Arhgap27 (NM_001205236) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310069I04Rik; 5730442P18Rik; Gm21552; Sh3d20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231292 representing NM_001205236 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGACGTCGAGGGGGACGTGTACGTGCTGGTGGAACACCCCTTCGAGTACACCGGCAAAGACG GGCGCCGCATCGCCATCCAACCGAACGAGCGTTACCGACTGCTGCGCCGCAGCACCGAGCACTGGTGGCA CGTGCGGCGGGAGCCCGGGGGTCGCCCCTTCTACCTCCCCGCGCAGTACGTACGCGAGCTGCCCGCGCTC GGCGACCCTGCCCCGGCCCCGCAGCCTTCCGTACCGCAGCAGCGCCCCGCAGTCCCGGAACCTCTGGCCT ACGACTACCGCTTCGTGAGCACCCCGGTGGGCGCCGATGGGTCCTCTGCCGAGCCCCGGGGCCGCGCCAG CTCCCTGTGTGGCCCTGCACGACAGCGCACCGGTGGCCAGCGCAACAGCCTGGCTCCGGGAGGGCCCGCC TGCCTTTACGTGCGGCCCGCGGCGCCTGTGCGACCGGCGCAGTCCCTGGATGACTTGGCGCGCGGCGGTA CCGCGCCCCCTGCCGGCCTCCTCGGCAGCGCGGGCCACTTCAAGGCCTCCAGCGTGGCGGGCTCCTGGGT TTGTCCCCGGCCCCTGGCGCCTAGCGACTCGGAGAATGTCTACGAGGCCATTCCGGACTTGCGCTGCCCT CCGCGGGCAGAGAGCCCAAAGCAGGTGGACGACCCCCCGGAACCAGTGTATGCCAATGTAGAGAGGCAAC CTCGGGCCACGTCGCCTCGCTCAGCTGCAGCTCCTCCTCGGCTCAGCCCAGTGTGGGAGACACACACAGA CACGGGCACTGGGCGCCCCTACTACTACAACCCCGACACGGGCGTGACCACCTGGGAGTCACCCTTTGAA ACACCTGAAGGCACCACCAGCCCGGCCACCTCCCGGGCCTCTGTGGGTAGCGGGGAGAGCCTGGAGACAG AGTGGGGCCAGTACTGGGATGAGGAGAGCCGCAGGGTCTTCTTCTACAACCCCTTGACCGGTGAGACGGC CTGGGAGGACGAGACCGAGGAGCTGGAGGAAGATCACCAGGAACAACTGGAAATGCAACCCAGCCTGAGC CCGCGAAGCCCCGGGCAGCAGAGGCCCCCAACTCCTGAAACAGACTACCCCGAATTACTGGCCAGTTACC CTGAAGAGGACTACTCTCCTGTGGGCTCCTTCAGTGATCCGGGCCCCGCTTCTCCTTTGGTTGCACCCCC TGGCTGGTCCTGTCAAATTACCCCAGACAAACAGATGCTCTACACCAACCAGTTCACTCAAGAGCAGTGG GTAAGGTTGGAGGACCAGCACGGGAAGCCATATTTCTACAACCCAGAGGACTCCTCTGTTCAGTGGGAGC TACCCCAGGTCCCGATCCCTGCCCCCCGAAGTGTCCGCAAATCCAGCCAGGATAGCGACACTCCAGCCCA GGCTAGCCCTCCAGAGGAGAAGATCAAGACTCTGGACAAGGCAGGAGTCCTCCATCGTACCAAGACGGTA GACAAGGGGAAGAGACTTCGGAAGAAACACTGGAGCACCTCCTGGACAGTGTTGGAGGGCGGGGTCCTGA CCTTCTTCAAGGACTCAAAGACCTCAGCTGCAGGCGGCCTGAGGCAGCCTTCCAAGCTCTCCACCCCTGA ATACACAGTCGAACTGAAGGGGGCCTCTCTCTCTTGGGCCCCCAAAGACAAATCCAGCAAGAAGAATGTG TTAGAGCTTCGAAGCCGAGATGGCTCGGAGTACCTGATCCAGCACGACTCCGAAGCCATCATCAGCACCT GGCACAAAGCCATTGCCGAAGGCATCTCAGAGCTGTCTGCAGACCTGCTCCAAGGGGAGGAAGGTGAGCC CAGCAGTGCTGATTTCGGGTCCAGTGAGCGCCTCGGAAGCTGGCGGGAGGAGGATGTACGGCAGAACGCA GCCTCACCCTCGCTCAGCCCCGGAGGCCTGGAAAGCGACTTGAGCAGAGTCCGGCACAAGCTCCGTAAAT TCCTACAGAGACGACCCACTCTGCAGTCTTTGCGGGACAAGGGCTACATCAAAGACCAGGTGTTTGGATG CGCGCTGGCTCAACTGTGTGAGCGCGAGAGGAGCCCTGTGCCACGCTTCGTGCAGCAATGCATCCGCACC GTCGAGGCCCGGGGGCTGGATATTGATGGGCTGTACCGCATCAGTGGGAACCTGGCTACCATCCAGAAGC TGCGCTATAAGGTGGATCACGATGAACGCTTGGACTTGGACGACGGGCGCTGGGAAGACGTCCACGTGAT CACGGGTGCCCTGAAGCTCTTCTTCCGAGAGCTGCCGGAACCTCTCTTCCCCTTCTCGCACTTCCACCAG TTCATAGCAGCCATTAAGCTGCAGGACCCAGCCCAGCGCAGCCGCTGCGTGCGTGACCTGGTGCGCACGC TGCCAGCTCCCAACCAGGACACGCTCCGACTGCTCATCCAGCATCTCTGCCGGGTCATCGAGCATGGCGA GCAGAACCGTATGACTGTGCAGAATGTGGCCATAGTGTTCGGACCCACGCTGCTGCGGCCAGAGATGGAG GAGGCCAGCATGCCCATGACCATGGTGTTCCAGAACCAGGTGGTGGAGCTCATCCTACATCAGTGCGCGG ACATCTTCCCACCGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231292 representing NM_001205236 Red=Cloning site Green=Tags(s) MAADVEGDVYVLVEHPFEYTGKDGRRIAIQPNERYRLLRRSTEHWWHVRREPGGRPFYLPAQYVRELPAL GDPAPAPQPSVPQQRPAVPEPLAYDYRFVSTPVGADGSSAEPRGRASSLCGPARQRTGGQRNSLAPGGPA CLYVRPAAPVRPAQSLDDLARGGTAPPAGLLGSAGHFKASSVAGSWVCPRPLAPSDSENVYEAIPDLRCP PRAESPKQVDDPPEPVYANVERQPRATSPRSAAAPPRLSPVWETHTDTGTGRPYYYNPDTGVTTWESPFE TPEGTTSPATSRASVGSGESLETEWGQYWDEESRRVFFYNPLTGETAWEDETEELEEDHQEQLEMQPSLS PRSPGQQRPPTPETDYPELLASYPEEDYSPVGSFSDPGPASPLVAPPGWSCQITPDKQMLYTNQFTQEQW VRLEDQHGKPYFYNPEDSSVQWELPQVPIPAPRSVRKSSQDSDTPAQASPPEEKIKTLDKAGVLHRTKTV DKGKRLRKKHWSTSWTVLEGGVLTFFKDSKTSAAGGLRQPSKLSTPEYTVELKGASLSWAPKDKSSKKNV LELRSRDGSEYLIQHDSEAIISTWHKAIAEGISELSADLLQGEEGEPSSADFGSSERLGSWREEDVRQNA ASPSLSPGGLESDLSRVRHKLRKFLQRRPTLQSLRDKGYIKDQVFGCALAQLCERERSPVPRFVQQCIRT VEARGLDIDGLYRISGNLATIQKLRYKVDHDERLDLDDGRWEDVHVITGALKLFFRELPEPLFPFSHFHQ FIAAIKLQDPAQRSRCVRDLVRTLPAPNQDTLRLLIQHLCRVIEHGEQNRMTVQNVAIVFGPTLLRPEME EASMPMTMVFQNQVVELILHQCADIFPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205236
ORF Size	2607 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205236.1, NP_001192165.1
RefSeq Size	4559 bp
RefSeq ORF	2610 bp
Locus ID	544817
UniProt ID	A2AB59
MW	97.5 kDa
Gene Summary	Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]

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