Notch3 (NM_008716) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW229011; hpbk; N3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225270 representing NM_008716 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGGGGGCCCGGGGCCGCCGCCGCCGTCGTCGCCTGATGGCCTTGCCACCGCCACCACCGCCCA TGCGGGCGCTGCCCCTGCTGCTGCTGCTAGCGGGGCTGGGGGCTGCAGCACCCCCTTGTCTGGATGGAAG CCCATGTGCAAATGGAGGTCGGTGCACCCACCAGCAGCCCTCCCTGGAGGCTGCTTGCCTGTGCCTGCCA GGCTGGGTGGGTGAGCGGTGCCAGCTGGAAGACCCTTGCCACTCAGGCCCTTGTGCTGGCCGAGGCGTTT GCCAGAGTTCAGTGGTGGCGGGCACCGCCCGATTCTCCTGTCGTTGTCTCCGTGGCTTCCAAGGCCCAGA CTGCTCCCAGCCAGACCCCTGCGTCAGCAGGCCCTGTGTTCATGGTGCCCCCTGCTCAGTGGGGCCGGAT GGCCGATTTGCCTGTGCCTGCCCACCTGGCTACCAGGGTCAAAGCTGCCAAAGTGACATAGATGAGTGCC GATCTGGTACAACTTGCCGTCATGGTGGTACCTGTCTCAATACACCTGGATCCTTCCGCTGCCAGTGTCC TCTTGGTTATACAGGGCTGCTGTGTGAGAACCCCGTAGTGCCCTGTGCCCCTTCCCCGTGTCGTAATGGT GGCACCTGTAGGCAGAGCAGTGATGTCACATATGACTGTGCTTGCCTTCCTGGCTTCGAGGGCCAGAACT GTGAAGTCAACGTGGATGACTGTCCTGGACATCGGTGTCTCAATGGGGGAACGTGTGTAGACGGTGTCAA TACTTACAACTGCCAGTGCCCTCCGGAGTGGACAGGCCAGTTCTGTACAGAAGATGTGGATGAGTGTCAG CTGCAGCCCAATGCCTGCCACAATGGGGGTACCTGCTTCAACCTACTGGGTGGCCACAGCTGTGTATGTG TCAATGGCTGGACGGGTGAGAGCTGCAGTCAGAATATCGATGACTGTGCTACAGCCGTGTGTTTCCATGG GGCCACCTGCCATGACCGTGTGGCCTCTTTCTACTGTGCCTGCCCTATGGGGAAGACAGGCCTCTTGTGT CATCTGGATGATGCATGTGTCAGCAACCCCTGCCATGAGGATGCTATCTGTGACACAAACCCTGTGAGTG GCCGGGCCATCTGCACCTGCCCACCTGGCTTCACTGGAGGGGCATGTGACCAGGATGTGGATGAGTGCTC GATTGGTGCCAACCCCTGTGAACATTTGGGTCGGTGTGTGAATACACAGGGCTCATTCTTGTGCCAATGT GGCCGTGGCTATACTGGACCTCGCTGTGAGACTGATGTCAATGAGTGTCTCTCCGGGCCCTGCCGCAACC AGGCCACGTGTCTTGACCGAATTGGCCAGTTTACTTGCATCTGCATGGCAGGCTTCACAGGGACCTACTG TGAGGTGGACATCGACGAATGTCAGAGCAGCCCATGTGTCAATGGTGGTGTCTGCAAGGACAGAGTCAAT GGCTTCAGCTGCACCTGCCCATCAGGATTCAGTGGGTCCATGTGTCAGCTGGATGTGGATGAGTGTGCAA GCACTCCCTGCCGGAATGGTGCCAAGTGTGTGGACCAGCCTGACGGCTATGAGTGTCGCTGTGCAGAGGG CTTTGAGGGCACTTTGTGTGAGCGAAACGTGGATGACTGCTCTCCGGATCCCTGCCACCACGGGCGCTGT GTCGATGGCATTGCTAGCTTCTCGTGTGCTTGTGCCCCAGGCTATACGGGCATACGCTGTGAGAGCCAGG TGGATGAGTGCCGCAGCCAGCCCTGTCGATATGGGGGCAAATGTCTAGACTTGGTGGACAAGTACCTCTG CCGTTGTCCTCCCGGAACCACAGGTGTGAACTGTGAAGTCAACATTGATGACTGTGCCAGTAACCCCTGT ACCTTTGGAGTTTGCCGTGATGGCATCAACCGTTATGACTGTGTCTGTCAGCCTGGATTCACAGGGCCCC TCTGCAACGTGGAGATCAATGAGTGTGCATCCAGCCCATGTGGAGAGGGTGGCTCCTGTGTGGATGGGGA AAATGGCTTCCACTGCCTCTGTCCACCTGGCTCCCTGCCTCCACTTTGCCTACCTGCGAACCATCCCTGT GCCCACAAGCCCTGTAGTCATGGAGTCTGCCATGATGCACCAGGCGGGTTCCGCTGTGTTTGTGAGCCCG GGTGGAGTGGCCCTCGCTGTAGCCAGAGCCTGGCTCCAGATGCCTGTGAGTCCCAGCCCTGCCAGGCTGG TGGCACCTGCACCAGTGATGGAATAGGCTTTCGCTGCACCTGTGCCCCTGGATTCCAGGGCCATCAGTGT GAGGTGCTGTCCCCCTGTACTCCAAGCCTCTGTGAGCACGGAGGCCACTGTGAGTCTGACCCTGACCGGC TGACTGTCTGTTCCTGTCCCCCAGGCTGGCAAGGCCCACGATGCCAGCAGGATGTGGATGAATGTGCCGG TGCCTCACCCTGCGGCCCCCATGGTACCTGCACCAACCTGCCAGGGAATTTCAGGTGCATCTGCCACAGG GGATACACTGGCCCCTTCTGTGATCAAGACATTGACGACTGTGACCCCAACCCGTGCCTCCATGGTGGCT CCTGCCAGGATGGCGTGGGCTCCTTTTCCTGTTCTTGCCTCGACGGCTTTGCTGGTCCTCGCTGTGCCCG AGATGTGGACGAATGTCTGAGCAGCCCCTGTGGCCCTGGCACCTGTACTGATCACGTGGCCTCCTTCACC TGTGCCTGTCCACCTGGTTATGGAGGCTTCCACTGTGAGATTGACTTGCCGGACTGCAGCCCCAGTTCCT GCTTCAATGGAGGGACCTGTGTGGATGGCGTGAGCTCCTTCAGCTGTCTGTGTCGCCCCGGCTACACAGG CACACACTGCCAATACGAGGCTGACCCCTGCTTTTCCCGGCCCTGTCTGCACGGGGGCATCTGCAACCCC ACCCACCCAGGATTTGAATGCACCTGCCGGGAGGGCTTCACTGGGAGTCAGTGTCAGAACCCAGTGGACT GGTGCAGCCAGGCACCCTGTCAGAATGGGGGTCGCTGTGTCCAGACTGGGGCTTACTGCATTTGTCCACC TGGATGGAGTGGCCGCCTGTGCGACATACAAAGCCTGCCCTGCACGGAGGCCGCAGCCCAGATGGGGGTG AGGTTGGAGCAGCTGTGTCAGGAAGGTGGAAAGTGCATAGACAAGGGCCGCTCCCACTACTGTGTGTGTC CAGAGGGCCGTACGGGTAGTCACTGTGAACACGAGGTGGATCCCTGCACGGCCCAGCCTTGCCAGCACGG GGGCACTTGCCGTGGTTACATGGGGGGCTATGTGTGTGAGTGTCCAGCTGGCTATGCTGGTGACAGTTGT GAGGATAATATAGATGAGTGTGCTTCCCAGCCCTGCCAGAACGGAGGCTCCTGTATCGATCTTGTGGCCC GCTATCTCTGTTCCTGTCCCCCTGGCACACTGGGAGTTCTCTGTGAGATCAATGAGGACGACTGTGACCT AGGCCCATCCTTGGACTCAGGCGTTCAGTGCCTACACAATGGCACCTGTGTGGACCTGGTGGGTGGCTTC CGCTGTAACTGTCCCCCAGGATACACAGGTCTGCACTGTGAGGCAGACATCAATGAGTGTCGCCCGGGTG CCTGCCATGCAGCGCATACTCGGGACTGCCTACAAGATCCAGGTGGGCATTTCCGCTGCGTCTGCCATCC TGGCTTCACAGGGCCTCGCTGTCAGATTGCTCTGTCCCCCTGTGAGTCCCAGCCATGTCAGCATGGAGGC CAGTGCCGTCACAGCCTAGGCCGTGGAGGTGGGCTGACCTTCACCTGTCACTGTGTCCCGCCATTCTGGG GTCTGCGTTGTGAGCGGGTGGCACGCTCTTGCCGAGAGCTGCAGTGCCCAGTGGGTATCCCATGCCAGCA GACAGCCCGTGGACCACGCTGCGCTTGTCCTCCGGGGCTGTCCGGGCCCTCCTGCCGGGTTTCTAGGGCG TCACCCTCAGGAGCTACTAACGCCAGCTGCGCCTCTGCCCCTTGTCTGCATGGGGGCTCATGCCTACCTG TACAGAGTGTCCCTTTCTTCCGCTGTGTGTGCGCTCCGGGCTGGGGCGGCCCGCGTTGTGAGACCCCTTC CGCAGCCCCTGAGGTCCCCGAGGAGCCACGGTGCCCGCGAGCGGCTTGCCAGGCCAAGCGAGGGGACCAG AACTGCGATCGTGAGTGCAACACCCCAGGCTGTGGCTGGGATGGCGGTGACTGCTCACTGAACGTGGACG ACCCCTGGAGGCAGTGTGAGGCACTGCAGTGCTGGCGTCTCTTCAACAACAGCCGGTGTGACCCGGCCTG CAGCTCTCCAGCCTGCCTCTATGACAACTTTGACTGCTACTCTGGTGGCCGCGACCGCACCTGCAACCCT GTTTATGAGAAGTACTGCGCCGACCACTTTGCAGATGGCCGTTGTGACCAGGGCTGCAACACTGAGGAAT GCGGCTGGGATGGGCTGGACTGTGCCAGCGAGGTCCCGGCCCTTTTGGCCCGAGGGGTTCTGGTCCTCAC AGTTCTTCTGCCTCCTGAAGAGTTGCTGCGCTCCAGTGCCGACTTTCTGCAGCGACTCAGCGCTATTCTG CGCACCTCACTGCGCTTCCGCTTGGACGCACGTGGCCAGGCCATGGTCTTCCCCTATCACCGGCCAAGCC CTGGCTCTGAATCCCGGGTCCGTCGTGAGCTGGGTCCTGAGGTGATCGGCTCTGTGGTGATGCTGGAGAT TGACAACCGGCTCTGTCTGCAGTCAGCTGAGAATGACCACTGCTTCCCTGATGCCCAGAGTGCTGCTGAC TACCTGGGAGCCTTGTCAGCAGTGGAGCGACTTGATTTCCCATACCCACTTCGGGATGTGCGAGGAGAGC CGCTGGAGGCCCCAGAGCAGAGCGTGCCACTGCTGCCACTGCTGGTGGCAGGGGCTGTCTTTCTACTCAT CATCTTCATCCTGGGTGTCATGGTTGCCAGGCGAAAGCGAGAACACAGCACCCTCTGGTTCCCTGAGGGT TTTGCATTACACAAGGACATAGCTGCTGGCCACAAGGGCCGGAGGGAGCCTGTGGGACAAGATGCACTGG GAATGAAGAACATGGCCAAGGGTGAGAGTCTGATGGGGGAGGTGGTCACAGACTTGAATGACTCAGAATG TCCAGAGGCCAAGAGACTGAAGGTAGAGGAGCCGGGCATGGGTGCAGAGGAGCCTGAGGACTGTCGCCAG TGGACCCAACACCACCTGGTTGCTGCTGATATCCGTGTGGCACCAGCCACAGCGCTGACTCCTCCTCAGG GAGATGCAGATGCAGACGGAGTGGATGTCAACGTCCGAGGGCCTGATGGCTTCACCCCACTTATGCTGGC CTCCTTCTGTGGGGGAGCCCTGGAGCCGATGCCAGCTGAGGAGGATGAGGCGGATGACACATCAGCCAGC ATTATCTCAGATCTGATCTGTCAAGGGGCCCAGCTCGGGGCACGGACTGACCGCACTGGCGAGACCGCCC TGCATTTGGCTGCCCGCTATGCTAGAGCGGATGCAGCCAAGCGTCTCCTGGATGCTGGGGCGGACACCAA CGCCCAGGATCATTCGGGCCGCACCCCCCTGCACACCGCAGTGACAGCTGATGCCCAGGGTGTCTTCCAG ATTCTCATCAGGAACCGCTCCACTGACCTGGATGCCCGAATGGCAGATGGCTCTACTGCACTGATCCTGG CAGCCCGCCTGGCAGTGGAGGGCATGGTGGAAGAGCTCATCGCCAGCCATGCCGATGTCAATGCAGTGGA TGAGCTTGGGAAATCTGCCTTACACTGGGCTGCAGCTGTGAACAACGTGGAGGCTACCTTGGCTCTGCTG AAAAATGGAGCCAACAAGGACATGCAGGACAGCAAGGAAGAGACGCCGCTGTTCTTGGCCGCTCGGGAGG GCAGCTATGAGGCTGCCAAGCTGCTGCTGGATCATCTCGCCAACCGGGAGATCACAGATCACTTGGACAG GCTGCCCCGGGACGTGGCCCAGGAGCGGCTGCACCAGGACATTGTGCGGTTGCTGGACCAGCCCAGTGGA CCTCGAAGTCCCTCTGGTCCCCATGGCTTAGGGCCATTGCTCTGCCCACCAGGGGCCTTCCTTCCTGGCC TCAAAGCGGTGCAGTCTGGGACCAAGAAGAGCAGGAGGCCACCTGGCAAGACCGGGCTGGGGCCACAGGG AACTCGTGGTCGGGGCAAGAAGCTGACACTAGCCTGTCCAGGACCTCTGGCAGACAGCTCTGTCACACTG TCACCGGTGGACTCTCTGGACTCACCACGGCCTTTCAGTGGGCCCCCTGCTTCCCCTGGAGGCTTCCCCT TGGAGGGCCCCTATGCCACCACGGCCACCGCGGTGTCCTTGGCACAGCTAGGCGCAAGTAGGGCGGGTCC TCTGGGGCGCCAGCCTCCTGGGGGCTGTGTGCTCAGCTTTGGTCTGCTCAATCCTGTAGCTGTTCCCCTC GACTGGGCCAGGCTGCCTCCACCTGCCCCTCCAGGGCCCTCATTCCTGCTGCCCCTGGCTCCGGGACCCC AGTTGCTCAACCCAGGAGCCCCAGTTTCTCCCCAAGAGCGGCCCCCACCCTACCTGGCTGCTCCAGGACA TGGAGAGGAATATCCTGCAGCAGGAACCCGCAGTAGCCCCACCAAGGCCCGCTTCCTGCGGGTTCCCAGC GAGCATCCTTATTTGACCCCGTCTCCTGAGTCCCCAGAGCACTGGGCCAGCCCATCCCCCCCATCCCTCT CAGACTGGTCTGACTCAACACCTAGCCCAGCAACTGCTACCAATGCCACAGCCTCTGGAGCCCTGCCTGC TCAGCCACACCCCATATCTGTTCCCTCCCTCCCTCAGTCCCAGACTCAGCTGGGACCCCAACCAGAAGTT ACCCCCAAGAGGCAGGTGATGGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_008716
Insert Size	6957 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008716.2, NP_032742.1
RefSeq Size	7943 bp
RefSeq ORF	6957 bp
Locus ID	18131
UniProt ID	Q61982
Gene Summary	Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play a role during CNS development.[UniProtKB/Swiss-Prot Function]

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