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Pik3cd (NM_008840) Mouse Tagged ORF Clone
CAT＃: MR225827
- TrueORF®
Pik3cd (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 4
ORF Plasmid: tGFP
"NM_008840" in other vectors (4)
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CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410099E07Rik; 2610208K16Rik; AW545373; p110delta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225827 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCTGGGGTGGACTGCCCCATGGAGTTCTGGACCAAAGAGGAGAGCCAGAGCGTGGTTGTTGACT TCTTGCTGCCCACAGGGGTCTACTTGAACTTCCCCGTGTCCCGCAATGCCAACCTCAGCACCATCAAGCA GGTGCTGTGGCACCGTGCACAGTATGAGCCACTCTTCCACATGCTCAGTGACCCCGAGGCCTATGTGTTC ACCTGTGTGAACCAGACGGCGGAGCAGCAGGAGTTGGAGGATGAGCAGCGGAGGCTGTGCGACATCCAGC CCTTCCTGCCCGTGCTGCGCCTCGTGGCCCGAGAGGGGGACCGCGTGAAGAAGCTCATTAACTCCCAGAT CAGCCTCCTCATTGGCAAAGGTCTCCATGAGTTTGATTCCCTGCGGGACCCGGAAGTAAACGACTTCCGC ACTAAGATGCGCCAGTTTTGTGAAGAGGCTGCTGCTCACCGCCAGCAGCTGGGCTGGGTGGAATGGCTGC AGTACAGCTTCCCCCTGCAGCTGGAGCCCTCAGCAAGGGGTTGGCGGGCCGGCTTATTGCGTGTCAGCAA CCGAGCCCTGCTGGTCAACGTGAAGTTCGAGGGCAGTGAGGAGAGCTTCACCTTCCAGGTATCCACCAAG GACATGCCCCTGGCACTGATGGCCTGTGCCCTCCGAAAAAAGGCCACAGTGTTCCGGCAGCCTCTGGTGG AGCAGCCTGAGGAATATGCCCTGCAGGTGAACGGGAGGCACGAATACCTCTACGGCAACTACCCGCTCTG CCACTTTCAGTACATCTGCAGCTGCCTACACAGCGGGCTGACCCCTCATCTGACCATGGTCCACTCCTCC TCCATCCTTGCTATGCGGGATGAGCAGAGCAATCCTGCCCCCCAAGTACAGAAACCACGTGCCAAACCTC CCCCGATCCCTGCCAAGAAGCCCTCCTCTGTGTCCCTGTGGTCCCTGGAACAGCCATTCTCCATTGAGCT GATCGAGGGCCGAAAAGTGAATGCTGACGAGCGGATGAAGCTGGTTGTTCAGGCCGGGCTCTTCCATGGC AATGAGATGCTGTGCAAGACTGTGTCAAGCTCGGAGGTGAATGTATGCTCAGAGCCCGTGTGGAAGCAGC GACTGGAGTTCGATATCAGCGTCTGTGACCTCCCGCGCATGGCTCGACTCTGTTTTGCTCTCTATGCCGT CGTGGAGAAGGCTAAGAAGGCACGCTCCACAAAGAAGAAGTCTAAGAAGGCGGACTGCCCCATCGCTTGG GCCAACCTCATGCTATTCGACTACAAAGATCAGCTCAAGACGGGGGAGCGCTGCCTCTACATGTGGCCCT CTGTCCCAGATGAGAAGGGAGAGCTGCTGAATCCTGCGGGTACAGTGCGCGGGAACCCCAACACGGAGAG TGCCGCTGCCCTGGTCATCTACCTGCCTGAGGTGGCCCCCCACCCTGTGTACTTCCCCGCTCTGGAGAAG ATCCTGGAGCTGGGGCGTCACGGGGAGCGTGGGCGCATCACGGAGGAGGAGCAGCTGCAGCTGCGGGAGA TCCTGGAACGGCGGGGATCCGGGGAACTGTACGAACATGAGAAGGACCTGGTGTGGAAGATGCGCCACGA AGTCCAGGAGCATTTCCCAGAGGCGCTGGCCCGCCTGCTGCTGGTCACCAAGTGGAATAAACACGAGGAT GTGGCCCAGATGCTCTATTTGCTGTGCTCCTGGCCCGAGCTGCCTGTGCTGAGCGCCCTGGAACTTCTGG ACTTTAGCTTTCCCGACTGCTACGTGGGCTCCTTCGCCATCAAGTCCCTTCGGAAGCTGACGGACGATGA GCTCTTCCAGTACCTTCTGCAGCTGGTGCAAGTGCTCAAATATGAGTCCTACCTGGACTGCGAGCTGACC AAATTCTTGCTGGGCCGAGCCCTGGCTAACCGCAAGATCGGACACTTCCTGTTCTGGCACCTCCGCTCTG AGATGCACGTACCATCAGTGGCTCTGCGGTTTGGTCTCATCATGGAAGCCTACTGCAGAGGCAGCACCCA CCACATGAAGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAGCTTAAGGCACTGAATGACTTTGTGAAG GTGAGTTCCCAGAAGACCACCAAGCCCCAAACCAAGGAGATGATGCATATGTGCATGCGCCAGGAGACCT ACATGGAGGCCCTGTCCCACCTGCAGTCTCCACTCGACCCCAGCACCCTGCTGGAGGAAGTCTGTGTGGA GCAGTGCACCTTCATGGACTCCAAAATGAAGCCCCTGTGGATCATGTACAGCAGCGAGGAGGCGGGCAGT GCTGGCAACGTGGGCATCATCTTTAAGAACGGGGATGACCTCCGCCAGGACATGCTGACTCTGCAGATGA TCCAGCTCATGGACGTCCTGTGGAAGCAGGAGGGCCTGGACCTGAGGATGACGCCCTACGGCTGCCTCCC CACCGGGGACCGCACAGGTCTCATCGAGGTGGTCCTCCACTCGGACACCATCGCCAACATCCAGCTGAAC AAAAGCAACATGGCGGCCACAGCTGCCTTCAACAAGGACGCCCTGCTCAACTGGCTCAAGTCCAAGAACC CTGGGGAGGCCCTGGATCGGGCCATTGAGGAATTCACCCTCTCCTGTGCTGGCTACTGTGTGGCCACATA TGTTCTGGGCATCGGTGACCGGCACAGCGACAACATCATGATCAGAGAGAGTGGGCAGCTCTTCCACATT GATTTTGGCCACTTTCTGGGGAACTTCAAGACCAAGTTTGGAATCAACCGAGAGCGCGTCCCCTTCATTC TCACCTACGACTTTGTCCACGTGATCCAGCAGGGGAAGACTAACAACAGTGAGAAGTTTGAAAGGTTCCG CGGCTACTGTGAACGAGCCTATACCATCCTGCGGCGCCACGGGCTGCTTTTCCTCCATCTCTTCGCCCTG ATGCGGGCCGCAGGTCTGCCTGAGCTTAGCTGCTCCAAAGATATCCAGTATCTCAAGGACTCTCTGGCAC TGGGGAAGACGGAGGAAGAGGCGCTAAAGCACTTCCGGGTGAAGTTCAACGAAGCTCTCCGAGAAAGCTG GAAAACCAAAGTCAACTGGCTGGCGCACAATGTGTCCAAGGATAACCGACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225827 protein sequence
Red=Cloning site Green=Tags(s) MPPGVDCPMEFWTKEESQSVVVDFLLPTGVYLNFPVSRNANLSTIKQVLWHRAQYEPLFHMLSDPEAYVF TCVNQTAEQQELEDEQRRLCDIQPFLPVLRLVAREGDRVKKLINSQISLLIGKGLHEFDSLRDPEVNDFR TKMRQFCEEAAAHRQQLGWVEWLQYSFPLQLEPSARGWRAGLLRVSNRALLVNVKFEGSEESFTFQVSTK DMPLALMACALRKKATVFRQPLVEQPEEYALQVNGRHEYLYGNYPLCHFQYICSCLHSGLTPHLTMVHSS SILAMRDEQSNPAPQVQKPRAKPPPIPAKKPSSVSLWSLEQPFSIELIEGRKVNADERMKLVVQAGLFHG NEMLCKTVSSSEVNVCSEPVWKQRLEFDISVCDLPRMARLCFALYAVVEKAKKARSTKKKSKKADCPIAW ANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPAGTVRGNPNTESAAALVIYLPEVAPHPVYFPALEK ILELGRHGERGRITEEEQLQLREILERRGSGELYEHEKDLVWKMRHEVQEHFPEALARLLLVTKWNKHED VAQMLYLLCSWPELPVLSALELLDFSFPDCYVGSFAIKSLRKLTDDELFQYLLQLVQVLKYESYLDCELT KFLLGRALANRKIGHFLFWHLRSEMHVPSVALRFGLIMEAYCRGSTHHMKVLMKQGEALSKLKALNDFVK VSSQKTTKPQTKEMMHMCMRQETYMEALSHLQSPLDPSTLLEEVCVEQCTFMDSKMKPLWIMYSSEEAGS AGNVGIIFKNGDDLRQDMLTLQMIQLMDVLWKQEGLDLRMTPYGCLPTGDRTGLIEVVLHSDTIANIQLN KSNMAATAAFNKDALLNWLKSKNPGEALDRAIEEFTLSCAGYCVATYVLGIGDRHSDNIMIRESGQLFHI DFGHFLGNFKTKFGINRERVPFILTYDFVHVIQQGKTNNSEKFERFRGYCERAYTILRRHGLLFLHLFAL MRAAGLPELSCSKDIQYLKDSLALGKTEEEALKHFRVKFNEALRESWKTKVNWLAHNVSKDNRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008840 |
| ORF Size | 3132 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008840.3, NP_032866.2 |
| RefSeq Size | 5102 bp |
| RefSeq ORF | 3135 bp |
| Locus ID | 18707 |
| MW | 119.8 kDa |
| Gene Summary | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223319 | Pik3cd (untagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 4, (10ug) |
CNY 13,400.00 |
|
| MG225827 | Pik3cd (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd) transcript variant 4, (10ug) |
CNY 11,500.00 |
|
| MR225827L3 | Lenti ORF clone of Pik3cd (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 4 |
CNY 12,350.00 |
|
| MR225827L4 | Lenti ORF clone of Pik3cd (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 4 |
CNY 12,350.00 |
