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Bpgm (NM_007563) Mouse Tagged ORF Clone
CAT＃: MR203358
- TrueORF®
Bpgm (Myc-DDK-tagged) - Mouse 2,3-bisphosphoglycerate mutase (Bpgm)
ORF Plasmid: tGFP
"NM_007563" in other vectors (3)
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CNY 2,081.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI323730; AL022789; C86192 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203358 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAGCACAAACTTATTATACTAAGACATGGAGAGGGCCAATGGAATAAGGAGAACCGATTCTGTA GTTGGGTGGACCAGAAACTTAACAACGACGGACTGGAGGAGGCTCGGAACTGTGGGAGGCAGCTCAAAGC TCTCAACTTCGAGTTTGATCTTGTCTTCACATCCATCCTTAACAGGTCCATTCACACAGCCTGGCTGATC CTGGAAGAGCTGGGGCAGGAGTGGGTGCCTGTGGAGAGCTCCTGGCGTCTGAATGAGCGTCACTATGGAG CCTTGATTGGCCTCAACAGGGAGAAAATGGCTTTGAATCATGGTGAAGAGCAGGTAAGGCTCTGGAGGAG AAGCTACAACGTGACCCCACCTCCTATAGAGGAGTCTCATCCCTACTTCCACGAGATCTACAGTGACCGG AGGTACAAAGTGTGCGATGTGCCCTTGGACCAACTGCCACGTTCAGAAAGCTTGAAGGATGTTCTGGAGA GACTTCTTCCCTACTGGAAGGAAAGGATTGCTCCGGAAATCTTAAAGGGCAAAAGCATTCTGATATCTGC TCACGGGAATAGCAGTAGAGCTCTCCTGAAACATCTGGAAGGTATCTCAGACGAGGATATTATCAACATC ACTCTGCCCACTGGAGTTCCGATTCTGCTGGAGTTGGATGAGAACCTGCGTGCCGTTGGGCCTCACCAGT TCCTGGGCAACCAGGAGGCCATCCAGGCAGCCATTAAGAAAGTGGATGATCAAGGGAAAGTGAAACAAGG CAAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203358 protein sequence
Red=Cloning site Green=Tags(s) MSKHKLIILRHGEGQWNKENRFCSWVDQKLNNDGLEEARNCGRQLKALNFEFDLVFTSILNRSIHTAWLI LEELGQEWVPVESSWRLNERHYGALIGLNREKMALNHGEEQVRLWRRSYNVTPPPIEESHPYFHEIYSDR RYKVCDVPLDQLPRSESLKDVLERLLPYWKERIAPEILKGKSILISAHGNSSRALLKHLEGISDEDIINI TLPTGVPILLELDENLRAVGPHQFLGNQEAIQAAIKKVDDQGKVKQGKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007563 |
| ORF Size | 780 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007563.1, NM_007563.2, NM_007563.3, NM_007563.4, NP_031589.1 |
| RefSeq Size | 2068 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 12183 |
| UniProt ID | P15327 |
| MW | 30 kDa |
| Gene Summary | Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.11) activity.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bisphosphoglycerate Mutase Deficiency Protects against Cerebral Malaria and Severe Malaria-Induced Anemia
,Xu, G;van Bruggen, R;Gualtieri, CO;Moradin, N;Fois, A;Vallerand, D;De Sa Tavares Russo, M;Bassenden, A;Lu, W;Tam, M;Lesage, S;Girouard, H;Avizonis, DZ;Deblois, G;Prchal, JT;Stevenson, M;Berghuis, A;Muir, T;Rabinowitz, J;Vidal, SM;Fodil, N;Gros, P;,
Cell Rep
,PubMed ID 32966787
[BPGM]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203358 | Bpgm (tGFP-tagged) - Mouse 2,3-bisphosphoglycerate mutase (Bpgm) |
CNY 2,850.00 |
|
| MR203358L3 | Lenti ORF clone of Bpgm (Myc-DDK-tagged) - Mouse 2,3-bisphosphoglycerate mutase (Bpgm) |
CNY 4,750.00 |
|
| MR203358L4 | Lenti ORF clone of Bpgm (mGFP-tagged) - Mouse 2,3-bisphosphoglycerate mutase (Bpgm) |
CNY 4,750.00 |
