Tet1 (NM_027384) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2510010B09Rik; AA517754; BB001228; Cxxc6; D10Ertd17e; LCX; mKIAA1676
Vector	pCMV6-Entry2
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227558 representing NM_027384 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGTCCCGCCCCGCAAAGCCTTCCAAATCAGTCAAAACAAAGCTACAGAAAAAGAAAGACATCC AGATGAAGACGAAGACATCAAAGCAGGCTGTCAGACATGGGGCATCAGCGAAGGCAGTGAACCCCGGAAA ACCGAAGCAATTAATTAAAAGAAGAGATGGTAAGAAGGAAACGGAAGACAAAACCCCGACACCGGCGCCG AGTTTTCTGACGCGAGCCGGGGCCGCCCGAATGAATCGGGATCGAAACCAAGTTCTTTTTCAGAACCCAG ACTCCTTAACTTGCAACGGGTTTACAATGGCTCTTCGAAGAACCTCTCTTAGCTGGCGACTCTCCCAGCG CCCAGTAGTCACACCCAAACCTAAGAAAGTACCACCTTCAAAGAAGCAGTGTACACATAATATCCAGGAC GAGCCGGGGGTAAAGCACTCTGAAAATGATTCAGTCCCCAGCCAACACGCTACCGTGTCCCCAGGCACAG AGAATGGTGAACAGAACAGGTGTTTAGTTGAAGGGGAGAGCCAAGAGATAACCCAGTCTTGCCCTGTATT TGAGGAAAGAATAGAAGATACCCAAAGCTGTATTTCCGCATCTGGGAACCTGGAAGCAGAAATTTCTTGG CCACTGGAAGGGACACATTGTGAAGAACTACTTTCTCATCAGACATCTGATAATGAGTGCACTTCCCCTC AAGAGTGTGCTCCATTGCCCCAAAGATCAACTTCCGAAGTTACCTCTCAGAAAAACACTAGCAATCAGTT AGCAGACTTGAGCTCACAAGTAGAGTCTATCAAGCTGTCTGATCCTTCTCCGAATCCTACGGGAAGCGAC CATAATGGCTTTCCAGACTCCAGTTTTAGGATCGTTCCTGAATTGGACCTGAAAACCTGCATGCCTCTTG ATGAGTCTGTGTATCCCACAGCTCTGATAAGATTCATCTTGGCAGGCTCACAGCCGGATGTCTTTGATAC TAAGCCCCAAGAAAAAACCCTTATAACAACCCCAGAACAAGTGGGAAGCCACCCAAATCAAGTTCTGGAT GCCACCTCTGTCCTAGGACAGGCCTTTAGTACTCTTCCACTTCAGTGGGGATTTTCTGGTGCTAACTTAG TTCAAGTTGAGGCCCTGGGCAAGGGTTCAGACTCACCAGAGGATCTTGGTGCTATTACTATGCTAAACCA ACAAGAAACTGTGGCTATGGATATGGATAGGAATGCTACCCCGGACCTGCCTATCTTCCTTCCTAAGCCT CCAAACACAGTTGCTACCTACAGTAGCCCTCTTTTAGGGCCTGAGCCCCACAGCTCTACCTCCTGTGGGC TTGAAGTCCAAGGTGCTACCCCGATTTTAACTTTGGACTCAGGACACACTCCCCAACTCCCACCAAACCC TGAGTCAAGTTCAGTACCTCTGGTAATAGCTGCAAACGGTACGCGAGCTGAGAAGCAGTTTGGTACCAGC CTTTTCCCAGCCGTCCCACAAGGGTTTACAGTCGCAGCCGAGAATGAAGTCCAGCATGCCCCATTAGATC TTACCCAGGGTTCTCAGGCTGCTCCCAGCAAACTGGAAGGAGAGATTTCTCGGGTCAGCATCACTGGCTC AGCTGATGTCAAAGCCACAGCGATGTCCATGCCGGTTACACAGGCCAGTACCTCTTCTCCCCCATGTAAT TCTACGCCGCCAATGGTGGAGAGGAGGAAGCGCAAGGCCTGTGGGGTCTGTGAGCCGTGCCAGCAGAAGG CCAACTGTGGGGAGTGCACCTACTGCAAGAATCGAAAGAACAGCCACCAGATCTGTAAGAAGAGGAAATG CGAGGTGCTCAAAAAGAAGCCAGAGGCCACCTCACAGGCACAGGTTACAAAAGAAAACAAGAGGCCCCAG AGGGAAAAGAAGCCCAAAGTTTTAAAGACAGACTTTAACAACAAACCAGTAAATGGCCCCAAATCAGAGT CCATGGACTGCAGTAGACGTGGTCATGGGGAAGAAGAGCAAAGGTTGGATTTGATCACACATCCCCTTGA AAATGTAAGGAAGAATGCAGGCGGCATGACGGGAATTGAGGTGGAGAAGTGGGCGCCAAACAAGAAATCA CACTTAGCAGAGGGTCAAGTCAAAGGAAGCTGTGATGCCAATTTAACTGGGGTGGAAAACCCCCAGCCCT CTGAAGATGACAAGCAGCAAACCAACCCTTCTCCGACATTTGCCCAGACCATAAGGAACGGCATGAAAAA TGTACACTGTTTACCCACTGACACACACCTTCCGCTCAACAAATTGAATCATGAGGAATTTAGCAAGGCG TTGGGAAATAATTCCTCTAAATTGCTGACTGATCCCTCAAACTGCAAGGATGCCATGAGTGTCACCACCT CCGGGGGGGAGTGTGATCATCTCAAGGGGCCAAGGAACACCCTGCTCTTCCAGAAGCCTGGCTTGAACTG CAGGTCTGGTGCAGAACCCACCATCTTTAATAATCACCCTAATACACACAGCGCTGGGAGTCGACCTCAC CCTCCTGAGAAGGTACCCAACAAAGAACCCAAAGATGGCTCTCCGGTTCAGCCAAGTCTTCTATCCCTAA TGAAGGACAGGAGATTAACGCTGGAACAAGTGGTAGCCATAGAAGCCCTGACTCAGCTCTCAGAAGCGCC CTCAGAGAGCTCCTCCCCATCCAAGCCGGAGAAGGATGAAGAAGCGCATCAGAAAACGGCTAGTCTGCTT AACAGTTGCAAGGCTATCCTTCATTCGGTGAGAAAAGACCTCCAAGACCCAAACGTTCAAGGAAAGGGCC TTCACCATGACACTGTTGTCTTTAATGGCCAGAACAGAACATTCAAGTCACCTGATAGTTTCGCTACTAA CCAAGCACTTATAAAATCACAAGGATATCCAAGTTCACCTACAGCTGAGAAGAAAGGCGCTGCTGGTGGG AGAGCTCCCTTTGATGGTTTTGAGAATTCACATCCATTGCCCATAGAAAGTCACAATCTTGAAAACTGCA GTCAGGTACTAAGCTGCGATCAGAATTTGAGTTCCCATGACCCATCATGCCAGGATGCACCCTACAGTCA AATTGAAGAAGATGTCGCAGCACAATTGACCCAACTTGCATCCACGATTAATCACATCAACGCCGAGGTC AGGAATGCTGAAAGTACACCAGAAAGCCTTGTTGCAAAGAACACAAAGCAAAAACACAGTCAGGAGAAAC GCATGGTACATCAGAAGCCACCCTCTAGTACACAAACTAAACCCAGTGTACCATCAGCAAAGCCAAAGAA GGCCCAGAAAAAGGCAAGAGCGACCCCACACGCAAACAAGCGGAAAAAGAAACCCCCAGCCCGAAGCTCT CAAGAAAATGATCAGAAGAAGCAAGAGCAGCTAGCTATAGAGTATAGTAAAATGCATGACATTTGGATGT CATCTAAGTTTCAAAGGTTTGGCCAGTCTAGTCCACGTAGTTTTCCTGTTCTGTTGAGAAATATTCCTGT CTTTAACCAAATATTAAAGCCCGTGACTCAGAGTAAAACACCCTCACAACATAATGAGTTATTTCCTCCT ATCAATCAGATAAAATTCACAAGAAACCCTGAATTGGCAAAGGAGAAAGTGAAGGTTGAACCATCAGATT CTCTGCCAACATGCCAATTCAAGACAGAATCCGGTGGGCAGACATTTGCAGAGCCGGCAGATAATTCTCA GGGACAGCCCATGGTGAGTGTCAATCAGGAAGCTCACCCTCTGCCCCAGTCCCCACCATCTAACCAGTGT GCTAATATAATGGCTGGTGCTGCCCAAACACAGTTTCATCTAGGCGCTCAAGAGAACCTGGTGCATCAGA TACCACCACCGACGTTGCCTGGTACCTCCCCTGACACACTCTTGCCAGACCCAGCATCAATCCTCAGGAA AGGGAAAGTGTTACATTTCGATGGAATTACAGTTGTTACGGAGAAGCGTGAAGCTCAAACATCAAGCAAT GGACCACTGGGTCCCACCACAGACAGTGCACAGTCAGAATTTAAAGAGAGCATCATGGACTTACTTAGCA AGCCTGCAAAAAACCTGATAGCTGGATTGAAGGAACAGGAAGCTGCACCCTGTGACTGTGATGGAGGTAC ACAAAAAGAAAAAGGCCCATATTATACACACCTTGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTC ATGGAGACTAGGTTTGGCCAGAAGGGGAAGGCAATCCGGATTGAGAAGATAGTGTTCACGGGGAAGGAAG GGAAGAGCTCTCAGGGCTGCCCGGTCGCCAAGTGGGTGATCAGAAGAAGTGGTCCTGAAGAGAAGCTTAT TTGTTTGGTTCGTGAGCGTGTAGACCATCACTGTTCGACGGCTGTGATAGTTGTCCTTATCCTGCTGTGG GAAGGTATCCCTCGCCTGATGGCTGACCGCCTGTACAAAGAGCTCACTGAGAACTTGAGGTCCTACAGCG GACATCCCACAGACCGAAGATGTACCCTCAACAAAAAGCGTACCTGCACCTGTCAAGGCATCGACCCAAA AACCTGCGGAGCGTCCTTCTCCTTTGGCTGTTCGTGGAGCATGTATTTCAACGGCTGTAAGTTTGGGAGG AGTGAAAACCCCAGAAAATTCAGACTTGCTCCAAACTACCCCTTACATGAAAAACAGCTTGAAAAGAATT TACAAGAATTGGCTACAGTATTAGCTCCACTTTACAAGCAGATGGCTCCAGTTGCTTATCAAAATCAGGT GGAATATGAAGAAGTTGCTGGAGACTGTCGACTTGGAAATGAAGAGGGGCGTCCTTTCTCTGGTGTCACC TGTTGCATGGATTTTTGTGCCCATTCTCACAAGGACATTCACAACATGCACAACGGAAGCACCGTGGTGT GTACGTTGATTCGAGCAGATGGCCGTGACACAAATTGTCCCGAGGATGAACAACTCCACGTCCTGCCACT ATACCGGCTTGCAGACACTGATGAATTTGGCTCCGTGGAAGGGATGAAGGCCAAAATCAAATCTGGGGCC ATCCAAGTCAATGGGCCAACCAGGAAGAGGCGACTACGTTTTACTGAGCCTGTTCCTCGATGTGGGAAGA GGGCCAAAATGAAGCAGAACCACAATAAATCAGGTTCACACAACACTAAGAGCTTTTCATCAGCCTCATC TACTTCTCACCTAGTGAAAGACGAATCTACAGACTTCTGTCCCCTGCAGGCTTCCTCCGCAGAAACATCT ACCTGTACGTACAGTAAAACAGCCTCAGGTGGGTTTGCAGAAACAAGTAGTATTCTCCACTGCACAATGC CTTCTGGAGCACACAGTGGTGCTAATGCAGCTGCTGGGGAATGTACTGGAACGGTGCAGCCTGCCGAGGT GGCTGCTCATCCTCACCAGTCTCTTCCCACAGCCGATTCTCCCGTTCATGCTGAGCCTCTCACTAGTCCA TCTGAGCAGCTAACTTCTAACCAGTCAAACCAGCAGCTCCCTCTCCTCAGCAATTCTCAGAAACTGGCTT CCTGTCAGGTGGAAGATGAGCGGCACCCTGAAGCGGATGAGCCTCAGCACCCCGAGGACGATAACTTGCC TCAACTTGATGAATTCTGGTCAGACAGTGAGGAGATCTACGCCGATCCTTCCTTTGGTGGCGTGGCGATA GCACCCATTCACGGCTCGGTGCTCATTGAGTGCGCTCGGAAGGAGCTTCATGCTACCACCTCTTTGCGCT CCCCCAAACGAGGGGTCCCTTTTCGTGTGTCCCTTGTATTCTACCAGCACAAAAGCCTAAACAAGCCTAA TCATGGTTTTGATATCAACAAAATTAAGTGTAAATGCAAAAAAGTAACGAAAAAAAAGCCCGCAGACCGG GAGTGTCCTGATGTATCCCCCGAAGCCAATTTATCACACCAAATTCCTTCTCGAGTTGCATCAACCTTAA CCCGAGACAATGTTGTTACCGTGTCCCCATACTCTCTCACTCATGTTGCGGGACCCTACAATCGTTGGGT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227558 representing NM_027384 Red=Cloning site Green=Tags(s) MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAP SFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQD EPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISW PLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSD HNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLD ATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKP PNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTS LFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCN STPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQ REKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKS HLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKA LGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPH PPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLL NSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGG RAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEV RNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSS QENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPP INQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQC ANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSN GPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVREL METRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLW EGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGR SENPRKFRLAPNYPLHEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVT CCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGA IQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETS TCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSP SEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAI APIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHKSLNKPNHGFDINKIKCKCKKVTKKKPADR ECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTHVAGPYNRWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_027384
ORF Size	6021 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_027384.1, NP_081660.1
RefSeq Size	13986 bp
RefSeq ORF	6024 bp
Locus ID	52463
UniProt ID	Q3URK3
MW	219.7 kDa
Gene Summary	Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).[UniProtKB/Swiss-Prot Function]

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