APAF1 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1 μg/ml. Immunohistochemistry on paraffin sections 2 μg/ml.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1
Specificity	This antibody detects APAF1 (N-term). No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	apoptotic peptidase activating factor 1
Database Link	Entrez Gene 11783 MouseEntrez Gene 78963 RatEntrez Gene 317 Human UniProt ID O14727
Background	Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23.1 It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.2 This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9.3 Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death.4 Additionally, APAF1 has been shown to interact with NLRP1,5 Caspase-9,5, 6, 7, 8, 9, 10 APIP,6 BCL2-like 18, 9 and HSPA4.10
Synonyms	Apaf-1, KIAA0413
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Huntington's disease, p53 signaling pathway, Parkinson's disease, Small cell lung cancer

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