Ank1 (NM_031158) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ank-1; nb; pale
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221570 representing NM_031158, codon optimized. Due to the complexity of NM_031158, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGCGGCCGCGCAGGTCGGGGTCAGACCCCGCGGCCGATGCTGCTACCAGCTTTCTGCGGGCGG CACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGACATTAACACCTGTAACCA GAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCAC AAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACATCGCTGCCCTTGCTGGTC AGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAGTCTCAGAAAGGCTTTAC TCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTACTGGAGAATGGAGCCAAT CAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCAGGGTCACGAGAATGTGG TGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTGCACATCGCGGCCCGCAA CGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATGTGCTTTCCAAGACGGGA TTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTTGCTCCTCAACAGGGGAG CCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCCCGCAGGGGGAACGTGAT CATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGGATGAATTGACACCGCTC CACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCACGGGGCACCCATCCAAG CCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCACCTCGACTGTGTCCGACT TCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTCCTCTCCATGTGGCAGCC CACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCCCAACTCCAGAGCCCTGA ATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATGGAGTTGCTGCTGAAGAC AGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAGCCTCCTTCATGGGACAC CTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAATGTGAAAGTAGAAACCC CCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGC CAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCGGCCACACAGGCATGGTG AAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCACACACCCCTACACACCG CAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCATCCCAAGCCTGCATGAC CAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGTTGGCGGAGCTGCTGCTG GAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCATGTGGCCGTCCATCACA ACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGCCCTGCCTGGAATGGCTA CACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAGTACGGAGGG TCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCAAGAGGGCCACACAGAAA TGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGTGGACTCACTCCCCTCCA CCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATGGCGTCACCGTGGATGCT ACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACATCAAGCTGGTGAAGTTCT TGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCTTGCACCAGGCAGCTCA ACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAAC GGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCA CAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCTGAGACGGTGGACGAGAT CCTGGACGTGTCTGAAGATGAAGGAACTGCTCACATATCTATAATGGGTGACGAACTTGTCGGCTCGAAG GCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACC AAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACCGTTGTGATCCGATCTGA AGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCAGCCCGGCCACAGAGACC TCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAGCTTCATGGTGGATGCCC GAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCTCCCCGGACATGCGCAGC ACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCACCCCCACTGGCTGAGGAA GAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGG AAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTGAGGAGTGAAAACGGCTC TGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCCTTAATGGCATGGATGAA GAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCACCACCGACTTCCCTCTGT ACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGTTCCCTGAGAAG CAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGAAAGTGAAGCTGGCTCTG CAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGCCACATTCAGCCCCATTG TGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATCCCGCTCCCTCCCTCATG GACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTACTGTGTAGCGTCATCGGT GGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATACGCCAATGAGTGTGCCA ACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACTGCGGAGGCTGTGCACTT TGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTGTCATTTTTGCCAAGATG AACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGTGGACAAGACCCTGGAGC AGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAAGGGATGCCTCTGTTTGC AGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCTTCCATTTCCAGTCGTTT CGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGT TCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTGAATATCACCATGCCCCC CTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATT CTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAG AACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTGGAAGACATCAACCGGAT CCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCCTCTGGGTGGACCGTGAA GGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAGGAGTGAGATTGTTAACA TGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACATGGGGACCGGGAGTACTC ATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGTCCCCCGCCTCCCTGCAG TACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGTCATAGACGCCATCCCCC TGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGGTCTGCGGGCCTCACACC CTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGAGGACTCTGACGCCATACCAGAG TGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTCTCAGGACCTGGTGGAGG ACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGCCAGCAGCGAGTTCACGCCCG AATCACAGACTCACCCTCAGTGAGGCAGGTGCTGGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAA CAGGGTTCCACAGCGGTACACCCGCAAGAAGCCACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCC CACACTCATTCCAGAGAAGGATCACCACCATCCAAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCA GGTGCTGGTGTCTACCAGGGAGCATGTGCAGAGGGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAG GAACCTAGCCTGTGGGCACCTGAGAGCGCCTTCTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTC CAGGAGAGCAGGTGACGGAGGAACAATTCACAGATGAACAGGGCAACATTGTTACCAAGAAGATCATTCG CAAAGTCGTCCGGCAGGTAGACTCGTCTGGTGCCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGA GGGAGTGGACTCCAGCCGGACCTGATAGAGGGCAGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGA AAAGGGGCAAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221570 representing NM_031158 Red=Cloning site Green=Tags(s) MAERPRRSGSDPAADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLH KEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGAN QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTG FTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPL HCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAA HCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGH LPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMV KLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLL EHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGG SANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDA TTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSN GTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGTAHISIMGDELVGSK AERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATET SDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEE EGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDE ELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLAL QAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIG GTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKM NDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSF RENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSI LSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDRE GENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQ YALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPE WKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQQRVHARITDSPSVRQVLDRSQARTLDWDK QGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGK EPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELR GSGLQPDLIEGRKGAQIVKRASLKRGKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031158
ORF Size	5544 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031158.4, NP_112435.2
RefSeq Size	8179 bp
RefSeq ORF	5547 bp
Locus ID	11733
UniProt ID	Q02357
MW	202.5 kDa
Gene Summary	Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[UniProtKB/Swiss-Prot Function]

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