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Lig1 (NM_010715) Mouse Tagged ORF Clone
CAT＃: MR222972
- TrueORF®
Lig1 (Myc-DDK-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 2
ORF Plasmid: tGFP
"NM_010715" in other vectors (4)
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CNY 6,720.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL033288; LigI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222972 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAAGTATTATGTCATTTTTTCAACCCACAAAAGAAGGTAAAGCGAAGAAGCCAGAGAAGGAGA CACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCGCTGAAGGAGAGGAATCAAGTGGTGCC CGAGAGTGATTCTCCAGTGAAGAGGACAGGAAGGAAGGTAGCCCAGGTTCTGAGCTGTGAAGGGGAGGAC GAAGATGAAGCCCCTGGCACCCCCAAAGTCCAGAAGCCTGTGTCAGACTCTGAACAGAGCTCTCCTCCCA GCCCTGACACATGTCCTGAGAACAGTCCTGTCTTCAACTGCAGCTCCCCCATGGACATCTCCCCATCAGG ATTCCCAAAGCGTCGAACTGCGCGGAAGCAGCTCCCCAAACGGACAATTCAAGACACTCTTGAGGAGCAG AATGAGGACAAAACCAAAACAGCTAAGAAAAGGAAGAAAGAAGAAGAGACCCCAAAAGAAAGCCTCGCAG AGGCTGAAGACGTAAAACAGAAGGAAGAAAAGGAGGGGGACCAGCTCATAGTCCCCTCTGAGCCCACAAA GTCCCCTGAGTCAGTAACCCTGACAAAGACAGAGAACATTCCAGTGTGTAAGGCAGGAGTGAAACTGAAG CCTCAGGAAGAGGAACAGAGCAAACCTCCTGCTAGAGGTGCCAAGACACTGAGCAGTTTCTTCACACCCC GAAAACCAGCAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGGCACTCTAAGGAAGGAAGAGACCAAGGG AACCCTGGATCCAGCAAATTATAATCCTTCCAAGAATAACTATCATCCCATTGAAGATGCCTGCTGGAAA CATGGCCAGAAGGTCCCTTTTCTCGCCGTGGCCCGGACATTTGAGAAGATTGAGGAGGTTTCTGCTAGGC TCAAGATGGTGGAGACATTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCTCCAGACTTGCTCCC TGTTCTCTATCTCAGCCTCAACCGCCTCGGGCCACCTCAGCAGGGACTAGAGCTGGGTGTTGGTGATGGT GTCCTCCTCAAGGCCGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGA AGGGGGATGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACTCAGAGACTCATGCTGCCTCCACCTCCACT TACTATTTCTGGAGTCTTCACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAG AAGATGGACATTATCAAGGGCCTTTTTGTTGCCTGCCGCCACTCAGAAGCCAGGTACATTGCCAGGTCCC TAAGCGGGCGGCTGCGCCTTGGCCTAGCTGAGCAGTCGGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCT CACACCCCCTGGCCAAGAATTTCCCACGGCGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGA AAGATGTGGTTGGAAGAACAAGGCATGATCTTGAAGCAGACCTTCTGTGAGGTTCCTGACCTGGATCGAA TCATCCCTGTGCTGCTGGAACATGGCCTGGAACGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTCCC TCTTAAACCAATGCTGGCTCATCCCACTCGGGGTGTCAGTGAGGTCCTAAAACGCTTTGAGGAGGTCGAC TTCACCTGTGAATATAAATACGATGGGCAGCGAGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGA TCTTCAGCAGGAACCAGGAAGACAACACAGGAAAGTATCCTGACATTATCAGCCGCATCCCCAAGATTAA ACACCCCTCAGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATC CAACCATTCCAAGTACTCACCACGCGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTGCAAGTGT GTCTGTATGCTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGTTCGCCAGCCCCTGTCTCGACGCCG GCAGCTGCTCCGTGAGAACTTCGTGGAGACAGAGGGTGAGTTTGTCTTCACCACCTCTTTGGACACCAAG GACACCGAGCAGATTGCTGAGTTCTTGGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGA CCTTGGATGTTGATGCCACTTATGAGATTGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTA CCTTGACGGTGTGGGTGACACTCTGGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCT GGCCGGTATGGAGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTTCAGGCTATATGCAAGC TGGGAACTGGATTCAGCGATGAGGAGCTGGAGGAACACCACCAGAGCCTGCAGGCCCTGGTATTGCCTAC CCCACGCCCCTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCATTGGCTGGACCCAAGCATTGTGTGG GAGGTGAAGTGTGCTGATCTTTCCCTGTCCCCCATCTACCCTGCTGCACGGGGCCTGGTGGACAAAGAAA AAGGGATCTCTCTTCGTTTTCCTCGGTTCATTCGTGTCCGTAAAGACAAGCAGCCAGAGCAGGCCACTAC CAGTAACCAGGTTGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAACAAAGCTCAGACTTGGAC TCCGACGTTGAAGACTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222972 protein sequence
Red=Cloning site Green=Tags(s) MQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGED EDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQ NEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLK PQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWK HGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDG VLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAK KMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEAR KMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVD FTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQI QPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTK DTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRA GRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVW EVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLD SDVEDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010715 |
| ORF Size | 2751 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010715.3 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 2799 bp |
| Locus ID | 16881 |
| UniProt ID | P37913 |
| MW | 102.2 kDa |
| Gene Summary | DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211169 | Lig1 (tGFP-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 2 |
CNY 7,890.00 |
|
| MG222972 | Lig1 (tGFP-tagged) - Mouse ligase I DNA ATP-dependent (Lig1) transcript variant 2, (10ug) |
CNY 7,890.00 |
|
| MR222972L3 | Lenti ORF clone of Lig1 (Myc-DDK-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 2 |
CNY 9,030.00 |
|
| MR222972L4 | Lenti ORF clone of Lig1 (mGFP-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 2 |
CNY 9,030.00 |
