Cysltr1 (NM_001281862) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BB147369; Cyslt1; CysLT1R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229339 representing NM_001281862 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAACTGAAAATCTGACGACATCTCTCATTAATAACACGTGTCATGACACAATTGATGAATTCC GAAATCAAGTATACTCCACTATGTATTCTGTGATCTCTGTTGTGGGTTTCTTTGGCAATAGCTTTGTGCT CTATGTCCTCATAAAAACATACCATGAGAAATCAGCCTTCCAAGTATACATGATTAATCTAGCCATAGCA GATCTACTCTGTGTATGTACATTGCCTCTCCGTGTGGTCTATTATGTTCACAAAGGCAAGTGGCTCTTTG GTGACTTTTTGTGCCGCCTCACCACCTATGCCTTGTACGTTAACCTCTATTGTAGCATCTTCTTTATGAC AGCCATGAGCTTTTTCCGGTGTGTTGCAATTGTCTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAA AAAGCCAGGTTCGTTTGCATTGGAATTTGGATTTTTGTGATTTTGACAAGTTCTCCCTTTTTAATGTACA AATCTTACCAAGATGAGAAAAACAATACTAAATGCTTTGAGCCTCCACAGAACAATCAAGCTAAAAAATA CGTTTTGATCTTGCATTATGTGTCATTATTCTTTGGTTTCATCATCCCTTTTGTCACCATAATTGTCTGT TACACAATGATCATTCTGACCTTACTAAAAAATACAATGAAGAAAAACATGCCAAGTCGTAGGAAGGCTA TAGGGATGATCATAGTTGTGACAGCTGCCTTTTTAGTGAGCTTCATGCCATATCATATTCAACGAACTAT CCACCTTCACCTTTTACACAGTGAAACTAGACCCTGTGATTCTGTCCTTAGGATGCAGAAGTCAGTGGTC ATAACCTTATCTCTAGCTGCATCAAATTGTTGCTTTGATCCTCTGCTATATTTCTTTTCAGGTGGAAACT TTAGGAGAAGGCTATCTACATTTAGAAAGCATTCTTTGTCCAGTATGACTTATGTACCCAAGAAGAAAGC TTCCTTGCCAGAAAAAGGAGAAGAAATATGTAACGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229339 representing NM_001281862 Red=Cloning site Green=Tags(s) MNGTENLTTSLINNTCHDTIDEFRNQVYSTMYSVISVVGFFGNSFVLYVLIKTYHEKSAFQVYMINLAIA DLLCVCTLPLRVVYYVHKGKWLFGDFLCRLTTYALYVNLYCSIFFMTAMSFFRCVAIVFPVQNINLVTQK KARFVCIGIWIFVILTSSPFLMYKSYQDEKNNTKCFEPPQNNQAKKYVLILHYVSLFFGFIIPFVTIIVC YTMIILTLLKNTMKKNMPSRRKAIGMIIVVTAAFLVSFMPYHIQRTIHLHLLHSETRPCDSVLRMQKSVV ITLSLAASNCCFDPLLYFFSGGNFRRRLSTFRKHSLSSMTYVPKKKASLPEKGEEICNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281862
ORF Size	1017 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281862.1, NP_001268791.1
RefSeq Size	2802 bp
RefSeq ORF	1020 bp
Locus ID	58861
UniProt ID	Q99JA4
MW	39.6 kDa
Gene Summary	Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.[UniProtKB/Swiss-Prot Function]

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