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Tsc2 (NM_011647) Mouse Tagged ORF Clone
CAT＃: MG225119
- TrueORF®
Tsc2 (tGFP-tagged) - Mouse tuberous sclerosis 2 (Tsc2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_011647" in other vectors (2)
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CNY 15,580.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nafld; Tcs2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225119 representing NM_011647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCAACAAGCAAAGATTCCGGCTTGAAGGAGAAGTTCAAGATACTGTTGGGATTGGGAACAT CGAGGCCAAATCCCAGGTGTGCAGAAGGCAAACAGACTGAGTTTATCATCACATCGGAAATCTTGAGAGA ACTGAGTGGTGAATGCGGCCTCAACAATCGCATCCGAATGATAGGGCAGATCTGTGATGTGGCAAAAACT AAGAAGCTTGAGGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC CACCAGAGGCCCGGCATGCAGTTCTCACCTTATTGAAGGCCATTGTACAGGGACAGGGTGATCGTTTGGG AGTTCTCAGAGCCCTCTTCTTCAAAGTGATCAAGGACTACCCCTCCAATGAAGACCTCCATGAAAGGCTA GAAGTTTTTAAGGCCCTCACAGACAATGGGAGGCACATTACCTATTTGGAAGAAGAACTGGCAGAGTTTG TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTTGTCAACCTGGTCAAGTTCAA CAGCTGTTACCTTGACGAATACATTGCATCAATGGTTCACATGATTTGTCTGCTATGCATCCGGACAGTG TCCTCTGTGGACATTGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCTTACCAGCCG AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCATCAATGTCAAGGAGCTGTGTGAGCCTTGCTG GAAGTTGATGCGTAACCTTCTGGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCGCATCATG GAGGACAGATCCTACATGGAAGATGCCCCACTGCTGAGAGGAGCTGTGTTCTTTGTAGGGATGGCACTCT GGGGAGCTCACCGGCTCTACTCTCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCTAT GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTATAGG AAGGAGCTCCAGGCTGTGACATGGGATATTCTGCTGGACATCATTGAACGACTACTTCAGCAACTCCAGA ACCTGGACAGCCCGGAACTCAAGACCATCGTCCATGACCTGCTGACCACTGTGGAGGAGCTATGTGACCA GAACGAGTTCCATGGCTCGCAGGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGACCTGAA TCCTCTCTCTTAAACTTGATATCCTACAGAGCCCAGTCCATCCACCCTGCCAAGGATGGCTGGATCCAGA ACTTGCAGTTGTTGATGGAGAGGTTCTTCAGGAATGAGTGCCGCAGCGCCGTGCGCATCAAGGTGTTGGA TGTTCTGTCCTTCGTGCTGCTGATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCGGTGGTCATC TCGCAGCTCTCCCACATTCCCGAGGATAAGGACCATCAGGTCCGAAAGCTGGCTACTCAGCTGCTGGTGG ACCTGGCAGAGGGGTGCCACACCCACCACTTCAACAGTCTGCTGGACATCATTGAAAAGGTGATGGCGCG CTCACTCTCTCCACCCCCGGAGCTGGAAGAAAGGGACCTGGCCATGCACTCGGCCTCCCTGGAGGACGTG AAGACCGCGGTCCTGGGGCTCCTGGTCATCCTTCAGACCAAGCTCTACACCTTGCCTGCCAGCCACGCCA CTCGAGTGTATGAGAGCCTCATTAGTCACATCCAGCTCCATTACAAGCACGGCTACTCCCTGCCCATTGC TAGCAGCATCCGACTACAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC CTGCCCAACAAGGATGGGGTCGTGAGATTCAGCCCTTACTGCCTCTGTGACTGCATGGAACTGGATAGAG CCTCAGAGAAGAAAGCCAGTGGGCCCCTTTCACCTCCAACTGGGCCCCCTAGCCCTGTGCCTATGGGCCC TGCTGTGCGGCTCGGCTACCTACCCTACTCCCTGCTCTTCCGTGTCCTGTTGCAGTGTTTGAAGCAGGAG AGCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAGGCTGCCGGAGTCACTGCGCTACAAAGTCCTCATCT TCACCTCCCCCTGCAGTGTCGACCAGCTGTCTTCTGCCCTCTGCTCCATGCTTTCAGCTCCAAAGACCCT TGAGCGGCTCCGAGGTACCCCAGAAGGCTTCTCGAGAACTGACCTGCACTTGGCTGTGGTTCCCGTGCTA ACAGCATTAATATCTTATCACAACTATTTGGACAAGACCAGACAGCGTGAGATGGTGTACTGCCTGGAAC AAGGCCTTATCTACCGCTGCGCCAGCCAGTGCGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA CATCATCATCAAGGCGCTTCCTGTCCTGGTGGTGAAGCTCACACACATTTCTGCCACAGCCAGCATGGCC ATCCCGCTCCTGGAGTTCCTGTCTACTTTGGCCAGGCTGCCCCACCTCTACAGGAACTTTGCTGCAGAGC AGTATGCCAGCGTGTTTGCCATCTCCTTGCCATACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG TCTGGCTCACCACGTCATAGCCATGTGGTTCATCAGGTGCCGACTGCCCTTTCGGAAGGATTTCGTCCCT TATATCACTAAGGGTTTGCGTTCCAATGTCCTCCTGTCTTTTGATGATACCCCTGAGAAGGACAGTTTCA GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGTTTGAGGATAGCCAGAGCCCCCAAACAAGGCCT GAATAACTCTCCACCTGTGAAAGAATTCAAAGAGAGCTGTGCAGCCGAGGCCTTCCGGTGCCGCAGCATC AGTGTATCTGAACATGTGGTCCGCAGCAGAATACAGACATCCCTTACGAGCGCCAGCCTGGGGTCTGCGG ATGAGAACTCTATGGCCCAGGCTGATGACAACTTGAAGAATCTCCACTTGGAGCTCACAGAAACATGTCT GGACATGATGGCCCGATATGTGTTCTCCAATTTCACTGCAGTCCCCAAGAGGTCCCCTGTGGGAGAGTTC CTCCTGGCAGGGGGTAGGACCAAAACCTGGCTGGTTGGAAACAAGCTTGTCACTGTGACAACAAGTGTGG GAACTGGTACACGGTCGCTGCTGGGCCTGGACTCTGGGGACCTGCAGGGTGGCTCGGATTCAAGCTCTGA TCCTAGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTGGGCAGCAGGTG TCCCGTGGGGCCCGGGACCGGGTCCGCTCCATGTCAGGGGGCCATGGCCTTCGAGTTGGTGTCCTGGATA CTTCAGCTCCCTATTCCCCAGGTGGCTCTGCTTCTCTGGGACCACAGACGGCGGTGGCAGCGAAGCCCGA GAAGCCCCCTGCAGGAGCCCAGCTTCCAACAGCAGAGAAGACGAATCTGGCAGCCTATGTGCCTTTGTTA ACCCAGGGCTGGGCAGAAATCTTAGTCCGCAGACCCACAGGAAACACCAGCTGGCTGATGAGCTTGGAGA ACCCGCTCAGCCCCTTCTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCCAATGCCCTTATGGC TGCTGAACGTTTCAAGGAGCACCGGGACACGGCCCTGTACAAGTCATTGTCAGTGCCAGCGGCTGGCACC GCCAAGCCTCCTACTCTCCCACGCTCTAATACAGACTCAGCCATGGTCCTTGAGGAGGGAAGTCCAGGAG AGACTCAGGTGCCAGTGGAGCCCCCCGAATTGGAGGATTTCGAGGCGGCGTTAGGTACAGACAGGCACTG CCAGCGGCCTGACACCTACAGCCGGTCATCCTCAGCATCTAGCCAGGAAGAAAAGTCCCATTTGGAGGAG CTTGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTGCCGTGGACCTCT CCTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCGGAGTTGCAGACCTTACAGGACATCCT TGGAGACCTAGGGGACAAGATTGATATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGGTCCCAGTCAGGG ATCCTGGATGGGGAAGCAGCTACCTGGTCAGCTACGGGTGAAGAGAGCCGTATCACAGTCCCACCTGAAG GTCCTCTGCCTTCCAGTTCTCCCCGCTCCCCCAGTGGCCTCCGGCCCCGAGGCTATACCATCTCTGATTC CGCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGATAACTTCAAGAGCAGAGCTGCGGCCTCCAGTGCT GAGAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCGCCCTTTTTTGGTGATG AGTCCAATAAGCCCATCCTATTGCCCAATGAGTCCTTTGAACGGTCAGTACAGCTGCTTGACCAGATTCC ATCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTGAGCTGGCCATC CTGTCAAATGAGCATGGCTCTTACAGGTACACGGAGTTTCTGACAGGCCTGGGTCGGCTTATTGAGCTCA AGGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTATGTGGCGAGGATGGACAGTTCACCTA CTGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCCACCCTGATGCCCACCAAGGATGTGGAC AAGCACCGCTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAATGATTCTGGTG AGGACTTCAAACTGGGCACCATTAAGGGCCAGTTCAACTTTGTCCATGTGATCATCACACCGCTGGACTA CAAGTGCAACCTATTGACCCTGCAGTGCAGGAAAGACATGGAAGGCCTCGTGGACACCAGTGTGGCCAAG ATTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATATGGCCTCACAGG TACACCACAGCCGATCCAACCCCACTGACATCTATCCCTCCAAGTGGATCGCAAGACTCCGCCACATTAA GCGTCTCCGCCAGAGGATCCGTGAAGAGGTGCACTATTCCAACCCAAGCTTGCCTCTGATGCACCCTCCA GCCCACACCAAAGCCCCAGCTCAAGCCCCTGAGGCTACACCCACCTATGAAACAGGCCAGCGGAAGCGCC TCATTTCCTCCGTGGATGACTTCACAGAGTTTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225119 representing NM_011647
Red=Cloning site Green=Tags(s) MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKT KKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERL EVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTV SSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIM EDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYR KELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPE SSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVI SQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDV KTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLG LPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQE SDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVL TALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMA IPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVP YITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSI SVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEF LLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDSSSDPSTHVRQTKEAPAKLESQAGQQV SRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAAKPEKPPAGAQLPTAEKTNLAAYVPLL TQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAAGT AKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTDRHCQRPDTYSRSSSASSQEEKSHLEE LAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQDILGDLGDKIDIGRLSPEAKVRSQSG ILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDNFKSRAAASSA EKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAI LSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVD KHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAK IVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREEVHYSNPSLPLMHPP AHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011647 |
| ORF Size | 5355 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011647.3, NP_035777.2 |
| RefSeq Size | 6147 bp |
| RefSeq ORF | 5358 bp |
| Locus ID | 22084 |
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