Swine IgG (H&L) Antibody Peroxidase Conjugated

Specifications

Product Data
Product Name	Swine IgG (H&L) Antibody Peroxidase Conjugated
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:100,000 - 1:200,000
Reactivity	Porcine
Host	Goat
Immunogen	Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	2.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Note	Anti-Swine IgG Peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 µg of Swine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

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