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MAN2B1 (NM_000528) Human Tagged ORF Clone
CAT＃: RC200638
MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1
ORF Plasmid: tGFP
"NM_000528" in other vectors (6)
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CNY 7,040.00
CNY 7,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LAMAN; MANB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200638 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGCCCCTGGACCA TGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTGTTGCTGGCGGCTGCCGGTGC TCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCGAACATGCTGAACGTGCACCTGCTGCCTCAC ACACATGATGACGTGGGCTGGCTCAAAACCGTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGC ACGCCGGTGTGCAGTACATCCTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCAT TTACGTGGAGATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAGGCAGCCACCC ACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAGGACACATTTGGCAATGATGG GCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCACTCTCGGGAGCAGGCCTCGCTGTTTGCGCAG ATGGGCTTCGACGGCTTCTTCTTTGGGCGCCTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGG AGATGGAGCAGGTGTGGCGGGCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCT TCCCAATGGTTACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCCACTGCCCAGG GCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTCCAATATGAGAATGCCAACAT GTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAATGCGCAGCAGGCAAAAGGAAGCAGTGTCCAT GTTCTCTACTCCACCCCCGCTTGTTACCTCTGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAAC ATGACGACTTCTTCCCTTACGCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGC CCTCAAACGCTACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGC CTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATGGCTGTGCTCC AGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGACTACGCGCGCCAGCTTGCGGC AGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTGGCGCGGCTCAGAGGCTTCAAAGATCACTTC ACCTTTTGCCAACAGCTAAACATCAGCATCTGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCG TTTATAATCCCCTGGGGCGGAAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGT GAAGGACCCCAATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCAC CCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTAGCCCAGGTGC CTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCCTGGTCCCCTGCTTTAACCAT CGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACAGGGCTGTTGATGGAGATTATGAACATGAAT CAGCAACTCCTGCTGCCTGTTCGCCAGACCTTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTG ACCAGGCCTCAGGTGCCTACATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCA GATCCACCTGGTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTG GTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCTGTGGGCGACA CCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAGGGACGCTTCTACACAGACAG CAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGACCCACCTGGAAACTGAACCAGACGGAGCCC GTGGCAGGAAACTACTATCCAGTCAACACCCGGATTTACATCACGGATGGAAACATGCAGCTGACTGTGC TGACTGACCGCTCCCAGGGGGGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCT GCTGAAGGACGATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGG CGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCGGAGCAGGAGG TCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAATCTCGGGGCTCCTCCGCGCAC GCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTGCACCTGCTCACGCTGGCCAGCTGGGGCCCC GAAATGGTGCTGCTGCGCTTGGAGCACCAGTTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCC CCGTTACCTTGAACTTGAGGGACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGT GGCCAACCAGCTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAA ACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTCCTGGCCTCAG TTCAATGGAAGGAGGTGGATGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200638 protein sequence
Red=Cloning site Green=Tags(s) MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPH THDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVR DLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQ MGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVE DPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVH VLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHF TFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAH PPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMN QQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQV VRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEP VAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGP EMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQ TPYQLDPANITLEPMEIRTFLASVQWKEVDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000528 |
| ORF Size | 3033 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000528.4 |
| RefSeq Size | 3231 bp |
| RefSeq ORF | 3036 bp |
| Locus ID | 4125 |
| UniProt ID | O00754 |
| Domains | Glyco_hydro_38 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Other glycan degradation |
| MW | 113.7 kDa |
| Gene Summary | This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Engineering of GlcNAc-1-Phosphotransferase for Production of Highly Phosphorylated Lysosomal Enzymes for Enzyme Replacement Therapy
,Liu, L;Lee, W;Doray, B;Kornfeld, S;,
Molecular Therapy-Methods & Clinical Development
,PubMed ID 28480305
[MAN2B1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200638L1 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, Myc-DDK-tagged |
CNY 9,440.00 |
|
| RC200638L2 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RC200638L3 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC200638L4 | Lenti ORF clone of Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RG200638 | MAN2B1 (tGFP-tagged) - Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1 |
CNY 9,500.00 |
|
| SC319430 | MAN2B1 (untagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1 |
CNY 7,048.00 |
