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Tnik (NM_026910) Mouse Tagged ORF Clone
CAT＃: MR222144
- TrueORF®
Tnik (Myc-DDK-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1
ORF Plasmid: tGFP
"NM_026910" in other vectors (4)
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CNY 13,590.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1500031A17Rik; 4831440I19Rik; AI451411; C530008O15Rik; C630040K21Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222144 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGACTCCCCAGCTCGCAGCCTGGATGAAATCGATCTCTCCGCCCTGAGGGACCCTGCAGGGA TCTTTGAGTTGGTGGAACTTGTCGGAAATGGCACGTATGGTCAAGTTTATAAGGGTCGTCATGTCAAAAC GGGCCAGCTTGCTGCCATTAAGGTTATGGATGTCACAGGGGATGAAGAGGAAGAAATCAAACAAGAAATT AACATGTTGAAGAAATATTCTCATCACAGGAACATTGCTACATACTACGGTGCTTTTATCAAAAAGAACC CTCCTGGCATGGATGACCAACTCTGGTTGGTTATGGAGTTCTGTGGTGCTGGCTCTGTCACTGACCTGAT CAAGAACACGAAAGGCAACACATTGAAAGAGGAGTGGATTGCATACATCTGCAGGGAGATCTTACGGGGC CTGAGTCACCTGCACCAGCACAAAGTGATTCATCGAGATATCAAAGGGCAGAACGTCTTGTTGACTGAAA ATGCAGAGGTTAAGCTAGTGGATTTTGGAGTGAGTGCCCAGCTTGACCGAACTGTGGGCAGGAGGAACAC GTTCATCGGGACTCCCTACTGGATGGCACCAGAAGTCATTGCCTGTGATGAGAACCCGGATGCCACATAT GATTTCAAGAGTGACTTGTGGTCTTTGGGAATCACCGCCATTGAGATGGCAGAAGGTGCCCCCCCCCTCT GTGACATGCATCCCATGAGAGCCCTCTTCCTCATCCCACGGAACCCTGCACCTCGGCTCAAGTCTAAGAA GTGGTCAAAAAAATTCCAGTCATTTATCGAGAGCTGCTTGGTAAAGAATCACAGCCAGCGGCCAGCCACG GAGCAGTTGATGAAGCACCCATTCATACGAGACCAACCTAATGAGAGGCAGGTCCGCATCCAGCTGAAGG ACCACATTGATCGAACAAAGAAGAAGCGAGGAGAAAAAGATGAGACTGAGTATGAATACAGCGGAAGTGA GGAAGAAGAGGAAGAGAATGACTCTGGGGAACCCAGCTCCATTCTGAACCTACCAGGGGAGTCAACACTG CGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCTCAGAGGCCCTGCGGCGCCAACAGCTGG AGCAGCAGCAGCGGGAGAATGAAGAACACAAGCGGCAGCTACTGGCTGAGCGCCAGAAGCGCATCGAAGA GCAGAAGGAGCAAAGGCGGAGGCTGGAGGAGCAACAAAGGCGAGAAAAAGAGCTTCGGAAACAGCAGGAG CGGGAACAGCGCCGGCACTACGAAGAACAGATGCGTCGGGAGGAGGAGAGGAGGCGTGCCGAACATGAGC AGGAATACATCAGGCGGCAGCTAGAAGAAGAGCAAAGGCAGTTAGAGATCTTACAGCAGCAGCTACTGCA TGAGCAAGCTCTACTTCTGGAATATAAGCGCAAACAATTGGAAGAACAGAGACAAGCAGAAAGACTGCAG AGGCAGCTAAAGCAAGAGCGGGACTATCTGGTTTCCCTCCAGCATCAGCGGCAGGAGCAGAGGCCCCTGG AGAAGAAGCCACTGTACCATTACAAGGAGGGCATGAGTCCTAGTGAGAAGCCGGCCTGGGCCAAGGAGGT AGAAGAACGCTCAAGACTCAACCGACAGAGTTCACCTGCCATGCCTCACAAGGTTGCCAACAGGATCTCG GACCCCAACCTGCCCCCAAGATCAGAGTCCTTCAGCATTAGTGGGGTTCAGCCTGCAAGGACACCCCCAA TGCTCAGACCTGTTGACCCCCAGATCCCGCAGCTGGTAGCTGTCAAATCCCAGGGACCTGCCTTGACGGC CTCCCAGTCAGTACATGAGCAACCCACAAAGGGCCTGTCTGGGTTCCAGGAGGCTCTGAATGTGACCTCT CACCGGGTCGAGATGCCACGCCAGAACTCGGATCCCACCTCAGAAAACCCTCCTCTCCCCACGAGAATTG AGAAGTTTGACAGAAGCTCTTGGTTACGACAGGAAGAAGACATTCCACCAAAGGTGCCTCAAAGAACAAC TTCTATATCCCCAGCACTAGCCAGAAAGAATTCCCCTGGCAATGGCAGTGCTCTGGGCCCCAGACTTGGA TCTCAGCCCATCAGAGCAAGCAACCCTGATCTGCGCAGGACAGAGCCAGTCCTGGAGAGTTCCCTGCAGC GGACAAGCAGTGGCAGTTCCTCCAGCTCCAGCACTCCCAGCTCCCAGCCCAGCTCCCAAGGAGGCTCTCA ACCTGGCTCCCAAGCAGGATCTAGTGAGCGGTCCAGAGTGCGGGCCAACAGTAAGTCCGAAGGATCACCC GTGCTCCCCCATGAGCCTTCCAAGGTGAAACCAGAAGAATCCAGAGACATCACACGGCCCAGTCGGCCAG CTAGCTATAAGAAAGCTATAGATGAGGATCTGACGGCATTAGCCAAAGAATTACGAGAACTCCGCATTGA AGAAACAAACCGCCCCCTGAAGAAAGTGACTGATTACTCTTCCTCCAGCGAGGAGTCGGAGAGCAGTGAG GAAGAAGAGGAAGATGGAGAGAGTGAGACACATGACGGGACGGTGGCTGTCAGTGACATACCCAGACTAA TACCCACCGGAGCTCCAGGGAACAATGAGCAGTACAACATGGGGATGGTCGGGACACATGGGCTGGAAAC TTCGCATGCGGACACCTTTGGCGGCAGCATTTCAAGAGAAGGAACCTTGATGATCAGAGAGACGGCTGAA GAGAAGAAGCGATCTGGCCACAGTGACAGTAATGGATTCGCCGGTCACATCAATCTCCCAGACCTTGTAC AGCAGAGCCATTCGCCAGCTGGAACTCCCACTGAGGGGCTGGGCCGCGTCTCCACTCATTCCCAGGAGAT GGACTCTGGGGCTGAATATGGTATAGGGAGCAGCACCAAAGCCTCTTTCACCCCCTTCGTGGACCCTCGA GTGTACCAGACATCGCCCACTGATGAAGATGAAGAGGATGATGAGTCTTCAGCTGCTGCCCTGTTTACTA GCGAACTTCTTAGGCAAGAACAGGCCAAACTCAATGAAGCGAGGAAGATTTCAGTGGTAAATGTGAACCC AACAAACATTCGCCCTCATAGTGACACACCGGAAATCAGAAAATACAAGAAACGCTTCAATTCAGAAATA CTTTGTGCAGCTCTATGGGGTGTGAACCTTCTGGTGGGGACTGAAAATGGCCTGATGCTTTTGGACAGAA GTGGCCAAGGCAAAGTCTACAACCTAATCAACCGGAGGCGGTTTCAGCAGATGGATGTGCTAGAAGGACT AAATGTTCTTGTCACGATATCAGGAAAGAAGAACAAGCTCCGTGTGTACTATCTCTCATGGTTAAGAAAC AGAATCCTGCACAATGACCCAGAAGTGGAAAAGAAGCAGGGCTGGATCACTGTCGGTGACTTGGAAGGCT GCATCCATTACAAAGTCGTTAAATATGAAAGAATCAAGTTCCTGGTGATTGCCTTAAAGAATGCAGTAGA GATATATGCGTGGGCCCCTAAACCTTACCATAAGTTCATGGCATTTAAGTCTTTTGCAGATCTTCAGCAT AAGCCTCTGCTCGTTGACCTCACAGTAGAAGAAGGTCAAAGGTTAAAGGTCATATTTGGCTCACACACTG GTTTCCATGTAATTGATGTTGATTCTGGAAACTCCTACGATATCTATATACCATCCCATATTCAGGGCAA TATCACTCCTCATGCTATCGTCATCTTGCCTAAAACAGATGGAATGGAGATGCTTGTCTGCTATGAGGAT GAAGGGGTGTACGTGAACACCTACGGCCGGATCACTAAGGATGTGGTGCTCCAATGGGGAGAAATGCCCA CATCTGTGGCCTACATTCATTCCAATCAGATAATGGGCTGGGGCGAGAAAGCTATTGAGATCCGGTCAGT GGAAACAGGACATTTGGATGGAGTGTTTATGCATAAACGAGCTCAAAGGTTAAAGTTTCTATGTGAAAGA AATGATAAGGTATTTTTTGCATCCGTGCGATCTGGAGGAAGTAGCCAAGTGTTTTTCATGACCCTCAACA GAAATTCCATGATGAACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222144 protein sequence
Red=Cloning site Green=Tags(s) MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRG LSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPAT EQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTL RRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQE REQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQ RQLKQERDYLVSLQHQRQEQRPLEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRIS DPNLPPRSESFSISGVQPARTPPMLRPVDPQIPQLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTS HRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLG SQPIRASNPDLRRTEPVLESSLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERSRVRANSKSEGSP VLPHEPSKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSE EEEEDGESETHDGTVAVSDIPRLIPTGAPGNNEQYNMGMVGTHGLETSHADTFGGSISREGTLMIRETAE EKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGAEYGIGSSTKASFTPFVDPR VYQTSPTDEDEEDDESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEI LCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRN RILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQH KPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYED EGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCER NDKVFFASVRSGGSSQVFFMTLNRNSMMNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026910 |
| ORF Size | 4080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026910.1, NP_081186.1 |
| RefSeq Size | 7146 bp |
| RefSeq ORF | 4083 bp |
| Locus ID | 665113 |
| MW | 154.9 kDa |
| Gene Summary | Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224337 | Tnik (untagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1, (10ug) |
CNY 17,390.00 |
|
| MG222144 | Tnik (tGFP-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik) transcript variant 1, (10ug) |
CNY 14,920.00 |
|
| MR222144L3 | Lenti ORF clone of Tnik (Myc-DDK-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1 |
CNY 15,490.00 |
|
| MR222144L4 | Lenti ORF clone of Tnik (mGFP-tagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1 |
CNY 15,490.00 |
