basic Cytokeratin Mouse Monoclonal Antibody [Clone ID: Ks pan 1-8]

Specifications

Product Data
Clone Name	Ks pan 1-8
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen and cytological material. Working Dilution: 1/10 with PBS, pH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT.
Reactivity	Amphibian, Bovine, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal proteins from cultured Human MCF-7 cells
Specificity	This antibody represents an excellent marker for distinguishing carcinomas from non-epithelial tumors. Polypeptides Reacting: Mr 52 500-Mr 68 000 keratins (type II keratins K1-K8; formerly also designated Cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: All epithelium-derived neoplasms. Tested Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, Bovine BMGE+H, BMGE-H, MDBK.
Formulation	Final solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Reference Data

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