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Bub1 (NM_001113179) Mouse Tagged ORF Clone
CAT＃: MG226889
- TrueORF®
Bub1 (tGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001113179" in other vectors (3)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AL022991; Bub1a; C80208; D2Xrf87 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226889 representing NM_001113179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACCTAGAAAATGTCTTTCGCATGTTTGAAGCCCATATGCAAAGCTACACGGGTAATGACCCAC TTGGAGAATGGGAAAGCTTTATAAAGTGGGTAGAAGAGAATTTTCCTGACAATAAAGAATACTTGATGAC ATTATTAGAACATTTAATGAAGGAATTTTTACATAAGAAGAACTACCACAATGATTCAAGATTCATCAAT TATTGCTTAAAATTTGCTGAGTACAACAGCGACCGTCATCAGTTTTTTGAGTTTCTGTACAACCAGGGAA TTGGAACCAAGTCATCATATATATACATGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAGAGCTGCAGCA TGCCAGTGCTATTTTTCAGACAGGAATTCACAATGAGGCTGAACCTAAAGAACTACTACAGCAACAATAC AGGCTATTCCAAGCACGCCTTACTGGAATCCATTTGCCAGCTCAAGCTACAACCTCAGAACCTTTGCATA GTGCACAGATTTTAAACCAAGTTATGATGACAAACTCAAGTCCAGAAAAAAACTCAGCCTGTGTTCCTAA GAGTCAGGGTTCAGAATGTTCTGGTGTGGCATCTTCCACTTGTGATGAAAAGTCTAATATTAGGGAACAA AGGGTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTGTGGCACCCAAGCCTGAGGCTCAGC AAGTTATGTACTGCAAGGAAAAGCTTATTCGTGGAGATTCAGAATTTTCTTTTGAAGAACTGAGAGCCCA GAAATATAATCAAAGGAAGAAGCATGAGCAGTGGGTTAGTGAAGACAGAAATTATATGAAAAGGAAAGAA GCAAATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTGCATCAAGTGG TGGAATTGTCACACAAGGACCTTCCTGCTTCTGAGAACAGGCCTGATGTTAGTCTAGTATGTGTTGGACA AAATACTTGCTCCCAGCAGGAATTGAGGGGTCCAAGTCTTTCATCCATCAGTCATCAGACCTCAGAGAGT TCAGGAGAGAAACCACAGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGCACTTTGCTGT TCCCAGCTGCCAACCTGCCAGCTCTTCCTGTTCCTGTAAGTGGCCAGTCATTGACAGACTCCAGATGTGT GAATCAAAGTGTTCATGAATTCATGCCACAGTGTGGACCAGAAACAAAAGAAGTGTGTGAAACAAATAAA GTTGCCAGCATTAATGATTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGAACACCATGCA AAGTGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGACATGTTTCAGGCTCC AACACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTGGACCAAAATGAAGATGCATTTGAA GCCCAGTTTCAAAAAAATGCAGTATCTTCGGGAGATTGGGGAGTTAAAAAAATTATGACTTTGTCATCTG CTTTTCCTATTTTTGAAGATGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATAAGCCCTTAGG AGCTAGGACCTTTGGAGAACGATCTCTCAGTAAATATTCCTCGAGATCAAATGAAATGCCTCACACTGAT GAGTTTATGGATGATTCAACAGTATGTGGTATTCGCTGCAACAAAACTCTAGCTCCCAGTCCTAAAAGTA TAGGAGACTTTACATCTGCTGCCCAACTTTCGTCTACACCATTCCACAAATTTCCAGCAGATTTAGTACA GATTCCAGAAGATAAAGAAAATGTGGTAGCCACACAGTATACACATATGGCTTTGGATTCTTGTAAAGAA AACATAGTGGACCTCTCAAAAGGCAGAAAGCTTGGGCCAATTCAAGAGAAAATTTCAGCATCTTTACCCT GTCCTAGTCAGCCTGCCACAGGTGGTTTGTTCACCCAGGAAGCAGTGTTCGGCCTTGAGGCTTTTAAATG CACAGGCATTGACCATGCGACAGTGGAAGACCTATCCGATGCCAATGCTGGGCTCCAAGTTGAATGCGTG CAGACACTTGGAAATGTCAATGCTCCAAGCTTTACTGTTGAGAACCCATGGGATGATGAATTGATTCTTA AACTTCTCTCTGGACTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGAGTAAACTTCC AGCCATCAAGACCAAGACAGAATATCAATTGGGTTCTTTGCTGGTCTATGTGAATCACCTTCTTGGAGAA GGAGCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATGTGAGAAATGCCAAAAGTGAACAGAAATGCA TTTTGAAGGTGCAGAGACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGGAAAGACTAAA GCCAGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCATTTATTCAAGAACGGCAGCATATTAGTA GGGGAACTCTACAGCTATGGGACGTTACTAAATGTCATTAACCTCTATAAAAATACCTCTGAAAAAGTGA TGCCCCAGGCTCTTGTCCTCACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCCACAGCTGCGA AATCATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACAGGCTGATGAA GACTTAGCTACCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTCCCTAAAGGAA CTGTATTTACAGGAAAATGTGAAACATCTGGTTTTCAGTGTCCTGAGATGCTCAGTAACAAGCCATGGAA CTACCAGATTGATTACTTTGGAGTTGCTGCAACAATATACTGTATGCTCTTTGGCTCTTACATGAAAGTA AAAAATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATATGTGGGAGG AATTTTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGATTTTCTGAGACAGAA TATGAAGAAATTACTTGAACAACAGTATTCCAACAAGATTAAGACCTTGCGTAATAGGCTAATTGTGATG CTTTCAGAATATAAGCGTTCAAGAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226889 representing NM_001113179
Red=Cloning site Green=Tags(s) MDNLENVFRMFEAHMQSYTGNDPLGEWESFIKWVEENFPDNKEYLMTLLEHLMKEFLHKKNYHNDSRFIN YCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQQY RLFQARLTGIHLPAQATTSEPLHSAQILNQVMMTNSSPEKNSACVPKSQGSECSGVASSTCDEKSNIREQ RVIMISKSECSVSSSVAPKPEAQQVMYCKEKLIRGDSEFSFEELRAQKYNQRKKHEQWVSEDRNYMKRKE ANAFEEQLLKQKMDELHKKLHQVVELSHKDLPASENRPDVSLVCVGQNTCSQQELRGPSLSSISHQTSES SGEKPQEEPSVPLMVNAVNSTLLFPAANLPALPVPVSGQSLTDSRCVNQSVHEFMPQCGPETKEVCETNK VASINDFHTTPNTSLGMVQGTPCKVQPSPTVHTKEALGFIMDMFQAPTLPDISDDKDEWPSLDQNEDAFE AQFQKNAVSSGDWGVKKIMTLSSAFPIFEDGNKENYGLPQPKNKPLGARTFGERSLSKYSSRSNEMPHTD EFMDDSTVCGIRCNKTLAPSPKSIGDFTSAAQLSSTPFHKFPADLVQIPEDKENVVATQYTHMALDSCKE NIVDLSKGRKLGPIQEKISASLPCPSQPATGGLFTQEAVFGLEAFKCTGIDHATVEDLSDANAGLQVECV QTLGNVNAPSFTVENPWDDELILKLLSGLSKPVTSYSNTFEWQSKLPAIKTKTEYQLGSLLVYVNHLLGE GAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFIKFYSAHLFKNGSILV GELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQADE DLATGLALIDLGQSIDMKLFPKGTVFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKV KNEGGVWKPEGLFRRLPHLDMWEEFFHIMLNIPDCHNLPSLDFLRQNMKKLLEQQYSNKIKTLRNRLIVM LSEYKRSRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113179 |
| ORF Size | 3177 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113179.1, NP_001106650.1 |
| RefSeq Size | 4337 bp |
| RefSeq ORF | 3180 bp |
| Locus ID | 12235 |
| UniProt ID | O08901 |
| Gene Summary | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. Essential during early and later stages of embryonic development. Necessary for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts and plays an important role in spermatogenesis and fertility.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR226889 | Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 1 |
CNY 7,376.00 |
|
| MR226889L3 | Lenti ORF clone of Bub1 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 1 |
CNY 10,260.00 |
|
| MR226889L4 | Lenti ORF clone of Bub1 (mGFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), transcript variant 1 |
CNY 10,260.00 |
