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LAPTM4A (NM_014713) Human Tagged ORF Clone
CAT＃: RC201290
LAPTM4A (Myc-DDK-tagged)-Human lysosomal protein transmembrane 4 alpha (LAPTM4A)
ORF Plasmid: tGFP
"NM_014713" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HUMORF13; LAPTM4; MBNT; Mtrp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201290 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCATGAGTTTCAAGCGGAACCGCAGTGACCGGTTCTACAGCACCCGGTGCTGCGGCTGTTGCC ATGTCCGCACCGGGACGATCATCCTGGGGACCTGGTACATGGTAGTAAACCTATTGATGGCAATTTTGCT GACTGTGGAAGTGACTCATCCAAACTCCATGCCAGCTGTCAACATTCAGTATGAAGTCATCGGTAATTAC TATTCTTCTGAGAGAATGGCTGATAATGCCTGTGTTCTTTTTGCCGTCTCTGTTCTTATGTTTATAATCA GTTCAATGCTGGTTTATGGATCAATTTCTTATCAAGTGGGTTGGCTGATTCCATTCTTCTGTTACCGACT TTTTGACTTCGTCCTCAGTTGCCTGGTTGCTATTAGTTCTCTCACCTATTTGCCAAGAATCAAAGAATAT CTGGATCAACTACCTGATTTTCCCTACAAAGATGACCTCCTGGCCTTGGACTCCAGCTGCCTCCTGTTCA TTGTTCTTGTGTTCTTTGCCTTATTCATCATTTTTAAGGCTTATCTAATTAACTGTGTTTGGAACTGCTA TAAATACATCAACAACCGAAACGTGCCGGAGATTGCTGTGTACCCTGCCTTTGAAGCACCTCCTCAGTAC GTTTTGCCAACCTATGAAATGGCCGTGAAAATGCCTGAAAAAGAACCACCACCTCCTTACTTACCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201290 protein sequence
Red=Cloning site Green=Tags(s) MVSMSFKRNRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNY YSSERMADNACVLFAVSVLMFIISSMLVYGSISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEY LDQLPDFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFEAPPQY VLPTYEMAVKMPEKEPPPPYLPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014713 |
| ORF Size | 699 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014713.5 |
| RefSeq Size | 1783 bp |
| RefSeq ORF | 702 bp |
| Locus ID | 9741 |
| UniProt ID | Q15012 |
| Domains | Mtp |
| Protein Families | Transmembrane |
| Protein Pathways | Lysosome |
| MW | 26.8 kDa |
| Gene Summary | This gene encodes a protein that has four predicted transmembrane domains. The function of this gene has not yet been determined; however, studies in the mouse homolog suggest a role in the transport of small molecules across endosomal and lysosomal membranes. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Vpr counteracts the restriction of LAPTM5 to promote HIV-1 infection in macrophages
,Zhao, L;Wang, S;Xu, M;He, Y;Zhang, X;Xiong, Y;Sun, H;Ding, H;Geng, W;Shang, H;Liang, G;,
Nature communications
,PubMed ID 34140527
[LAPTM4A]
|
|
CRISPR screen reveals that EHEC's T3SS and Shiga toxin rely on shared host factors for infection
,Pacheco, A;Lazarus, J;Sit, B;Schmieder, S;Lencer, W;Blondel, C;Doench, J;Davis, B;Waldor, M;,
bioRxiv
[LAPTM4A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201290L1 | Lenti ORF clone of Human lysosomal protein transmembrane 4 alpha (LAPTM4A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201290L2 | Lenti ORF clone of Human lysosomal protein transmembrane 4 alpha (LAPTM4A), mGFP tagged |
CNY 5,890.00 |
|
| RC201290L3 | Lenti ORF clone of Human lysosomal protein transmembrane 4 alpha (LAPTM4A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201290L4 | Lenti ORF clone of Human lysosomal protein transmembrane 4 alpha (LAPTM4A), mGFP tagged |
CNY 4,800.00 |
|
| RG201290 | LAPTM4A (tGFP-tagged) - Human lysosomal protein transmembrane 4 alpha (LAPTM4A) |
CNY 4,000.00 |
|
| SC321578 | LAPTM4A (untagged)-Human lysosomal protein transmembrane 4 alpha (LAPTM4A) |
CNY 2,400.00 |
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