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Dgkh (NM_001081336) Mouse Tagged ORF Clone
CAT＃: MG222176
- TrueORF®
Dgkh (tGFP-tagged) - Mouse diacylglycerol kinase eta (Dgkh), (10ug)
ORF Plasmid: DDK
"NM_001081336" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5930402B05Rik; D130015C16; DGK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222176 representing NM_001081336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGCCGGCAGCCAGCACCACCCTCAGGGCGTCGCGGGAGGAGCGGTCGCTGGGGCCAGCGCGG TTTCCCCCACTGCTGCAGGACCGGGAGAGGATTCGTCCGACAGTGAAGCGGAGCAGGAGGGGCCCCAGAA GCTGATCCGTAAAGTGTCCACCTCTGGACAGATGCGGACCAAGACTAGTATTAAGGAAGGACAACTGCTG AAGCAAACCAGCTCTTTCCAAAGGTGGAAGAAGAGATACTTCAAGCTTCGCGGCCGCACACTTTATTATG CGAAAGACTCCAAGTCCTTGATATTTGATGAAGTTGACCTCTCAGATGCCAGTGTTGCGGAAGCAAGCAC AAAAAATGCCAACAACAGCTTTACGATCATCACTCCGTTCAGAAGACTGATGCTATGTGCTGAGAACAGG AAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAGAACCCTATGAGGTGGCCCAGT TCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGGCCCACCTTCTGTAA CGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGTGAAGTGTGTAAGTTCAAGGCT CATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGCTGGCCTCCATCGGGAAGGACA TCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGGCAACTTGCCTGTGAGTGCCAA GTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGATTGGAAATGCCTTTGGTGTAAG ACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCCTTGGCCAGTGTAAAGTCTCCA TCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCGAGCAACCTTTTCGTTCTGTGT CAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGAGTGAAGTTCCTTCGTCGCTTT AAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGCCTTATTTAGGCTTGAGATTGT TTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAGTGTAGGCTGGGTTTTGTCAGA GATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCCTTGGGTACCGGCAATGACCTT GCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCCCTCAGATACTAGAGAAGCTGG AACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGAGCTCAAATTGCCAGCAAAGTC GTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACAATTTATGAAGACTCCGTTGCA AACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATATCGTCTGCCAAGATACTCTGTG AAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAACACTGGAAAATACAGTAGTAGC CGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAGTTGCTACAAGCCTTGCACGCG GACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCGAAGAGGACACCGTGGAGTCCG CCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACATTGGGAAACCCTCCTCCCAGAA AGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAAGCGGTGAGGCAAGTCATTGAA GAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAGTCAGTCCGTCTTGTGACTATG ATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATCAGCCAAAACCACATCTCAGTC TCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCTGGTCCAGCCATGGCCACCACC AAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGAGAGCAGGACTTGCTGCCTCCA TTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCCTTTCGGGGCAACACCGTTTAT TGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATGAACAACTACTTTGGGATTGGA TTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACCCTGAAAAATGCAGAAGTCGAA CTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACAGAGATCGTACAAGAATCTGGA GCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGTCTACAAGGCATAGCGGTGTTG AACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAGAGGATGATATATTTGCTGCGC CATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGTGCAGATGGCAGTATCAAGGGT CATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATCACGATATTTGGCGACGAGGGA GTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCATCAAGATTGTGCACAAAAACA GAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTCTTGGGAAGACAAGCAGAAGTG TGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCCCCTGACCTGGCAACAGAGGAA GTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTAGGATTTGTGACGCAGCCACGA TTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTCACACGCCCTGAACAAAGCCAA CCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCCATCAACGTCAAGGCGCTCTAT AATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAATCTCCTCACGAAGAGCGGGTGT CCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGATTCCCTGGCTTTATTACATCCT CCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAACAACAAAAGCACAGTCTTTCGC ATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTTCGCAGCCTGGACCTGGGGATT CCGAAAGTGGGTCATATGAAGCGAATTCTCCAGGGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222176 representing NM_001081336
Red=Cloning site Green=Tags(s) MAGAGSQHHPQGVAGGAVAGASAVSPTAAGPGEDSSDSEAEQEGPQKLIRKVSTSGQMRTKTSIKEGQLL KQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENR KEMEVWISSLKSVQSREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKA HKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCK TMVHTACKDVYHPVCPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRF KQLLNPAQVFDLMNGGPYLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLHKQCQLGVLPLGTGNDL ARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPAKSSLLPEPVAATEEFYMTIYEDSVA NHLTKIVNSDEHAVVISSAKILCETVKDFVAKVEKAQDRTLENTVVAEAVASKCSVLNEKLEQLLQALHA DSQASRVPPGIGPAIPEEDTVESASDESLGESKDQLVNDIGKPSSQKAVKPREIMLRANSLKKAVRQVIE EAEKVMDEPAVQPSEPVSPSCDYDTETDEAKEDDAKESLSAKTTSQSPDAQASCGHPQTDSVAGPAMATT KENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSLDGYSEKCVMNNYFGIG LDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVL NIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAVFDSVQMAVSRVIKLQHHRIAQCRTVKITIFGDEG VPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTNLYIHPAPDLATEE VSQMRLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRFPESLTRDTATEIAINVKALY NETEALLVGRVPLHLESPHEERVSSALHSVEMELQKLTEIPWLYYILRPSEDEEPPLDCTKRNNKSTVFR IVPKFKKEKAQKQKTSSQPGPGDSESGSYEANSPGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081336 |
| ORF Size | 3468 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081336.1, NP_001074805.1 |
| RefSeq Size | 3967 bp |
| RefSeq ORF | 3543 bp |
| Locus ID | 380921 |
| UniProt ID | D3YXJ0 |
| Gene Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:27643686). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223782 | Dgkh (untagged) - Mouse diacylglycerol kinase, eta (Dgkh), (10ug) |
CNY 14,920.00 |
|
| MR222176 | Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 8,048.00 |
|
| MR222176L3 | Lenti ORF clone of Dgkh (Myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 11,500.00 |
|
| MR222176L4 | Lenti ORF clone of Dgkh (mGFP-tagged) - Mouse diacylglycerol kinase, eta (Dgkh) |
CNY 11,500.00 |
