTTGGACAACCAAATTCAGGAAATTAAGGAATCTGTGGAGCTTCCTCTCACCCATCCTGAATAT TATGAAGAGATGGGTATAAAGCCTCCTAAGGGGGTCATTCTCTATGGTCCACCTGGCACAGGTAAAACCT TGTTAGCCAAAGCAGTAGCAAACCAAACCTCAGCCACTTTCTTGAGAGTGGTTGGCTCTGAACTTATTCA GAAGTACCTAGGTGATGGGCCCAAACTCGTACGGGAATTGTTCCGAGTTGCTGAAGAACATGCACCGTCC ATCGTGTTTATTGATGAAATTGACGCCATTGGGACAAAAAGATATGACTCCAATTCTGGTGGTGAGAGAG AAATTCAGCGAACAATGTTGGAACTGCTGAACCAGTTGGATGGATTTGATTCTAGGGGAGATGTGAAAGT TATCATGGCCACAAACCGAATAGAAACTTTGGATCCAGCACTTATCAGACCAGGCCGCATTGACAGGAAG ATTGAGTTCCCCCTGCCTGATGAAAAGACGAAGAAGCGCATCTTTCAGATTCACACAAGCAGGATGACGC TGGCTGATGATGTAACCCTGGACGACCTGATCATGGCTAAAGATGACCTCTCTGGTGCTGACATCAAGGC AATCTGTACAGAAGCTGGTCTGATGGCCTTAAGAGAACGTAGAATGAAAGTAACAAATGAAGACTTCAAA AAATCTAAAGAAAATGTTCTTTATAAGAAACAGGAAGGCACCCCTGAGGGGCTGTATCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201810 protein sequence
Red=Cloning site Green=Tags(s) MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKD YLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVD KDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEY YEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPS IVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRK IEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFK KSKENVLYKKQEGTPEGLYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002802 |
| ORF Size | 1320 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002802.3 |
| RefSeq Size | 1586 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 5700 |
| UniProt ID | P62191 |
| Domains | AAA, AAA |
| Protein Pathways | Proteasome |
| MW | 49.2 kDa |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201810L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201810L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1), mGFP tagged |
CNY 5,890.00 |
|
| RC201810L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201810L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1), mGFP tagged |
CNY 5,890.00 |
|
| RG201810 | PSMC1 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1) |
CNY 4,370.00 |
|
| SC321319 | PSMC1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 1 (PSMC1) |
CNY 3,656.00 |
