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RNase H1 (RNASEH1) (NM_002936) Human Tagged ORF Clone
CAT＃: RC200595
RNASEH1 (Myc-DDK-tagged)-Human ribonuclease H1 (RNASEH1)
ORF Plasmid: tGFP
"NM_002936" in other vectors (6)
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CNY 2,400.00
CNY 4,465.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | H1RNA; PEOB2; RNH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200595 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGGCTTCTGTTCCTGGCCCACAGAGTCGCCTTGGCCGCCTTGCCCTGCCGCCGCGGCTCTCGCG GGTTCGGGATGTTCTATGCCGTGAGGAGGGGCCGCAAGACCGGGGTCTTTCTGACCTGGAATGAGTGCAG AGCACAGGTGGACCGGTTTCCTGCTGCCAGATTTAAGAAGTTTGCCACAGAGGATGAGGCCTGGGCCTTT GTCAGGAAATCTGCAAGCCCGGAAGTTTCAGAAGGGCATGAAAATCAACATGGACAAGAATCGGAGGCGA AAGCCAGCAAGCGACTCCGTGAGCCACTGGATGGAGATGGACATGAAAGCGCAGAGCCGTATGCAAAGCA CATGAAGCCGAGCGTGGAGCCGGCGCCTCCAGTTAGCAGAGACACGTTTTCCTACATGGGAGACTTCGTC GTCGTCTACACTGATGGCTGCTGCTCCAGTAATGGGCGTAGAAGGCCGCGAGCAGGAATCGGCGTTTACT GGGGGCCAGGCCATCCTTTAAATGTAGGCATTAGACTTCCTGGGCGGCAGACAAACCAAAGAGCGGAAAT TCATGCAGCCTGCAAAGCCATTGAACAAGCAAAGACTCAAAACATCAATAAACTGGTTCTGTATACAGAC AGTATGTTTACGATAAATGGTATAACTAACTGGGTTCAAGGTTGGAAGAAAAATGGGTGGAAGACAAGTG CAGGGAAAGAGGTGATCAACAAAGAGGACTTTGTGGCACTGGAGAGGCTTACCCAGGGGATGGACATTCA GTGGATGCATGTTCCTGGTCATTCGGGATTTATAGGCAATGAAGAAGCTGACAGATTAGCCAGAGAAGGA GCTAAACAATCGGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200595 protein sequence
Red=Cloning site Green=Tags(s) MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAF VRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFV VVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTD SMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREG AKQSED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002936 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002936.6 |
| RefSeq Size | 1865 bp |
| RefSeq ORF | 861 bp |
| Locus ID | 246243 |
| UniProt ID | O60930 |
| Domains | rnaseH |
| Protein Pathways | DNA replication |
| MW | 32.1 kDa |
| Gene Summary | This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1
,Vickers, TA;Rahdar, M;Prakash, TP;Crooke, ST;,
Nucleic Acids Res.
,PubMed ID 31495875
[RNase H1]
|
|
TRF1 participates in chromosome end protection by averting TRF2-dependent telomeric R loops
,Lee, YW;Arora, R;Wischnewski, H;Azzalin, CM;,
Nat. Struct. Mol. Biol.
,PubMed ID 29358759
[RNase H1]
|
|
Dynamic nucleoplasmic and nucleolar localization of mammalian RNase H1 in response to RNAP I transcriptional R-loops
,Shen, W;Sun, H;De Hoyos, CL;Bailey, JK;Liang, X;Crooke, ST;,
Nucleic Acids Research
,PubMed ID 28977560
[RNase H1]
|
|
SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage
,Jangi, M;Fleet, C;Cullen, P;Gupta, SV;Mekhoubad, S;Chiao, E;Allaire, N;Bennett, CF;Rigo, F;Krainer, AR;Hurt, JA;Carulli, JP;Staropoli, JF;,
Proc. Natl. Acad. Sci. U.S.A
,PubMed ID 28270613
[RNase H1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200595L1 | Lenti ORF clone of Human ribonuclease H1 (RNASEH1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200595L2 | Lenti ORF clone of Human ribonuclease H1 (RNASEH1), mGFP tagged |
CNY 4,800.00 |
|
| RC200595L3 | Lenti ORF clone of Human ribonuclease H1 (RNASEH1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200595L4 | Lenti ORF clone of Human ribonuclease H1 (RNASEH1), mGFP tagged |
CNY 4,800.00 |
|
| RG200595 | RNASEH1 (tGFP-tagged) - Human ribonuclease H1 (RNASEH1) |
CNY 4,000.00 |
|
| SC319446 | RNASEH1 (untagged)-Human ribonuclease H1 (RNASEH1) |
CNY 2,400.00 |
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