Vinculin (VCL) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
Reactivity	Human, Mouse, Rat
Modifications	Phospho-specific
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyrosine 821
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	lot specific
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	123 kDa
Gene Name	vinculin
Database Link	NP_003364 Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human UniProt ID P18206
Background	Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and a-actinin. In addition, vinculin, Talin and a-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression.
Synonyms	CMD1W; CMH15; HEL114; MV; MVCL
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

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