United States
Germany
Japan
United Kingdom
China
Grin2b (NM_008171) Mouse Tagged ORF Clone
CAT＃: MG227077
- TrueORF®
Grin2b (tGFP-tagged) - Mouse glutamate receptor ionotropic NMDA2B (epsilon 2) (Grin2b), (10ug)
ORF Plasmid: DDK
"NM_008171" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW490526; GluN2B; Nmdar2b; NR2B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227077 representing NM_008171
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCCAGCGCAGAGTGCTGTTCTCCCAAGTTCTGGTTGGTGTTGGCCGTCTTGGCCGTATCGGGCA GCAAAGCTCGTTCCCAAAAGAGCGCCCCCAGCATCGGCATCGCTGTCATCCTCGTGGGCACTTCCGACGA AGTGGCCATAAAAGATGCCCACGAGAAAGATGACTTCCATCATCTCTCAGTAGTTCCCCGGGTGGAGCTG GTAGCCATGAACGAGACTGACCCAAAGAGCATAATCACCCGCATCTGCGATCTTATGTCTGACCGGAAGA TCCAGGGGGTGGTGTTCGCGGATGACACGGACCAGGAAGCCATCGCCCAGATCCTCGATTTCATTTCTGC TCAGACTCTCACCCCCATCCTGGGCATCCATGGGGGCTCATCTATGATAATGGCAGATAAGGATGAGTCC TCCATGTTCTTCCAGTTTGGCCCATCCATTGAACAGCAAGCTTCTGTCATGCTCAACATCATGGAAGAAT ACGACTGGTACATCTTCTCCATCGTCACCACCTACTTCCCCGGCTACCAGGACTTCGTGAACAAGATCCG CAGCACTATTGAGAACAGCTTTGTGGGCTGGGAGCTCGAGGAAGTCCTCCTGCTAGACATGTCTCTAGAC GATGGCGACTCTAAGATCCAGAATCAGCTGAAGAAGCTGCAGAGCCCCATCATTCTCCTCTACTGCACAA AGGAAGAAGCCACCTACATCTTCGAAGTAGCTAACTCAGTTGGGCTGACTGGCTACGGCTACACATGGAT CGTGCCGAGTCTGGTGGCGGGGGATACGGACACGGTGCCTTCAGAGTTCCCCACGGGGCTCATCTCTGTG TCATATGACGAATGGGACTATGGCCTTCCTGCCAGAGTGAGAGATGGGATTGCCATCATCACCACTGCTG CCTCGGACATGCTGTCCGAACACAGTTTCATCCCTGAGCCCAAGAGCAGTTGCTACAACACCCACGAGAA GAGGATCTACCAGTCTAACATGCTGAATAGGTATCTGATCAACGTCACTTTTGAAGGGAGAAACCTGTCC TTCAGTGAAGATGGCTACCAGATGCATCCGAAGCTGGTGATAATCCTTCTGAACAAGGAGAGGAAGTGGG AGAGGGTGGGAAAATGGAAAGACAAGTCCCTGCAGATGAAATACTATGTGTGGCCTCGAATGTGTCCTGA GACTGAAGAACAGGAAGATGACCATCTGAGCATCGTTACCTTGGAGGAGGCACCGTTTGTCATTGTGGAA AGTGTGGACCCTCTCAGTGGGACCTGCATGCGGAATACAGTCCCGTGCCAGAAGCGCATCATCTCTGAGA ATAAAACAGATGAGGAACCAGGCTACATCAAAAAATGCTGCAAGGGGTTTTGTATTGATATCCTTAAGAA AATTTCTAAGTCTGTGAAGTTCACCTATGACCTTTACCTGGTGACCAATGGCAAGCATGGAAAGAAAATC AACGGGACCTGGAACGGCATGATTGGTGAGGTGGTCATGAAGAGGGCCTATATGGCAGTGGGATCACTAA CTATCAATGAAGAACGGTCAGAGGTGGTTGACTTCTCTGTGCCCTTCATAGAGACTGGCATCAGTGTCAT GGTATCACGCAGCAATGGGACTGTGTCACCTTCTGCCTTCTTAGAGCCATTCAGTGCTGACGTGTGGGTG ATGATGTTTGTGATGCTGCTCATTGTCTCTGCTGTAGCTGTCTTTGTCTTTGAATACTTCAGCCCTGTGG GTTACAACCGGTGCCTAGCTGATGGCAGAGAGCCAGGCGGCCCATCTTTCACCATCGGCAAAGCGATTTG GTTACTCTGGGGTCTGGTGTTTAACAACTCCGTACCTGTGCAGAACCCAAAGGGGACCACCTCCAAGATC ATGGTGTCAGTGTGGGCCTTCTTTGCTGTCATTTTCCTGGCCAGCTACACTGCCAACTTAGCCGCCTTCA TGATCCAAGAGGAGTATGTGGACCAGGTTTCCGGCCTGAGTGACAAGAAGTTCCAGAGACCTAATGACTT CTCACCCCCTTTCCGCTTTGGGACTGTGCCCAATGGCAGCACAGAGAGGAATATCCGTAATAACTATGCA GAAATGCATGCCTACATGGGAAAGTTCAACCAAAGGGGTGTAGATGATGCCTTGCTCTCCCTGAAAACAG GGAAACTTGATGCATTCATCTACGATGCAGCCGTGCTCAACTACATGGCTGGAAGAGACGAAGGCTGCAA GCTGGTGACCATTGGCAGTGGCAAGGTCTTTGCTTCTACGGGCTATGGCATTGCTATCCAAAAAGACTCT GGTTGGAAACGCCAGGTGGACCTTGCTATCCTGCAGCTGTTTGGAGATGGGGAGATGGAAGAACTGGAAG CTCTCTGGCTCACTGGCATTTGCCACAATGAGAAGAATGAGGTTATGAGCAGCCAGCTGGACATTGACAA CATGGCGGGCGTCTTCTATATGTTGGGGGCAGCCATGGCTCTCAGCCTCATCACCTTCATCTGTGAACAT CTCTTCTATTGGCAGTTCCGACATTGCTTCATGGGTGTCTGTTCTGGCAAGCCTGGCATGGTCTTCTCCA TCAGCAGAGGTATCTACAGCTGTATCCACGGAGTAGCTATAGAGGAGCGCCAATCCGTGATGAACTCCCC CACTGCCACCATGAACAACACACACTCCAATATCCTACGCTTGCTCCGAACGGCCAAAAACATGGCCAAC CTGTCTGGAGTCAACGGCTCCCCCCAGAGTGCCCTGGACTTCATCCGCCGTGAGTCCTCTGTCTATGACA TCTCTGAGCATCGCCGCAGCTTCACGCATTCAGACTGCAAGTCGTACAATAACCCACCCTGTGAGGAAAA CCTGTTCAGTGACTACATTAGTGAGGTAGAGAGAACATTTGGCAACCTGCAGCTGAAGGACAGCAATGTG TACCAAGACCACTATCACCATCACCACCGGCCCCACAGCATCGGCAGCACCAGCTCCATTGATGGGCTCT ATGACTGTGACAACCCACCCTTTACCACCCAGCCCAGGTCAATCAGCAAGAAACCCCTGGACATTGGCCT GCCCTCCTCCAAACACAGCCAGCTCAGCGACCTGTACGGCAAGTTCTCTTTCAAGAGTGACCGCTACAGT GGCCATGATGACTTGATTCGATCAGATGTCTCAGACATCTCCACGCATACTGTCACCTATGGCAACATCG AGGGCAACGCAGCCAAGAGGAGGAAGCAGCAATATAAGGACAGTCTAAAGAAGCGGCCAGCCTCGGCCAA ATCTAGGAGGGAGTTTGATGAAATCGAGCTGGCCTACCGTCGCCGACCACCCCGCTCCCCAGACCACAAG CGCTACTTCAGGGACAAAGAAGGGCTCCGAGACTTCTACCTGGACCAGTTCCGAACAAAGGAGAACTCGC CTCACTGGGAGCACGTGGACTTGACTGACATTTACAAAGAACGTAGTGATGACTTCAAGCGAGATTCGGT CAGTGGAGGCGGGCCCTGTACCAACAGGTCTCACCTTAAACACGGAACAGGCGATAAGCACGGAGTGGTA GGCGGGGTGCCTGCTCCTTGGGAGAAGAACCTGACCAATGTGGATTGGGAGGATAGGTCTGGGGGCAACT TCTGCCGCAGCTGTCCCTCCAAGCTGCACAATTACTCCTCTACGGTGGCAGGGCAAAACTCGGGCCGGCA GGCCTGCATCAGGTGTGAGGCCTGCAAGAAGGCTGGCAACCTGTATGACATCAGCGAGGACAACTCCCTG CAGGAACTGGACCAGCCTGCTGCCCCTGTGGCTGTGTCATCCAACGCCTCCACCACCAAGTATCCTCAAA GCCCGACTAATTCCAAGGCCCAGAAGAAGAATCGGAACAAACTGCGCCGGCAGCACTCCTACGACACCTT CGTGGACCTGCAGAAGGAGGAGGCCGCCTTGGCCCCACGCAGCGTGAGCCTGAAAGACAAGGGCCGATTC ATGGATGGGAGCCCCTACGCCCATATGTTTGAGATGCCAGCTGGTGAGAGCTCCTTTGCCAACAAGTCCT CAGTGACCACTGCCGGACACCATCACAACAATCCCGGCAGCGGCTACATGCTCAGCAAGTCGCTCTACCC TGACCGGGTCACGCAAAACCCTTTCATCCCCACTTTTGGGGATGATCAGTGCTTGCTTCACGGCAGCAAA TCCTACTTCTTCAGGCAGCCCACGGTGGCAGGGGCGTCGAAAACAAGGCCGGACTTCCGGGCCCTTGTCA CCAATAAGCCAGTGGTGTCAGCCCTTCATGGGGCTGTGCCAGGTCGTTTCCAGAAGGACATTTGTATAGG GAACCAGTCCAACCCCTGTGTGCCTAACAACAAAAACCCCAGGGCTTTCAATGGCTCCAGCAATGGACAT GTTTATGAGAAACTTTCTAGTATTGAGTCTGATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227077 representing NM_008171
Red=Cloning site Green=Tags(s) MKPSAECCSPKFWLVLAVLAVSGSKARSQKSAPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVEL VAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDES SMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLD DGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLISV SYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLS FSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVE SVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKI NGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWV MMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKI MVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYA EMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDS GWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEH LFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMAN LSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNV YQDHYHHHHRPHSIGSTSSIDGLYDCDNPPFTTQPRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYS GHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHK RYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHLKHGTGDKHGVV GGVPAPWEKNLTNVDWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDISEDNSL QELDQPAAPVAVSSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRF MDGSPYAHMFEMPAGESSFANKSSVTTAGHHHNNPGSGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSK SYFFRQPTVAGASKTRPDFRALVTNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGH VYEKLSSIESDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008171 |
| ORF Size | 4446 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008171.4 |
| RefSeq Size | 7515 bp |
| RefSeq ORF | 4449 bp |
| Locus ID | 14812 |
| UniProt ID | Q01097 |
| Gene Summary | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1377365, PubMed:26912815). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:1377365). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (PubMed:20141836). Contributes to neural pattern formation in the developing brain (PubMed:8789948). Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (PubMed:8789948).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224542 | Grin2b (untagged) - Mouse glutamate receptor, ionotropic, NMDA2B (epsilon 2) (Grin2b), (10ug) |
CNY 10,144.00 |
|
| MR227077 | Grin2b (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2B (epsilon 2) (Grin2b) |
CNY 10,144.00 |
|
| MR227077L3 | Lenti ORF clone of Grin2b (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2B (epsilon 2) (Grin2b) |
CNY 12,730.00 |
|
| MR227077L4 | Lenti ORF clone of Grin2b (mGFP-tagged) - Mouse glutamate receptor, ionotropic, NMDA2B (epsilon 2) (Grin2b) |
CNY 12,730.00 |
