Ms4a2 (NM_001276330) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Fce1b; Fcer1; Fcer1b; fcERI; FcR; FcRB; Fcrbe; Fcrbeta; Ms4a1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228485 representing NM_001276330 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACAGAAAATAGGAGCAGAGCAGATCTTGCTCTCCCAAATCCACAAGAATCCTCCAGTGCACCTG ACATTGAACTCTTGGAAGCATCTCCTGCCAAAGCAGCCCCACCAAAGCAGACATGGCGGACATTTTTGAA GAAAGAGTTGGAGTTCCTGGGAGCAACACAAATTCTGGTTGGTTTGATATGCCTTTGTTTTGGAACAATT GTCTGCTCCGTACTCTATGTTTCAGACTTTGATGAAGAAGTGCTTTTACTTTATAAACTAGGCTATCCAT TCTGGGGTGCAGTGCTGTTTGTTTTGTCTGGATTTTTGTCAATTATCTCCGAAAGAAAAAACACATTGTA TCTGAGACACCGTCCTCCCTTTGGAATCTCCATCTCTAAAATAGAGTTCTGTACTAAGGAGGAACTGGTG TTGATGATGCTGTTTCTCACCATCCTGGCCTTTTGCAGTGCTGTGTTGTTCACTATCTATAGGATTGGAC AAGAGTTAGAAAGTAAAAAGGTCCCAGATGATCGTCTTTATGAAGAATTAAATGTGTATTCACCAATTTA CAGTGAGTTGGAAGACAAAGGGGAAACATCTTCTCCAGTTGATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228485 representing NM_001276330 Red=Cloning site Green=Tags(s) MDTENRSRADLALPNPQESSSAPDIELLEASPAKAAPPKQTWRTFLKKELEFLGATQILVGLICLCFGTI VCSVLYVSDFDEEVLLLYKLGYPFWGAVLFVLSGFLSIISERKNTLYLRHRPPFGISISKIEFCTKEELV LMMLFLTILAFCSAVLFTIYRIGQELESKKVPDDRLYEELNVYSPIYSELEDKGETSSPVDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276330
ORF Size	606 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276330.1, NP_001263259.1
RefSeq Size	2660 bp
RefSeq ORF	609 bp
Locus ID	14126
MW	23.3 kDa
Gene Summary	This gene encodes a member of the membrane-spanning 4A family. The encoded protein is the beta subunit of the high affinity IgE receptor and is localized to the membrane. The encoded protein is required for full activation of mast cells, including the release of histamine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

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