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Pomgnt1 (NM_029786) Mouse Tagged ORF Clone
CAT＃: MR219132
- TrueORF®
Pomgnt1 (Myc-DDK-tagged) - Mouse protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2
ORF Plasmid: tGFP
"NM_029786" in other vectors (3)
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CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610016I07Rik; 4930467B06Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219132 representing NM_029786
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACTGGAAGCCCAGTCCCCTCATCAAGCCCTTTGGGGCTAGGAAGAAACGGAGCTGGTACCTTA CCTGGAAGTATAAACTGACAAACCAGCGGGCCCTGAGGAGATTCTGTCAGACAGGGGCTGTGCTTTTCCT GCTGGTGACCGTCATTGTCAACATCAAGTTGATCCTGGACACTCGGAGAGCAATCAGCGAGGCCAATGAA GACCCAGAACCAGAGCAAGACTATGATGAGGCCCTAGGACGTCTGGAGTCCCCTCGGCGCAGAGGCAGCA GTCCCCGACGGGTGCTGGATGTGGAAGTATACTCCAGTAGAAGCAAAGTGTATGTGGCTGTGGATGGCAC CACGGTGCTCGAGGATGAAGCCCGGGAGCAGGGCCGAGGCATCCATGTCATAGTCCTCAACCAGGCCACG GGCCACGTGATGGCAAAGCGCGTGTTTGACACATACTCTCCTCATGAGGATGAGGCCATGGTGCTGTTCC TCAACATGGTGGCCCCCGGCCGCGTGCTGATCTGCACGGTGAAGGATGAGGGTTCCTTCCACCTCAAGGA CACAGCAAAGGCTCTGCTGAGGAGCCTGGGAAGTCAGGCAGGCCCTGCCCTTGGCTGGAGGGACACCTGG GCCTTCGTGGGACGAAAAGGAGAGGCTGAGTGTCACTGGGCAGATACGGAGTTGAACCGCCGCCGCAGGC GCTTCTGCAGCAAAGTGGAGGGCTACGGGAGCGTGTGCAGCTGCAAGGACCCGACGCCTATTGAGTTCAG CCCTGACCCACTTCCAGACAATAAGGTCCTCAATGTGCCTGTGGCTGTCATTGCAGGGAACCGGCCCAAT TACCTGTACAGGATGCTGCGCTCTCTGCTCTCAGCCCAAGGGGTATCTCCACAGATGATAACGGTCTTCA TTGATGGCTACTATGAGGAGCCAATGGATGTGGTGGCGCTGTTTGGTCTGAGAGGCATCCAGCATACTCC TATCAGCATCAAGAACGCCCGTGTGTCTCAGCACTACAAGGCCAGCCTCACTGCCACTTTCAACCTGTTT CCGGAGGCCAAGTTTGCTGTGGTTCTGGAAGAGGACCTGGACATTGCCGTGGATTTTTTCAGTTTCCTGA GCCAGTCCATCCACCTGCTGGAGGAAGATGACAGCCTGTACTGCATCTCTGCCTGGAATGACCAGGGGTA TGAACACACAGCTGAGGATCCAGCACTACTGTACCGTGTGGAGACCATGCCTGGGCTAGGATGGGTGCTC AGGAAATCTTTATACAAGGAGGAGCTTGAGCCCAAGTGGCCCACACCAGAAAAGCTGTGGGATTGGGACA TGTGGATGCGGATGCCGGAACAGCGCCGGGGCCGAGAGTGTATCATCCCGGATGTTTCCCGATCCTACCA CTTTGGCATCGTCGGCCTCAACATGAACGGCTACTTTCATGAGGCCTACTTCAAGAAGCACAAGTTCAAC ACAGTTCCAGGTGTCCAGCTCAGGAATGTAGACAGTCTGAAGAAAGAAGCTTATGAAGTGGAAATTCACA GACTGCTCAGTGAGGCCGAGGTTTTGGACCACAGCAAGGACCCTTGTGAAGACTCCTTCCTGCCAGACAC GGAGGGCCACACCTATGTGGCGTTTATCCGAATGGAGACAGATGATGACTTCGCCACCTGGACTCAGCTT GCCAAGTGCCTCCATATTTGGGACCTGGATGTTCGTGGCAACCACCGGGGCCTGTGGAGATTATTTCGGA AGAAGAACCACTTTTTGGTGGTGGGGGTCCCAGCCTCCCCTTACTCAGTGAAGAAGCCACCATCAGTCAC CCCTATTTTCCTGGAGCCACCCCCAAAGGAAGAAGGAGCCCCAGGGGCTGCTGAACAAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219132 representing NM_029786
Red=Cloning site Green=Tags(s) MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAVLFLLVTVIVNIKLILDTRRAISEANE DPEPEQDYDEALGRLESPRRRGSSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQAT GHVMAKRVFDTYSPHEDEAMVLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTW AFVGRKGEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPN YLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLF PEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLGWVL RKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFN TVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKDPCEDSFLPDTEGHTYVAFIRMETDDDFATWTQL AKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAAEQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029786 |
| ORF Size | 1881 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029786.2, NP_084062.2 |
| RefSeq Size | 2637 bp |
| RefSeq ORF | 1884 bp |
| Locus ID | 68273 |
| UniProt ID | Q91X88 |
| MW | 72.2 kDa |
| Gene Summary | Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219132 | Pomgnt1 (tGFP-tagged) - Mouse protein O-linked mannose beta12-N-acetylglucosaminyltransferase (Pomgnt1) transcript variant 2, (10ug) |
CNY 6,940.00 |
|
| MR219132L3 | Lenti ORF clone of Pomgnt1 (Myc-DDK-tagged) - Mouse protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2 |
CNY 8,170.00 |
|
| MR219132L4 | Lenti ORF clone of Pomgnt1 (mGFP-tagged) - Mouse protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase (Pomgnt1), transcript variant 2 |
CNY 8,170.00 |
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