Wilms Tumor Protein (WT1) (NM_024424) Human 3' UTR Clone

CAT#: SC213657

3' UTR clone of Wilms tumor 1 (WT1) transcript variant B for miRNA target validation



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CNY 4,845.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (2)
Firefly luciferase assay kit, 150 assays
    • 1 kit

CNY 1,520.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Product Name Wilms Tumor Protein (WT1) (NM_024424) Human 3' UTR Clone
Vector pMirTarget
Synonyms AWT1; GUD; NPHS4; WAGR; WIT-2; WT33
ACCN NM_024424
Insert Size 1313 bp
Sequence Data
>SC213657 3’UTR clone of NM_024424
The sequence shown below is from the reference sequence of NM_024424. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
AACATGACCAAACTCCAGCTGGCGCTTTGAGGGGTCTCCCTCGGGGACCGTTCAGTGTCCCAGGCAGCA
CAGTGTGTGAACTGCTTTCAAGTCTGACTCTCCACTCCTCCTCACTAAAAAGGAAACTTCAGTTGATCT
TCTTCATCCAACTTCCAAGACAAGATACCGGTGCTTCTGGAAACTACCAGGTGTGCCTGGAAGAGTTGG
TCTCTGCCCTGCCTACTTTTAGTTGACTCACAGGCCCTGGAGAAGCAGCTAACAATGTCTGGTTAGTTA
AAAGCCCATTGCCATTTGGTGTGGATTTTCTACTGTAAGAAGAGCCATAGCTGATCATGTCCCCCTGAC
CCTTCCCTTCTTTTTTTATGCTCGTTTTCGCTGGGGATGGAATTATTGTACCATTTTCTATCATGGAAT
ATTTATAGGCCAGGGCATGTGTATGTGTCTGCTAATGTAAACTTTGTCATGGTTTCCATTTACTAACAG
CAACAGCAAGAAATAAATCAGAGAGCAAGGCATCGGGGGTGAATCTTGTCTAACATTCCCGAGGTCAGC
CAGGCTGCTAACCTGGAAAGCAGGATGTAGTTCTGCCAGGCAACTTTTAAAGCTCATGCATTTCAAGCA
GCTGAAGAAAAAATCAGAACTAACCAGTACCTCTGTATAGAAATCTAAAAGAATTTTACCATTCAGTTA
ATTCAATGTGAACACTGGCACACTGCTCTTAAGAAACTATGAAGATCTGAGATTTTTTTGTGTATGTTT
TTGACTCTTTTGAGTGGTAATCATATGTGTCTTTATAGATGTACATACCTCCTTGCACAAATGGAGGGG
AATTCATTTTCATCACTGGGAGTGTCCTTAGTGTATAAAAACCATGCTGGTATATGGCTTCAAGTTGTA
AAAATGAAAGTGACTTTAAAAGAAAATAGGGGATGGTCCAGGATCTCCACTGATAAGACTGTTTTTAAG
TAACTTAAGGACCTTTGGGTCTACAAGTATATGTGAAAAAAATGAGACTTACTGGGTGAGGAAATCCAT
TGTTTAAAGATGGTCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGTGTTGTGTTTTGTTTTTT
AAGGGAGGGAATTTATTATTTACCGTTGCTTGAAATTACTGTGTAAATATATGTCTGATAATGATTTGC
TCTTTGACAACTAAAATTAGGACTGTATAAGTACTAGATGCATCACTGGGTGTTGATCTTACAAGATAT
TGATGATAACACTTAAAATTGTAACCTGCATTTTTCACTTTGCTCTCAATTAAAGTCTATTCAAAAGGA
AA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq NM_024424.5
Synonyms AWT1; GUD; NPHS4; WAGR; WIT-2; WT33
Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Locus ID 7490
MW 49.8
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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