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Bub1 (NM_009772) Mouse Tagged ORF Clone
CAT＃: MR211557
- TrueORF®
Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2
ORF Plasmid: tGFP
"NM_009772" in other vectors (4)
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CNY 10,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL022991; Bub1a; C80208; D2Xrf87 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211557 representing NM_009772
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACCTAGAAAATGTCTTTCGCATGTTTGAAGCCCATATGCAAAGCTACACGGGTAATGACCCAC TTGGAGAATGGGAAAGCTTTATAAAGTGGGTAGAAGAGAATTTTCCTGACAATAAAGAATACTTGATGAC ATTATTAGAACATTTAATGAAGGAATTTTTACATAAGAAGAACTACCACAATGATTCAAGATTCATCAAT TATTGCTTAAAATTTGCTGAGTACAACAGTGACCGTCATCAGTTTTTTGAGTTTCTGTACAACCAGGGAA TTGGAACCAAGTCATCATATATATACATGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAGAGCTGCAGCA TGCCAGTGCTATTTTTCAGACAGGAATTCACAATGAGGCTGAACCTAAAGAACTACTACAGCAACAATAC AGGCTATTCCAAGCACGCCTTACTGGAATCCATTTGCCAGCTCAAGCTACAACCTCAGAACCTTTGCATA GTGCACAGATTTTAAACCAAGTTATGATGACAAACTCAAGTCCAGAAAAAAACTCAGCCTGTGTTCCTAG GAGTCAGGGTTCAGAATGTTCTGGTGTGGCATCTTCCACTTGTGATGAAAAGTCTAATATGGAACAAAGG GTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTGTGGCACCCAAGCCTGAGGCTCAGCAAG TTATGTACTGCAAGGAAAAGCTTATTCGTGGAGATTCAGAATTTTCTTTTGAAGAACTGAGAGCCCAGAA ATATAATCAAAGGAAGAAGCATGAGCAGTGGGTTAGTGAAGACAGAAATTATATGAAAAGGAAAGAAGCA AATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTGCATCAAGTGGTGG AATTGTCACACAAGGACCTTCCTGCTTCTGAGAACAGGCCTGATGTTAGTCTAGTATGTGTTGGACAAAA TACTTGCTCCCAGCAGGAATTGAGGGGTCCAAGTCTTTCATCCATCAGTCATCAGACCTCAGAGAGTTCA GGAGAGAAACCACAGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGCACTTTGCTGTTCC CAGCTGCCAACCTGCCAGCTCTTCCTGTTCCTGTAAGTGGCCAGTCATTGACAGACTCCAGATGTGTGAA TCAAAGTGTTCATGAATTCATGCCACAGTGTGGACCAGAAACAAAAGAAGTGTGTGAAACAAATAAAGTT GCCAGCATTAATGATTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGAACACCATGCAAAG TGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGACATGTTTCAGGCTCCAAC ACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTGGACCAAAATGAAGATGCATTTGAAGCC CAGTTTCAAAAAAATGCAGTATCTTCGGGAGATTGGGGAGTTAAAAAAATTATGACTTTGTCATCTGCTT TTCCTATTTTTGAAGATGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATAAGCCCTTAGGAGC TAGGACCTTTGGAGAACGATCTCTCAGTAAATATTCCTCGAGATCAAATGAAATGCCTCACACTGATGAG TTTATGGATGATTCAACAGTATGTGGTATTCGCTGCAACAAAACTCTAGCTCCCAGTCCTAAAAGTATAG GAGACTTTACATCTGCTGCCCAACTTTCGTCTACACCATTCCACAAATTTCCAGCAGATTTAGTACAGAT TCCAGAAGATAAAGAAAATGTGGTAGCCACACAGTATACACATATGGCTTTGGATTCTTGTAAAGAAAAC ATAGTGGACCTCTCAAAAGGCAGAAAGCTTGGGCCAATTCAAGAGAAAATTTCAGCATCTTTACCCTGTC CTAGTCAGCCTGCCACAGGTGGTTTGTTCACCCAGGAAGCAGTGTTCGGCCTTGAGGCTTTTAAATGCAC AGGCATTGACCATGCGACAGTGGAAGACCTATCCGATGCCAATGCTGGGCTCCAAGTTGAATGCGTGCAG ACACTTGGAAATGTCAATGCTCCAAGCTTTACTGTTGAGAACCCATGGGATGACGAATTGATTCTTAAAC TTCTCTCTGGACTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGAGTAAACTTCCAGC CATCAAGACCAAGACAGAATATCAATTGGGTTCTTTGCTGGTCTATGTGAATCACCTTCTTGGAGAAGGA GCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATGTGAGAAATGCCAAAAGTGAACAGAAATGCATTT TGAAGGTGCAGAGACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGGAAAGACTAAAGCC AGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCATTTATTCAAGAATGGCAGCATATTAGTAGGG GAACTCTACAGCTATGGGACGTTACTAAATGTCATTAACCTCTATAAAAATACCTCTGAAAAAGTGATGC CCCAGGCTCTTGTCCTCACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCCACAGCTGCGAAAT CATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACAGGCTGATGAAGAC TTAGCTACCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTCCCTAAAGGAACTG TATTTACAGGAAAATGTGAAACATCTGGTTTTCAGTGTCCTGAGATGCTCAGTAACAAGCCATGGAACTA CCAGATTGATTACTTTGGAGTTGCTGCAACAATATACTGTATGCTCTTTGGCTCTTACATGAAAGTAAAA AATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATATGTGGGAGGAAT TTTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGATTTTCTGAGACAGAATAT GAAGAAATTACTTGAACAACAGTATTCCAACAAGATTAAGACCTTGCGTAATAGGCTAATTGTGATGCTT TCAGAATATAAGCGTTCAAGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211557 representing NM_009772
Red=Cloning site Green=Tags(s) MDNLENVFRMFEAHMQSYTGNDPLGEWESFIKWVEENFPDNKEYLMTLLEHLMKEFLHKKNYHNDSRFIN YCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQQY RLFQARLTGIHLPAQATTSEPLHSAQILNQVMMTNSSPEKNSACVPRSQGSECSGVASSTCDEKSNMEQR VIMISKSECSVSSSVAPKPEAQQVMYCKEKLIRGDSEFSFEELRAQKYNQRKKHEQWVSEDRNYMKRKEA NAFEEQLLKQKMDELHKKLHQVVELSHKDLPASENRPDVSLVCVGQNTCSQQELRGPSLSSISHQTSESS GEKPQEEPSVPLMVNAVNSTLLFPAANLPALPVPVSGQSLTDSRCVNQSVHEFMPQCGPETKEVCETNKV ASINDFHTTPNTSLGMVQGTPCKVQPSPTVHTKEALGFIMDMFQAPTLPDISDDKDEWPSLDQNEDAFEA QFQKNAVSSGDWGVKKIMTLSSAFPIFEDGNKENYGLPQPKNKPLGARTFGERSLSKYSSRSNEMPHTDE FMDDSTVCGIRCNKTLAPSPKSIGDFTSAAQLSSTPFHKFPADLVQIPEDKENVVATQYTHMALDSCKEN IVDLSKGRKLGPIQEKISASLPCPSQPATGGLFTQEAVFGLEAFKCTGIDHATVEDLSDANAGLQVECVQ TLGNVNAPSFTVENPWDDELILKLLSGLSKPVTSYSNTFEWQSKLPAIKTKTEYQLGSLLVYVNHLLGEG AFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFIKFYSAHLFKNGSILVG ELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQADED LATGLALIDLGQSIDMKLFPKGTVFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVK NEGGVWKPEGLFRRLPHLDMWEEFFHIMLNIPDCHNLPSLDFLRQNMKKLLEQQYSNKIKTLRNRLIVML SEYKRSRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009772 |
| ORF Size | 3174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009772.2, NP_033902.2 |
| RefSeq Size | 4334 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 12235 |
| UniProt ID | O08901 |
| MW | 120 kDa |
| Gene Summary | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. Essential during early and later stages of embryonic development. Necessary for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts and plays an important role in spermatogenesis and fertility.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204570 | Bub1 (untagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2, (10ug) |
CNY 7,384.00 |
|
| MG211557 | Bub1 (tGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S |
CNY 11,590.00 |
|
| MR211557L3 | Lenti ORF clone of Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2 |
CNY 12,450.00 |
|
| MR211557L4 | Lenti ORF clone of Bub1 (mGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 2 |
CNY 12,450.00 |
