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Calcoco2 (NM_029755) Mouse Tagged ORF Clone
CAT＃: MR216075
- TrueORF®
Calcoco2 (Myc-DDK-tagged) - Mouse calcium binding and coiled-coil domain 2 (Calcoco2), transcript variant 1
ORF Plasmid: tGFP
"NM_029755" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410154J16Rik; C77254; Ndp52; Ndp52l1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216075 representing NM_029755
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGTGCCCCATACCTACCTTGCTGGAACATGGCAACTTCTCTCAGGTCCTGTTTAACAATGTGG AGAAGTTCTATGCTCCTAGAGGAGATATCATGTGCTATTACACCCTCACTGAAAAGTTCATCCCTCGACG CAAGGACTGGATTGGCATCTTTAAAGTAGGGTGGAAGACCACTCAGGAGTATTATACCTTCATGTGGGCT CCCTTGCCAAAAGACCAAAACAAGGATTCAGCCACACAGCAGGAAATCCAATTCAAAGCTTATTACCTTC CCAAGGATGTGGAGCGCTACCAGTTCTGCTATGTGGATGAAGATGGTTTAGTCCGGGGAACAAGTGTCCC TTTCCAGTTTTGTCCAGACCCTGACGAGGACATAATGGTTGTTATCAATAAGGAAAAGGTAGAAGAGATG GAACAGCTCAGTGAGGAGCTTTACCAACAAAACCAGGAGCTGAAAGACAAGTACGCTGACCTCCATGAGC AGCTACAGAGGAAGCAGGTGGCACTGGAAGCAACACAGAGGGTCAATAAGACCTTAGAACACAAAGTGGA AGAGAAGGCCTCCTGGGAGAAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAG GCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCT GGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGGA AGAGAAGGCCTCCTGGGAGGAAGAGAAGGCCTCCTGGGAGAAAGAGAAGGCCTCCTGGGAGGAAGAGAAG GCCTCCTGGGAGAAAGAGAAGGCCCCCTGGGAGGTAGAGAAGGCCCCCTGGAAGGAAGTGAAGGCCTATT GGTGGAATGATCTGCACCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216075 representing NM_029755
Red=Cloning site Green=Tags(s) MDQCPIPTLLEHGNFSQVLFNNVEKFYAPRGDIMCYYTLTEKFIPRRKDWIGIFKVGWKTTQEYYTFMWA PLPKDQNKDSATQQEIQFKAYYLPKDVERYQFCYVDEDGLVRGTSVPFQFCPDPDEDIMVVINKEKVEEM EQLSEELYQQNQELKDKYADLHEQLQRKQVALEATQRVNKTLEHKVEEKASWEKEKASWEEEKASWEEEK ASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEKEKASWEEEK ASWEKEKAPWEVEKAPWKEVKAYWWNDLHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029755 |
| ORF Size | 930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029755.3, NP_084031.2 |
| RefSeq Size | 1556 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 76815 |
| MW | 57 kDa |
| Gene Summary | Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation (By similarity). Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (By similarity). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (By similarity). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (By similarity). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211780 | Calcoco2 (untagged) - Mouse calcium binding and coiled-coil domain 2 (Calcoco2), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG216075 | Calcoco2 (tGFP-tagged) - Mouse calcium binding and coiled-coil domain 2 (Calcoco2) transcript variant 1, (10ug) |
CNY 2,850.00 |
|
| MR216075L3 | Lenti ORF clone of Calcoco2 (Myc-DDK-tagged) - Mouse calcium binding and coiled-coil domain 2 (Calcoco2), transcript variant 1 |
CNY 4,750.00 |
|
| MR216075L4 | Lenti ORF clone of Calcoco2 (mGFP-tagged) - Mouse calcium binding and coiled-coil domain 2 (Calcoco2), transcript variant 1 |
CNY 4,750.00 |
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