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Gnas (NM_201617) Mouse Tagged ORF Clone
CAT＃: MR225835
- TrueORF®
Gnas (Myc-DDK-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6
ORF Plasmid: tGFP
"NM_201617" in other vectors (4)
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CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5530400H20Rik; A930027G11Rik; C130027O20Rik; G; Ga; Galphas; Gn; Gnas1; Gnasxl; GPSA; Gs-; Gs-alpha; Gsa; GSP; N; Nes; Nesp; Nesp55; Nespl; Oed; Oed-Sml; Oedsml; P; P1; P2; P3; PHP1A; PHP1B; POH; SCG; SCG6; XL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225835 representing NM_201617
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGTTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGGG AGCAGCCCGAGCAAGAACCTTTGGAAGCCCCAGGGGCAGCTGCCCCCGGTGCTGGGGCTGGCCCAGCCGA AGAAATGGCGACCGAACCGGACTCCGAACCGTCTAACAATGAGCCCGTCCCCGACGAGACTGGCAGTGAG ATCAGTGGACCCCCAGAAGACTCCAAATCTGACATCCAAAGCCCCTGCCAGGCCTTCGAGGAAGTCCGAG TGGGTGGAGACTACAGCCCACCTCCGGAGGAAGCCATGCCATTCGAGACACAACAGCCCAGCCTGGGAGA TTTCTGGCCCACCCTGGAGCAGCCAGGACCATCTGGGACCCCATCAGGCCTCCAAGCCTTCAACCCAGCG ATTTTGGAGCCCGGGACCCCCACTGGCGCGAGCCCAGGCCTGGGAGCCTATACCCCCCCACCAGAAGAAG CTATGCCATTTGAGTTCAACGAGCCTGCCCAGGGAGACCATAGCCAGCCTCCCTTGCAAGTCCCAGACCT TGCGCCAGGAGGTCCGGAAGCATTGGTCCCCAGAGCTCTTCCCGCGGAGCCCGGGAACATCAGATTTGAA AACGCTGGCTTCCGAGAAGACTACAGCCCTCCCCCTGAAGAATCTGTGCCATTTCAGGTCGGTGGAGAAG AATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGAGTCATCCCACAAATCGGCATTGGCGGCGAGTT CCCGACAGTCGCGGTCCCGAGTGCGCTCTGCCTCGCTCCCGCCGAGAACGCGCCTCCCCTCTGGGTCCGA GGCGCCATTGACAGACCATTCCGCGAGGCTGTCAGATCTCCTCCTAACTTCGCATGCGACAGCCCCCCGA TGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCCATTGGGGTCGACGACGACACCGCTGTCAA TATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCCCCAGATAAGAGCGAG TGCGCAGAGAGACCCCCAGTTGAGCGAGAAGCAGCCGAGATGGAAGGAAGCCCTACCACCGCCACTGCGG TGGAAGGAAAAGTCCCCTCTCCGGAGAGAGGGGACGGATCTTCCACCCAGCCTGAAGCAATGGATGCCAA GCCAGCCCCTGCTGCCCAAGCCGTCTCTACCGGATCTGATGCTGGAGCTCCTACGGATTCCGCGATGCTC ACAGATAGCCAGAGCGATGCCGGAGAAGACGGGACAGCCCCAGGAACGCCTTCAGATCTCCAGTCGGATC CTGAAGAACTCGAAGAAGCCCCAGCTGTCCGCGCCGATCCTGACGGAGGGGCAGCCCCAGTCGCCCCAGC CACTCCTGCCGAGTCCGAGTCTGAAGGCAGCAGAGATCCAGCCGCCGAGCCAGCCTCCGAGGCAGTCCCT GCCACCACGGCCGAGTCTGCCTCCGGGGCAGCCCCTGTCACCCAGGTGGAGCCCGCAGCCGCGGCAGTCT CTGCCACCCTGGCGGAGCCTGCCGCCCGGGCAGCCCCTATCACCCCCAAGGAGCCCACTACCCGGGCAGT CCCCTCTGCTAGAGCCCATCCGGCCGCTGGAGCAGTCCCTGGCGCCCCAGCAATGTCAGCCTCTGCTAGG GCAGCTGCCGCTAGGGCAGCCTATGCAGGTCCACTGGTCTGGGGAGCCAGGTCACTCTCAGCTACTCCCG CCGCTCGGGCATCCCTTCCTGCCCGCGCAGCAGCTGCCGCCCGGGCAGCCTCTGCTGCCCGCGCAGTCGC TGCTGGCCGGTCAGCCTCTGCCGCCCCCAGCAGGGCCCATCTTAGACCCCCCAGCCCCGAGATCCAGGTT GCTGACCCGCCTACTCCGCGGCCTCCTCCGCGGCCGACTGCCTGGCCTGACAAGTACGAGCGGGGCCGAA GCTGCTGCAGGTACGAGGCATCGTCTGGCATCTGCGAGATCGAGTCCTCCAGTGATGAGTCGGAAGAAGG GGCCACCGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGCCGCCCTGGCCTGCCCCGGAGCCACACGGTC GGGAGCAACCCAGTCCGCAACTTCTTCACCCGAGCCTTCGGAAGCTGCTTCGGTCTATCCGAGTGTACCC GATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCTATGGAGGAGAGGCGCAAACAGATGCGCAAAGA AGCCATTGAGATGCGAGAGCAGAAGCGCGCAGATAAGAAACGCAGCAAGCTCATCGACAAGCAACTGGAG GAGGAGAAGATGGACTACATGTGTACACACCGCCTGCTGCTTCTAGGGAGAAAAGTGGTGCCGAGCGACA CTGAGGGTCGTTACCGGCCGGAGGCCAGCGCCAGCGCCAGCGATCGTAGGCTGGACAGGCGGGGGCGTGA GGTGAGCCCAGAACTGCTGGGGTGGGCCCTTCGGGGCTCCCCCGGCTCGATTGTTCGTGACCGCGGTGGG CTAGGGCCATCCGGGTGCGCGCCCCCGCCTCGCCTGGCACGGCTGCTTCGACTCAGACAGCTTGTTGTTG GTGTGTGTTGGTGTCCATTTTCTGTGTTCGCCTGTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225835 representing NM_201617
Red=Cloning site Green=Tags(s) MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSE ISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPGPSGTPSGLQAFNPA ILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQPPLQVPDLAPGGPEALVPRALPAEPGNIRFE NAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVR GAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSE CAERPPVEREAAEMEGSPTTATAVEGKVPSPERGDGSSTQPEAMDAKPAPAAQAVSTGSDAGAPTDSAML TDSQSDAGEDGTAPGTPSDLQSDPEELEEAPAVRADPDGGAAPVAPATPAESESEGSRDPAAEPASEAVP ATTAESASGAAPVTQVEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASAR AAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQV ADPPTPRPPPRPTAWPDKYERGRSCCRYEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTV GSNPVRNFFTRAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLE EEKMDYMCTHRLLLLGRKVVPSDTEGRYRPEASASASDRRLDRRGREVSPELLGWALRGSPGSIVRDRGG LGPSGCAPPPRLARLLRLRQLVVGVCWCPFSVFACA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201617 |
| ORF Size | 2628 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201617.2, NP_963911.1 |
| RefSeq Size | 2731 bp |
| RefSeq ORF | 2631 bp |
| Locus ID | 14683 |
| UniProt ID | Q6R0H7 |
| MW | 91.5 kDa |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222309 | Gnas (untagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6, (10ug) |
CNY 11,210.00 |
|
| MG225835 | Gnas (tGFP-tagged) - Mouse GNAS (guanine nucleotide binding protein alpha stimulating) complex locus (Gnas) transcript variant 6, (10ug) |
CNY 9,600.00 |
|
| MR225835L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6 |
CNY 10,640.00 |
|
| MR225835L4 | Lenti ORF clone of Gnas (mGFP-tagged) - Mouse GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus (Gnas), transcript variant 6 |
CNY 10,640.00 |
