GOT1 Sheep Polyclonal Antibody

CAT#: R1087

GOT1 sheep polyclonal antibody, Serum



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CNY 20,681.00


货期*
4周

规格
    • 2 ml

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
Recommended Dilutions:
ELISA: 1/5,000-1/25,000.
Western Blot: 1/500-1/3,000.
This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Goat anti-Sheep IgG [H&L] (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
Reactivity Porcine
Host Sheep
Clonality Polyclonal
Immunogen Aspartate Aminotransferase (AST) from Porcine Heart.
Specificity Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
Cross reactivity against Aspartate Aminotransferase (AST) from other tissues and species may occur but have not been specifically determined.
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative.
State: Serum
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification Prepared from monospecific antiserum by a Delipidation and Defibrination.
Conjugation Unconjugated
Storage Condition Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Background Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Synonyms Aspartate aminotransferase, Transaminase A
Note Aspartate Aminotransferase (AST) is also referred to as Glutamic Oxaloacetic Transaminase (GOT).
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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