CD11b (ITGAM) Rabbit Polyclonal Antibody

CAT#: TA336565

Rabbit Polyclonal Integrin alpha M/CD11b Antibody



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CNY 6,056.00


货期*
5周

规格
    • 100 ul

经常一起买 (4)
Transient overexpression lysate of integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2
    • 100 ug

CNY 4,840.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
CNY 1,430.00


Recombinant protein of human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, 20 µg
    • 20 ug

CNY 2,900.00


Recombinant protein of human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, 100 µg
    • 100 ug

CNY 9,998.00

Specifications

Product Data
Applications FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution Dual RNAscope ISH-IHC, Immunohistochemistry: 1:400, Simple Western: 1:50, Western Blot: 2 ug/mL, Single Cell Western: 1:10, Flow (Cell Surface): 1:10 - 1:1000, Immunohistochemistry-Paraffin: 1:400, Immunocytochemistry/ Immunofluorescence: 1:200, Immunohistochemistry-Frozen, In-situ Hybridization, Flow Cytometry
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Formulation PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 160 kDa
Gene Name integrin subunit alpha M
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6
Note This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916)
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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