Trim9 (NM_001286388) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231187 representing NM_001286388 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGATGGAAGAAGAGTTAAAATGCCCTGTGTGTGGCTCCTTCTATCGGGAGCCCATCATCTTGC CCTGCTCTCACAATTTATGTCAGGCGTGCGCCCGCAACATCCTGGTGCAGACCCCGGAGTCCGAGTCCCC CCAGAGCCGCCGGGCCTCGGGCTCTGGGGTTTCTGACTATGATTATCTGGACCTGGACAAGATGAGCCTG TACAGCGAGGCGGACAGCGGCTATGGCTCCTACGGAGGTTTCGCCAGCGCCCCCACTACCCCGTGCCAGA AGTCGCCCAACGGCGTCCGCGTTTTCCCCCCTGCTATGCCGCCACCGCCCACCCACCTGTCACCGGCTTT GGCCCCTGTGCCCCGCAATTCCTGCATCACCTGCCCCCAGTGCCACCGCAGCCTCATTCTGGATGACCGG GGGCTCCGCGGTTTCCCCAAGAACCGCGTCCTGGAAGGGGTCATCGACCGCTACCAACAGAGCAAAGCCG CGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACGGTCATGTGTGAACAGTGCGATGT CTTCTACTGCGACCCTTGCCGTCTGCGCTGCCACCCGCCCCGGGGTCCCCTAGCCAAACACCGTCTGGTG CCCCCGGCCCAGGGCCGCGTCAGCCGGCGGCTGAGCCCGCGCAAGGTCTCCACCTGCACAGACCACGAGC TGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTGTGCTACCAGTGCCTAGAGGAGGGCAA ACACTCCAGCCACGAAGTCAAGGCTTTGGGGGCTATGTGGAAATTGCACAAGAGCCAGCTCTCCCAGGCC CTGAATGGATTGTCGGACAGGGCCAAAGAAGCCAAGGAGTTCCTGGTGCAGCTCCGCACCATGGTACAAC AGATCCAGGAAAACAGTGTGGAGTTTGAGGCCTGCCTGGTGGCCCAGTGTGATGCGCTCATCGATGCCCT AAACCGAAGGAAGGCTCAGCTGCTGGCCCGGGTCAACAAGGAGCATGAACACAAGCTGAAGGTGGTTCGG GACCAGATCTCTCACTGCACGGTGAAGCTGCGTCAGACCACGGGCCTCATGGAGTACTGCTTGGAAGTGA TTAAGGAGAACGACCCCAGTGGCTTTTTGCAGATTTCAGATGCTCTCATAAGGCGAGTTCACTTAACTGA GGACCAGTGGGGGAAGGGCACCCTCACTCCCAGGATGACCACAGACTTTGATCTGAGCCTGGACAACAGC CCTCTGCTGCAGTCTATTCACCAACTGGACTTCGTGCAAGTGAAAGCCTCCTCTCCAGTCCCAGCAACCC CCATCCTACAGCTGGAGGAGTGTTGCACCCACAACAACAGTGCTACGCTGTCCTGGAAACAGCCCCCTCT GTCCACCGTAGCCGCCGATGGATACATCCTGGAGCTGGATGACGGCAGTGGTGGTCAGTTCCGGGAAGTG TATGTTGGAAAAGAAACAATGTGCACAGTGGACGGCCTTCACTTCAACAGCACATACAACGCTCGGGTTA AGGCCTTCAACAAAACAGGAGTCAGCCCCTACAGCAAGACACTGGTCCTCCAGACGTCTGAGGCAGCGGG AGCCCATGAGACAAAACCTATGAAGGACACAGACTCAGAAGAACAGACCCTCCCCTTTCCAGTGCCTTCG GAAAGATTGCCGCTCCGTAGGATGAGCCCTTTCTCCTCCACCCTTAATCTACAACCCAGCTTCCCCGGGA GGTCCTACTTTGATTTCCGGTCCTCACCCCACCAGCTGAGTTTGCATTCTTCTTTACAGTCTCTCAATGC ACCGGGCTGCAATTTTGAGACACAATCTGCATCCTACTCTCAATTAGTTGACATTAAAAAGCTGTTGGCA GTGGCTTGGTTTGCTTTTGACCCCGGCTCGGCCCACTCCGACATCATCTTCTCCAATGACAACTTGACAG TGACGTGCAGTAGCTACGATGACCGGGTGGTGCTGGGGAAAACCGGCTTCTCCAAAGGCGTCCACTACTG GGAGCTGACCATAGATCGCTATGACAACCACCCTGATCCAGCATTTGGCGTGGCTCGCATCGATGTGATG AAGGATATGATGTTAGGGAAGGACGACAAAGCTTGGGCAATGTATGTGGACAATAACCGGAGCTGGTTCA TGCACAACAACTCGCACACCAACAGAACTGAAGGAGGGATCACAAAAGGGGCCACCATCGGGGTCCTCCT CGACTTAAACAGAAAGACATTAACGTTTTTCGTCAACAATGAACAGCAAGGCCCCATAGCGTTTGAGAAC GTGGAGGGCCTGTTCTTCCCTGCTGTCAGCCTGAACAGGAATGTGCAGGTCACGCTCCACACCGGGCTTC CGGTCCCCGACTTCTACTCCAGCCGAGCTTCCATAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231187 representing NM_001286388 Red=Cloning site Green=Tags(s) MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSRRASGSGVSDYDYLDLDKMSL YSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPPPTHLSPALAPVPRNSCITCPQCHRSLILDDR GLRGFPKNRVLEGVIDRYQQSKAAALKCQLCEKAPKEATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLV PPAQGRVSRRLSPRKVSTCTDHELENHSMYCVQCKMPVCYQCLEEGKHSSHEVKALGAMWKLHKSQLSQA LNGLSDRAKEAKEFLVQLRTMVQQIQENSVEFEACLVAQCDALIDALNRRKAQLLARVNKEHEHKLKVVR DQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHLTEDQWGKGTLTPRMTTDFDLSLDNS PLLQSIHQLDFVQVKASSPVPATPILQLEECCTHNNSATLSWKQPPLSTVAADGYILELDDGSGGQFREV YVGKETMCTVDGLHFNSTYNARVKAFNKTGVSPYSKTLVLQTSEAAGAHETKPMKDTDSEEQTLPFPVPS ERLPLRRMSPFSSTLNLQPSFPGRSYFDFRSSPHQLSLHSSLQSLNAPGCNFETQSASYSQLVDIKKLLA VAWFAFDPGSAHSDIIFSNDNLTVTCSSYDDRVVLGKTGFSKGVHYWELTIDRYDNHPDPAFGVARIDVM KDMMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTEGGITKGATIGVLLDLNRKTLTFFVNNEQQGPIAFEN VEGLFFPAVSLNRNVQVTLHTGLPVPDFYSSRASIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286388
ORF Size	2418 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286388.1, NP_001273317.1
RefSeq Size	4734 bp
RefSeq ORF	2421 bp
Locus ID	94090
UniProt ID	Q8C7M3
MW	90.3 kDa
Gene Summary	E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.[UniProtKB/Swiss-Prot Function]

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