Rad51L1 (RAD51B) Mouse Monoclonal Antibody [Clone ID: OTI7H4]

Specifications

Product Data
Clone Name	OTI7H4
Applications	FC, IF, WB
Recommend Dilution	WB 1:500, IF 1:100, FLOW 1:100
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1(NP_598193) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	42 kDa
Gene Name	RAD51 paralog B
Database Link	NP_598193 Entrez Gene 19363 MouseEntrez Gene 5890 Human UniProt ID O15315
Background	The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
Synonyms	R51H2; RAD51L1; REC2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination

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