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Pard3 (NM_001013581) Mouse Tagged ORF Clone
CAT＃: MG225840
- TrueORF®
Pard3 (tGFP-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001013581" in other vectors (4)
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CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA960621; AI256638; Asip; D8Ertd580e; Par-3; Par3; Pard-3; Pard3a; Phip |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225840 representing NM_001013581
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTTCATGTCCGCCGGAGCAGCGACCCAGCTTTAACTGGCCTTTCCACTTCTGTCAGTGATAACA ACTTTTCCTCAGAGGAGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCA GAACACCGCTGGAAGTCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTTCCACGGGAT CCCAGTAGCTGGTCCAACCAGTTCCAGCGAGACAACGCCCGCTCCTCCCTGAGCGCCAGCCACCCAATGG TAGACCGGTGGCTGGAGAAGCAAGAACAGGATGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCC GGTTGGACATGCTGATACCGGATTGGAGAACATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTA CAAGTCCCCAACGATGGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACAT TGGGGTTGTTAGTGAAGCGGTTGGAGAAAGGCGGTAAGGCTGAGCAAGAAAACCTTTTCCATGAGAATGA CTGCATTGTGAGGATTAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAACATATGTTCCGC CAAGCTATGCGTGCGCGTGTCATTTGGTTCCATGTGGTCCCTGCAGCAAACAAGGAGCAATATGAACAAC TGTCCCAACGCGAGAAGAACAACTACTCCCCAGGCCGCTTCAGCCCTGACAGCCACTGTGTGGCCAACAG GAGTGTGGCCAACAATGCCCCTCAAGCATTGCCCAGAGCACCCAGACTGAGTCAGCCACCCGAGCAGCTG GATGCTCACCCCCGACTACCTCATAGTGCTCACGCCTCAACCAAACCACCCGCAGCCCCGGCCTTGGCTC CACCCAGTGTGCTTAGTACCAACGTAGGCAGTGTGTACAACACGAAGAAAGTAGGCAAGAGGCTCAACAT CCAGCTTAAGAAAGGTACAGAAGGACTGGGATTCAGCATCACCTCCCGGGACGTCACCATAGGTGGCTCA GCTCCCATTTATGTCAAGAATATCCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGACTCAAGGCAGGAG ACCGGCTAATAGAGGTCAATGGAGTAGATTTAGCAGGCAAATCCCAGGAGGAAGTTGTTTCCCTGTTGAG AAGCACCAAGATGGAGGGGACTGTGAGCCTTCTGGTCTTTCGTCAGGAAGAGGCTTTCCACCCAAGGGAA ATGAATGCTGAACCAAGCCAGATGCAGACTCCAAAAGAAACGAAAGCTGAAGATGAGGACGTTGTTCTCA CACCCGATGGTACCAGGGAGTTTCTGACTTTTGAAGTTCCACTGAATGACTCAGGATCTGCAGGGCTTGG TGTCAGTGTCAAGGGGAACCGTTCCAAAGAGAACCACGCAGATTTGGGGATCTTCGTTAAATCCATTATC AATGGTGGAGCTGCATCTAAAGATGGAAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAATGGAGAAT CTCTACTGGGCAAAGCCAACCAGGAAGCCATGGAGACTCTACGGAGGTCCATGTCCACCGAGGGCAACAA GCGTGGCATGATCCAGCTCATTGTGGCGAGGCGGATCAGCAGATGTAACGAGCTGCGGTCTCCTGGGAGC CCTGCTGCACCTGAGCTGCCCATCGAGACAGAACTGGATGACCGAGAACGCAGGATCTCACACTCCCTCT ACAGTGGGATCGAGGGGCTGGATGAGTCGCCCACCAGGAACGCAGCACTGAGCAGGATAATGGGTAAATG CCAGCTGTCCCCCACGGTGAACATGCCTCACGATGACACTGTCATGATTGAAGATGACAGGCTGCCTGTG CTCCCTCCTCACCTCTCTGACCAGTCCTCCTCCAGCTCCCATGATGACGTTGGATTCATAATGACAGAAG CAGGCACGTGGGCCAAAGCTACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGT TCTCGCTTTTCAACGGGAAGGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAGAT GCCAGTCAATTGGATTTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGGTATAGCTGACGAGACTA AACTCAATACAGTGGATGACCAGAGAGCAGGCTCTCCCAGTAGAGATGTGGGACCCTCCTTAGGTCTGAA GAAATCTAGCTCCTTAGAAAGTCTGCAGACCGCTGTCGCCGAGGTGACGCTGAACGGGAACATTCCTTTC CACCGCCCTCGGCCACGAATCATCCGGGGAAGGGGCTGCAACGAAAGCTTCAGAGCCGCCATTGACAAGT CCTACGACAAGCCCATGGTTGATGATGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAGGAAAG CTCGAGGTCAGGGAGGGAGTCCGTGTCCACGTCCAGCGATCAGCCTTCCTATTCTCTGGAGAGGCAAATG AATGGAGACCCAGAGAAAAGGGACAAGACAGAGAGGAAAAAGGACAAAGCCGGAAAGGATAAGAAGAAAG ACCGAGAGAAGGAGAAGGATAAACTGAAAGCCAAGAAGGGGATGCTGAAAGGCTTGGGGGACATGTTCAG CCTGGCCAAACTGAAGCCGGAGAAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225840 representing NM_001013581
Red=Cloning site Green=Tags(s) MPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRD PSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLV QVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFR QAMRARVIWFHVVPAANKEQYEQLSQREKNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQL DAHPRLPHSAHASTKPPAAPALAPPSVLSTNVGSVYNTKKVGKRLNIQLKKGTEGLGFSITSRDVTIGGS APIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPRE MNAEPSQMQTPKETKAEDEDVVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSII NGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGS PAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGKCQLSPTVNMPHDDTVMIEDDRLPV LPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSD ASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPF HRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQM NGDPEKRDKTERKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFSLAKLKPEKR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013581 |
| ORF Size | 2688 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013581.3 |
| RefSeq Size | 3233 bp |
| RefSeq ORF | 2691 bp |
| Locus ID | 93742 |
| UniProt ID | Q99NH2 |
| Gene Summary | Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222474 | Pard3 (untagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 1, (10ug) |
CNY 11,590.00 |
|
| MR225840 | Pard3 (Myc-DDK-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 1 |
CNY 8,930.00 |
|
| MR225840L3 | Lenti ORF clone of Pard3 (Myc-DDK-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 1 |
CNY 10,830.00 |
|
| MR225840L4 | Lenti ORF clone of Pard3 (mGFP-tagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 1 |
CNY 10,830.00 |
