Bovine IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunoblotting (1/2,000-1/10,000). ELISA (1/20,000-1/100,000). Immunohistochemistry (1/1,000-1/5,000). Immunomicroscopy. as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.  This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Immunogen	Bovine IgG whole molecule
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.