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Sema4c (NM_001126047) Mouse Tagged ORF Clone
CAT＃: MG226572
- TrueORF®
Sema4c (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4C (Sema4c), (10ug)
ORF Plasmid: DDK
"NM_001126047" in other vectors (4)
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CNY 10,760.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI426163; M-Sema F; Se; Sem; Sema; Semacl1; Semaf; Semai; sema I |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226572 representing NM_001126047
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCACACTGGGCTGTCTGGCTGCTGGCAGCAGGGCTGTGGGGCCTGGGCATCGGGGCTGAGATGT GGTGGAACCTTGTGCCCCGGAAGACAGTATCTTCTGGGGAGCTGGTCACAGTAGTGAGGCGGTTCTCCCA GACAGGCATCCAGGACTTCCTGACACTGACCCTGACAGAACATTCTGGCCTTTTATATGTGGGGGCCCGA GAGGCGCTGTTTGCCTTCAGTGTAGAGGCTCTGGAGCTGCAAGGAGCGATCTCTTGGGAGGCTCCAGCTG AGAAGAAAATTGAATGTACCCAGAAAGGGAAGAGCAACCAGACCGAATGCTTCAACTTCATCCGCTTCCT TCAGCCATACAATTCCTCCCATCTGTATGTCTGCGGCACCTATGCCTTCCAGCCCAAGTGCACCTACATC AACATGCTCACGTTCACCTTGGACCGTGCAGAATTTGAGGATGGGAAGGGTAAATGCCCATATGACCCAG CTAAGGGTCACACCGGACTCCTTGTGGACGGTGAGCTGTACTCAGCCACACTCAATAACTTCCTGGGCAC AGAGCCGGTTATCCTTCGATACATGGGGACCCACCACTCCATCAAGACAGAGTACCTGGCTTTTTGGCTG AATGAACCCCACTTTGTAGGCTCTGCCTTTGTCCCTGAGAGTGTGGGAAGCTTCACGGGAGACGATGACA AGATCTACTTCTTCTTCAGTGAGCGGGCAGTGGAGTATGACTGCTATTCCGAGCAGGTGGTGGCTCGTGT GGCGAGAGTCTGTAAGGGTGACATGGGGGGAGCACGGACGCTGCAGAAGAAATGGACGACGTTCCTGAAG GCTCGGTTGGTGTGCTCAGCCCCTGACTGGAAGGTCTACTTCAACCAGCTGAAGGCGGTGCACACCCTGC GGGGCGCCTCTTGGCACAACACCACCTTCTTCGGGGTTTTTCAAGCGCGATGGGGCGATATGGACCTGTC TGCAGTTTGTGAGTACCAGTTGGAACAGATCCAGCAAGTGTTTGAGGGTCCCTACAAGGAGTACAGTGAG CAAGCCCAGAAGTGGGCCCGCTATACTGACCCGGTACCCAGCCCTCGGCCTGGTTCGTGTATCAACAACT GGCACCGAGACAATGGCTACACCAGTTCCCTGGAACTGCCGGACAACACCCTCAACTTCATCAAGAAGCA CCCCCTGATGGAGGACCAGGTGAAGCCTCGGTTGGGCCGCCCCCTACTTGTGAAGAAGAACACTAACTTC ACACACGTGGTGGCCGACAGGGTCCCAGGGCTTGATGGTGCCACCTATACAGTGTTGTTCATTGGTACAG GAGATGGCTGGCTGCTGAAGGCTGTGAGCCTGGGGCCCTGGATCCACATGGTGGAGGAACTGCAGGTGTT TGACCAGGAGCCAGTGGAAAGTCTGGTGCTGTCTCAGAGCAAGAAGGTGCTCTTTGCTGGCTCCCGCTCT CAGCTGGTTCAGCTGTCTCTGGCCGACTGCACAAAGTACCGTTTCTGTGTAGACTGTGTCCTGGCCAGGG ACCCTTACTGTGCCTGGAATGTCAACACCAGCCGCTGTGTGGCCACCACCAGTGGTCGCTCGGGGTCCTT TCTGGTCCAACATGTGGCGAACTTGGACACTTCAAAGATGTGTAACCAGTATGGCATTAAAAAAGTCAGA TCTATTCCCAAGAACATCACCGTTGTGTCAGGCACAGACCTGGTCCTACCCTGCCACCTCTCGTCCAATT TGGCCCATGCCCACTGGACCTTCGGAAGCCAGGACCTGCCTGCAGAACAACCTGGCTCCTTTCTTTATGA CACGGGACTCCAGGCGCTGGTGGTGATGGCCGCACAGTCCCGTCACTCTGGACCCTATCGTTGCTATTCA GAGGAGCAGGGGACAAGACTGGCTGCAGAAAGCTACCTTGTTGCTGTCGTGGCCGGCTCGTCGGTGACAC TGGAGGCACGGGCTCCCTTGGAAAACCTGGGGCTCGTGTGGCTCGCTGTGGTGGCCCTGGGGGCTGTGTG CCTGGTGCTGCTGCTGCTGGTCCTATCGCTCCGCCGGCGACTTCGAGAAGAGCTAGAAAAGGGTGCCAAG GCATCTGAGAGGACACTGGTGTACCCCTTGGAACTGCCCAAGGAGCCTGCCAGTCCCCCCTTCCGTCCTG GCCCCGAAACTGATGAGAAACTTTGGGATCCTGTCGGGTACTACTATTCGGATGGCTCTCTCAAGATTGT GCCTGGTCACGCCCGGTGCCAGCCTGGGGGTGGGCCCCCTTCCCCACCTCCTGGCATACCTGGCCAGCCT CTGCCTTCTCCAACTCGGCTCCACCTAGGAGGTGGTCGGAACTCAAATGCCAATGGTTATGTGCGTTTAC AGTTGGGCGGAGAGGACCGAGGAGGATCTGGGCACCCACTGCCTGAGCTCGCGGATGAATTACGACGGAA ACTACAACAGCGCCAGCCGCTGCCTGACTCCAACCCAGAGGAGTCTTCAGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226572 representing NM_001126047
Red=Cloning site Green=Tags(s) MAPHWAVWLLAAGLWGLGIGAEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAR EALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYI NMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWL NEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLK ARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSE QAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNF THVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRS QLVQLSLADCTKYRFCVDCVLARDPYCAWNVNTSRCVATTSGRSGSFLVQHVANLDTSKMCNQYGIKKVR SIPKNITVVSGTDLVLPCHLSSNLAHAHWTFGSQDLPAEQPGSFLYDTGLQALVVMAAQSRHSGPYRCYS EEQGTRLAAESYLVAVVAGSSVTLEARAPLENLGLVWLAVVALGAVCLVLLLLVLSLRRRLREELEKGAK ASERTLVYPLELPKEPASPPFRPGPETDEKLWDPVGYYYSDGSLKIVPGHARCQPGGGPPSPPPGIPGQP LPSPTRLHLGGGRNSNANGYVRLQLGGEDRGGSGHPLPELADELRRKLQQRQPLPDSNPEESSV TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001126047 |
| ORF Size | 2502 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001126047.3, NP_001119519.1 |
| RefSeq Size | 3805 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 20353 |
| UniProt ID | Q64151 |
| Gene Summary | This gene encodes a member of the semaphorin family of proteins that have diverse functions in neuronal development, heart morphogenesis, vascular growth, tumor progression and immune cell regulation. Lack of the encoded protein in some mice causes exencephaly resulting in neonatal lethality. Mice that bypass exencephaly show no obvious behavioral defects but display distinct pigmentation defects. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221937 | Sema4c (untagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C (Sema4c), (10ug) |
CNY 10,640.00 |
|
| MR226572 | Sema4c (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C (Sema4c) |
CNY 9,160.00 |
|
| MR226572L3 | Lenti ORF clone of Sema4c (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C (Sema4c) |
CNY 10,260.00 |
|
| MR226572L4 | Lenti ORF clone of Sema4c (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C (Sema4c) |
CNY 10,260.00 |
